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Citations for New England Biolabs :
2251 - 2300 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: All plasmids were constructed using either NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) for Gibson assembly28 ...
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bioRxiv - Microbiology 2024Quote: ... Clean deletions were verified via PCR using Quick-Load Taq Master Mix (New England Biolabs) or Q5 High Fidelity Master Mix ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB) with NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB). Barcoded fragments were purified with AMPure XP beads (Beckmann Coulter) ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were generated by PCR with NEBNext Ultra II Q5 Master Mix (NEB M0544) in two consecutive reactions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix and T4 DNA ligase from New England Biolabs (NEB). We obtained oligonucleotides from Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 7.5 ng of plasmid backbone and 2.5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB). We incubated the reaction mix for 16 h at 50 °C ...
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bioRxiv - Genomics 2024Quote: ... All plasmid constructs were made using Gibson assembly master mix (New England Biolabs, Ipswich, MA). Assembled constructs were electroporated into electrocompetent E ...
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bioRxiv - Cell Biology 2024Quote: ... via ligation-independent cloning with the NEBuilder® HiFi DNA Assembly Master Mix (NEB E2621S). Correct plasmid insert sequences were confirmed by Sanger sequencing (University of Utah DNA Sequencing Core ...
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bioRxiv - Neuroscience 2024Quote: ... we introduced mutations by primers and aligned by HiFi DNA Assembly Master Mix (NEB, E2621L). Ligated PCR products inserted into the pNL1.1 vector (Promega ...
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bioRxiv - Immunology 2024Quote: ... a DNA library was generated using NEB Next Ultra II Q5 Master Mix (NEB# M0544L) and amplified by PCR as previously described (21) ...
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bioRxiv - Genomics 2021Quote: ... and libraries were amplified by adding 10 μL DNA to 25 μL of NEBNext HiFi 2x PCR mix (New England Biolabs) and 2.5 μL of 25 μM each of Ad1 and Ad2 primers using 11 PCR cycles ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Purified DNA was used as an input to generate a library by amplifying with 2x Q5 DNA polymerase mix (NEB) and indexing primers ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments were inserted into BsaI-digested GreenGate empty entry vectors [45] via Gibson assembly (2x NEBuilder Hifi DNA Assembly Mix, NEB) or restriction ligation with T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2023Quote: ... All primers contained 30bp homologous regions to allow assembly of PCR fragments into the pUC57 backbone using 2X HiFi assembly mix (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs) for 1h at 50°C ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic DNA inserts were cloned into our plasmid pZE21-ME using 2x NEBuilder HiFi assembly mix (New England Biolabs, E2621L) according to manufacturer instructions (assuming an insert DNA mass of 15 ng and an average fragment size of 2 kb) ...
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bioRxiv - Genomics 2020Quote: ... The L2:DDRGK1 sequence was PCR amplified with Phusion High Fidelity DNA polymerase master mix (NEB), using cDNA generated in the L2:DDRGK1 CRISPRa experiment with gRNA 1 ...
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bioRxiv - Microbiology 2021Quote: ... Assembly of DNA fragments was performed with Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA). E ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat.# E2621S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 μL of NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts, USA). Finally ...
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bioRxiv - Genomics 2020Quote: Libraries were either indexed with NEBNext DNA Library Prep Master Mix for Illumina (New England Biolabs) using 6 µg input 3C DNA as previously described8 or using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... Native barcodes were added by ligation using the Blunt/TA ligase master mix (New England Biolabs) and the ligation reaction was purified using 1 volume ratio of AMPure beads ...
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bioRxiv - Genomics 2020Quote: ... and consecutive sequencing adapter ligation employing the NEB Blunt /TA Ligase Master Mix (New England Biolabs). The enzymatic steps (RT ...
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bioRxiv - Microbiology 2019Quote: ... Amplified backbone and ROP17 were then assembled using the Gibson assembly master mix (New England Biolabs). ElectroMAX DH10B E ...
