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Citations for New England Biolabs :
2851 - 2900 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The region harboring the randomized hexamer and flanking constant tags was amplified from 1 µg ssDNA for 5 cycles using the Q5 Hot Start High-Fidelity PCR Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs M0531) and PCR cycling at 98°C for 30s ...
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bioRxiv - Genetics 2021Quote: ... 10 ug of gel-purified mononucleosome DNA was prepared for high-throughput sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB). Sequencing was performed by the University of Oregon Genomics and Cell Characterization Core Facility.
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bioRxiv - Genomics 2021Quote: ... Second PCR amplification with 8 cycles was used for generating sequencing-ready libraries using NEBNext High Fidelity master mix and Illumina Universal and Index primers (E7335S, New England Biolabs) in four and twelve parallel reactions on M-280 beads for day 2 and day 8 samples ...
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bioRxiv - Genomics 2021Quote: ... 10ng of cDNA is circularized using a DNA splint compatible with 10X cDNA and the NEBuilder HIFI DNA Assembly Master Mix (NEB). The DNA splint was generated by primer extension of the following oligos:
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bioRxiv - Genomics 2021Quote: ... Adapters with barcode provided by Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd, Oxford, UK) were then ligated using the NEB Blunt/TA Ligase Master Mix (NEB). After purification with AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
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bioRxiv - Synthetic Biology 2021Quote: ... were amplified from pSerOTSλ and placed under the control of the glnS* promoter and assembled with the kanamycin selection marker and origin of replication from pSerOTSλ by gibson assembly using NEBuilder HiFi DNA Assembly master mix (NEB). tRNA expression cassettes (1x- ...
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bioRxiv - Biochemistry 2020Quote: DNA fragments containing sgRNA sequences were amplified from isolated genomic DNA by two rounds of PCR using NEBNext Ultra II Q5 master mix (NEB). For the first round of PCR ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA and incorporated into a NotI-digested linear pDSP11 vector backbone using the NEBuilder HiFi DNA Assembly master mix (New England Biolabs) to yield the vectors pSC1 and pSC2 ...
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bioRxiv - Bioengineering 2020Quote: ... A HiFi assembly reaction was then performed to join the PCR product with the linear pEERM1 to form the assembled circular plasmid by incubating at 50 °C for one hour using NEBuilder DNA HiFi Assembly Master Mix (New England Biolabs). The HiFi reaction solution (5 μL ...
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bioRxiv - Biochemistry 2020Quote: ... The insert and vector DNA were combined in a 3:1 insert:vector molar ratio in a volume of 5 μL and added 15 μL of Gibson Assembly Master Mix (New England BioLabs), consisting of T5 Exonuclease ...
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bioRxiv - Neuroscience 2021Quote: ... The 3’UTRs were then cloned into the cut plasmid via Gibson Assembly using the Gibson Assembly Master Mix (New England Biolabs). The 3’UTRs of ppk and chic were amplified by RT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction was performed using a 50 µL master mix consisting of 1x standard Taq buffer (New England Biolabs), LCO1490 primer (0.2 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 20 uL 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and using touchdown PCR cycling at 98°C for 30s ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP and input DNA libraries were prepared for amplification by using the NEBNext Ultra II ChIP-seq Library Prep Master Mix Set for Illumina (NEB) following manufacturer’s protocols with no modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... WGS libraries were amplified using NEBNext® Ultra™ II Q5® Master Mix and index primers (New England Biolabs). Library purification was performed with TIANgel Midi Purification Kits (TIANGEN Biotech) ...
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bioRxiv - Cell Biology 2021Quote: ... CK1γ3 kinase dead mutants were generated using PCR mutagenesis and cloned into pDONR223 digested with BsrGI using Gibson Assembly Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... Homology arms were then cloned into an SEC backbone plasmid (pJF250, pDD282, or pLC019) with Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB). A mixture of the modified SEC repair plasmid (25 ng/μl ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into SR54 proGL2:EYFP cleaved with SalI and KpnI using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) with gene-specific primers (Supplemental Table 5) ...
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bioRxiv - Plant Biology 2021Quote: ... fragment of pAPL (fig. S6C) were generated according to the NEBuilder HiFi DNA Assembly Master Mix protocol (New England Biolabs). Modified DOF(II ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were gel-purified and used for a second round of PCR amplification (Q5 NEB master mix, 7 cycles) using custom primers to attach Illumina read sequences ...
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bioRxiv - Neuroscience 2021Quote: RT-PCR was conducted with 15ng cDNA input in a 100ul reaction using NEBNext Ultra II Q5 Master Mix (New England Biolabs), with resulting products visualized on a 1.5% TAE gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... The double-stranded template was produced by annealing the strands and filling in the single-stranded regions with Phusion HF PCR Master Mix (NEB) by cycling between the melting temperature of the strands and 72°C twenty times ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using oAC158/oAC159 and inserted into BamHI digested miniCTX1-optRBS-lacZ (14) using isothermal assembly (Gibson Assembly Master Mix, New England BioLabs). The reactions were transformed into chemically competent DH5α and selected for on tetracycline ...
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bioRxiv - Microbiology 2021Quote: ... Polymerase chain reactions were performed with 15 μL Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, USA), 0.2 μM of forward primer ...
