Labshake search
Citations for New England Biolabs :
2451 - 2500 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... Fragments were assembled using the NEBuilder HiFi DNA Assembly Master Mix (Cat. Nr. E2621, New England Biolabs).
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bioRxiv - Genetics 2020Quote: ... genomic DNAs from AAV9-sgRNA infected SCs were amplified using Q5 High-Fidelity 2× Master Mix (NEB). PCR products were purified through QIAQuick PCR purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Fragments were then assembled into lentiGuide Neo using Gibson Assembly Master Mix (New England BioLabs Inc., MA) according to manufacturer instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were built by Gibson Assembly using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs, # E2621). After assembling ...
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bioRxiv - Molecular Biology 2020Quote: ... The UMI adapters ligation reaction contained 50 µL Blunt/TA ligation Master mix (New England Biolabs, USA), 20 µL adaptor mix prepared above and 1 µg end-repaired DNA in 80 µL nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1×loading buffer (contained SYBR green ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion®High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1X loading buffer (contained SYB green ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the libraries for Illumina whole-genome sequencing were prepared with NEBNext master mix kits (New England Biolabs), and the sequencing was done with Illumina HiSeq2000 (Mattila et al ...
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bioRxiv - Molecular Biology 2019Quote: ... and ligated into linearized pENTR-OsU3-sgRNA-NGG using Sticky-End Ligation Master Mix (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: The TIR1-hygro vector was assembled by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB, E2621L) replacing puromycin gene by hygromycin gene in the pEN396-pCAGGS-Tir1-V5-2A-PuroR (gift of Elphege Nora ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs), and the mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... the fragments were assembled into the a linearized pMA backbone using HiFi DNA Assembly Master Mix (NEB) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB). Cloning was considered successful when sequence identify >99.5% was given ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid DE was assembled via Gibson assembly (Gibson Assembly Master Mix of New England BioLabs, Inc.). The assembly was performed at equimolar concentrations of linearized plasmid MinDpUC57 and MinE DNA fragments for 1 hour at 50 °C ...
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bioRxiv - Genetics 2021Quote: ... Plasmid and genomic DNA sequences were amplified using Q5 Hotstart Master Mix (New England Biolabs, Cat. #M0494S) and Gibson assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly was conducted per the manufacturer’s instructions (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs), followed by transformation of the product into E.coli DH5α ...
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bioRxiv - Microbiology 2020Quote: ... Selected barcodes for each sample were ligated using the Blunt/TA Ligase Master Mix (New England Biolabs) and the resulting fragments were purified using AMPure XP beads ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Add 948 μl of ligation master mix: 150 μl 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μl 10% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... Gibson assembly for each scFv was performed using the NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) according to manufacturer’s instructions with BamHI linearized pYD1 vector and scFv fragment in 1:4 ratio (in molarity) ...
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bioRxiv - Bioengineering 2022Quote: ... and fragments assembled by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB; catalog # E2621L). Plasmids pIS002 and pIS003 were also constructed using Gibson Assembly similarly to pIS001 ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2022Quote: ... 30μl of master mix composed by 5μl of 5U/μl Klenow polymerase (New England Biolabs cat. #M0210L), 0.75μl of each 10mM dCTP ...
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bioRxiv - Plant Biology 2022Quote: ... Gibson Assembly was performed according to the Gibson Assembly® Master Mix Kit catalogue (New England Biolabs). priDZ123 and priDZ124 ...
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bioRxiv - Synthetic Biology 2022Quote: ... colony PCRs were performed using the commercial OneTaq™ master mix (New England BioLabs; Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosisbioRxiv - Molecular Biology 2022Quote: Four hundred nanograms of the amplicon was incubated with NEBuilder HiFi DNA Assembly Master Mix (NEB, USA) in a 20ul reaction at 50°C for 60 minutes.
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bioRxiv - Genomics 2022Quote: ... 25 μL of 2xKAPA NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Cat. M0543L), and 0.5μL of 100x SYBR Green I dye (FMC BioProducts ...
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bioRxiv - Plant Biology 2024Quote: ... after the CaMV35S promoter (p35S) by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB). The resulting plasmids containing p35S::CYP73A412-TurboID-EGFP ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Microbiology 2024Quote: ... All LAMP reactions were conducted in the WarmStart® Colorimetric LAMP Master Mix (NEB Canada, Whitby, ON). Each 20-µ L reaction contained 2 µ L of template ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation of the Native barcode was then performed by adding Blunt/TA Ligase Master Mix (NEB M0367) and a unique native barcode (Oxford Nanopore Technologies EXP-NBD196 ...
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bioRxiv - Neuroscience 2024Quote: ... Inserts were cloned into KpnI– and AgeI-digested AAV backbones using HiFi DNA Assembly Master Mix (NEB). All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... whose cDNA was assembled from three fragments using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S). A TAF4 G/C- rich fragment was synthesized as gene block with reduced G/C content (IDT).
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bioRxiv - Genomics 2024Quote: ... Genomic DNA libraries were amplified using 1X NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544), 1X EvaGreen dye (Biotium 31000) ...
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bioRxiv - Genomics 2024Quote: ... along with a 4.8uL 3:2 master mix of T4 ligase buffer:T4 ligase (New England Biosciences, NEB) and 9.4uL of nuclei buffer with BSA (NBB ...
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bioRxiv - Cell Biology 2023Quote: ... All sequences were inserted into L4440 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into DH5a bacteria ...
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bioRxiv - Systems Biology 2023Quote: ... for the screens with EpiC and Tiling libraries and NEBNext Ultra II Q5 Master Mix (NEB, #M0544) for the screens with T1 library ...
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bioRxiv - Microbiology 2023Quote: ... PCR product was assembled with EcoRI-linearized pBAD L24 using NEBuilder HiFi DNA Assemly Master Mix (NEB). Strep-Tag II and His-tag C-terminally modified SAMase coding plasmids were constructed in a similar manner ...
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bioRxiv - Microbiology 2022Quote: ... and then the adapters were ligated using the NEB Blunt/TA Ligase Master Mix (New England Biolabs). The Native Barcoding (12 ...
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bioRxiv - Microbiology 2023Quote: ... and AgeI- and PmeI-digested TOP-CAR were assembled using HiFi DNA Assembly Master Mix (NEB #E2621L) to generate the TOP-SLC35A2 expression plasmid.
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bioRxiv - Synthetic Biology 2023Quote: ... constructs were created using the Gibson assembly-based NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs) and transformed into E ...
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bioRxiv - Biochemistry 2023Quote: ... as well as the housekeeping gene (GAPDH) using quick-load Taq 2× master mix (New England Biolabs), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and PCRs were performed via Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB #M0531S). After PCR clean-up (Promega #A9281 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR reactions were performed using Q5 High-Fidelity master mix (New England Biolabs, Ipswich, Massachusetts, USA). For the first stage PCR ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were digested with DpnI and assembled with Gibson assembly master mix (New England Biolabs) according to the manufacturer instructions ...