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bioRxiv - Immunology 2019Quote: ... HDR donor constructs were cloned by Gibson assembly using the Gibson Assembly Master Mix (NEB, E2611S) into the pUC57(Kan ...
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bioRxiv - Synthetic Biology 2019Quote: ... Both fragments were assembled into pCRBlunt-glpK using the NEBuilder HiFi DNA Assembly Master Mix (NEB), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB) or in vivo assembly (31) ...
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bioRxiv - Genomics 2019Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1_noMX universal primer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The sequencing library for DY15505 was constructed using the NEBNext DNA Library Prep Master Mix (NEB). For the other 37 strains ...
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bioRxiv - Microbiology 2021Quote: ... and pEGPN-TMPRSS2 were constructed by Gibson cloning using NEBuilder master mix (New England Biolabs, E2621) with overlapping PCR generated inserts for promoter EF1α ...
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bioRxiv - Biochemistry 2020Quote: ... All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB, M0493S). Reagents for the KLD reactions came from NEB ...
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bioRxiv - Plant Biology 2021Quote: ... using BamHI and XhoI restriction sites and 2× Gibson Assembly master mix (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Using the NEBNext DNA Library Prep Master Mix Set for 454 (New England Biolabs ref: E6070) 21.25μl of DNA extract was subjected to three consecutive steps ...
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer were purchased through New England Biolabs (NEB). ssDNA substrates used for extension reactions were ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs, MA, USA), RNA PCR Primer1 (RP1) ...
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bioRxiv - Microbiology 2020Quote: ... and performed 6–9 cycles of PCR with the NEBNext Ultra II Q5 Master Mix (NEB) using Illumina P7 and the Seq-Well P5-TSO hybrid primer (Gierahn et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Digested and purified insert and vector were ligated using Instant Sticky- end Ligase Master Mix (NEB) following instructions by the manufacturer ...
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bioRxiv - Genetics 2021Quote: All plasmids were constructed using Gibson-cloning with the NEBuilder HiFi DNA Assembly master mix (NEB). For the construction of the N-terminal part of the split NG-ABEmax-encoding vector (AAV-NT-ABEmax) ...
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bioRxiv - Molecular Biology 2021Quote: ... The petSUMO_Nsp1_WT construct was then obtained using PCR-products with Gibson Assembly® Master Mix (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Site directed mutagenesis was performed using the Phusion High-Fidelity PCR master Mix (New England Biolabs). All mutations were confirmed by DNA sequencing.
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bioRxiv - Genomics 2021Quote: ... UK) using Blunt/TA ligase master mix (Cat. No. M0367, New England Biolabs, Ipswich, Massachusetts, USA). The ligation mix was incubated at room temperature for 15 mins followed by AMPure bead purification ...
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bioRxiv - Microbiology 2021Quote: ... Cloning was performed using isothermal DNA assembly with the Gibson Assembly Master Mix (New England Biolabs) [68] ...
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bioRxiv - Microbiology 2021Quote: ... All LAMP reactions were conducted in the WarmStart colorimetric LAMP master mix (NEB Canada, Whitby, ON). Each reaction was 25 µL in total and contained 2 µL template ...
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bioRxiv - Microbiology 2020Quote: All LAMP reactions were conducted in the WarmStart colorimetric LAMP master mix (NEB Canada, Whitby, ON). Each reaction was 25 μL in total and contained 1 μL template ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA, USA) at a 1:2 (vector:insert ...
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bioRxiv - Microbiology 2020Quote: ... and then the two fragments were joined using the NEBuilder HiFi DNA Assembly Master Mix (NEB) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 6.25μl forward and barcoded reverse primer (10μM, IDT) and 25μl Phusion High-Fidelity master mix (NEB). The PCR program was ...
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bioRxiv - Biochemistry 2022Quote: ... and Gibson assembly was accomplished using the NEBuilder HiFi DNA Assembly master mix (New England Biolabs). New England Biolabs restriction enzymes were used to digest DNA ...