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bioRxiv - Microbiology 2021Quote: ... The inducible pSCrhaB2-BCAL2345-his6 and pSCrhaB2-BCAL2466-his6 constructs were generated using Gibson assembly 39 by inserting PCR amplified fragments into NdeI and XbaI linearized pSCrhaB2 using NEBuilder® HiFi DNA master mix according to the manufacturer’s instructions (New England Biolabs). pKM4 Site directed mutagenesis was undertaken using PCR-based site replacement and DpnI digestion 40 ...
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bioRxiv - Neuroscience 2020Quote: ... Engineered SiR vectors carrying d.C1349G or d.G1357T PEST-targeting mutations were produced by PCR amplification of the Rabies genome in 2 fragments starting from the end of N assembled using Gibson master mix (NEB).
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bioRxiv - Microbiology 2021Quote: ... Restriction enzyme-cut pTOX3 was incubated with purified AB and CD PCR products along with a half-reaction of HiFi DNA Assembly Master Mix (NEB) according to manufacturer’s directions ...
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bioRxiv - Genomics 2021Quote: ... and used to prepare libraries using the Nextera XT Dual-Indexed primer system (Nextera) and NEBNext High-Fidelity PCR Master Mix (New England Biolabs). Libraries were sequenced by the UCSD Institute for Genomic Medicine on an Illumina HiSeq 4000 using paired end reads of 100bp.
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bioRxiv - Microbiology 2021Quote: ... 2000 fmoles of the gRNA library oligonucleotide pool were mixed with 50 fmoles of linearized CROP-seq-opti in 10μl of NEB-Builder master mix (New England Biolabs # E2621). After 1 hour incubation at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using an Native Barcoding Expansion 96 kit (Oxford Nanopore Technologies) and a Blunt/TA Ligation Master Mix (New England Biolabs). Barcoded samples (96 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the templates were prepared by polymerase chain reaction (PCR) amplification from corresponding plasmid constructs using Q5 DNA Polymerase Master Mix (NEB). The PCR reaction was worked up using a Qiagen PCR purification kit ...
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bioRxiv - Microbiology 2019Quote: ... and the ptrA cassette were assembled with the pYES2 backbone using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB) and incubated at 50°C for 1 hour to form the new pCDH-TagBFP-T2A-myc-BirA*-Tensin3 construct ...
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bioRxiv - Bioengineering 2020Quote: ... Respective peptide DNA coding sequences (CDS) were amplified from hACE2 via PCR and inserted using HiFi DNA Assembly Master Mix (NEB) for Gibson Assembly into the pcDNA3-SARS-CoV-2-S-RBD-Fc backbone linearized by digestion with NheI and BamHI ...
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bioRxiv - Genomics 2021Quote: Both pegRNA and TAPE constructs were cloned either using Gibson assembly (Gibson Assembly Master Mix, New England Biolab) or ligation after restriction (T4 DNA Ligase, New England Biolabs). For the Gibson assembly protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Ends were subsequently ligated by adding a 900 μL master mix containing 120 μL 10X T4 DNA ligase buffer (NEB), 100 μL 10% TritionX-100 ...
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bioRxiv - Biochemistry 2022Quote: TrcP protein expressing vector pET28-TrcP was generated by integrating trcPORF and a linearized pET28 using the NEBuilder HiFi DNA Assembly Master Mix (NEB). The mutant derivatives of this vector were generated with the PrimeSTAR Mutagenesis Basal Kit (TAKARA-bio).
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bioRxiv - Cell Biology 2022Quote: ... Plin5 truncations and Chimeric Plin5 or Fatp4 constructs were generated using HiFi DNA Assembly Master mix (New England Biolabs, E2621). Plin5 mutant constructs were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: 1.0 pmol of oligo pool and 0.005 pmol linearized plasmid were mixed with NEBuilder® HiFi DNA Assembly Master Mix (E2621, New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The mutated genomic sequences were subcloned back into pGWB553 vectors using Gibson assembly with NEBuilder Hifi DNA Assembly Master Mix (NEB). The WT constructs included a C-terminal mRFP tag (Ishikawa et al. ...
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bioRxiv - Biophysics 2022Quote: ... using 10 ng of purified plasmid as the template for each 25 μl reaction and 25 PCR cycles with the Q5 Master Mix (New England Biolabs). Amplicons from the clarified culture supernatant (“SUP” ...
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bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 2.5 ng of the input RNA was subjected to 35 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... and a volume of cDNA corresponding to 5 ng of the input RNA was subjected to 30 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Neuroscience 2022Quote: ... DNA templates were generated for each sgRNA by annealing oligonucleotides using the NEBNext Ultra II Q5 master mix (NEB, M0544L). The HiScribe Quick T7 kit (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 fmol of input cDNA were incubated for 20 minutes at room temperature (15–25°C) with the native barcodes and Blunt/TA Ligase Master Mix (New England Biolabs), followed by 10 minutes at 65°C.
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bioRxiv - Genetics 2022Quote: ... [10] using AUB516 + AUB517 and cloned into the HindIII site of pYES2 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) following the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... DNA library was prepared by PCR amplification using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs). The resulting PCR products were purified by QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... with 0.1 pmol of ~60bp ssDNA oligo (either oBK9102 or oBK9104) with 10 μL NEBuilder HiFi DNA Assembly Master Mix (NEB) in a final volume of 20 μL ...