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Citations for New England Biolabs :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... The Monarch® RNA purification kit (New England Biolabs, Whitby, ON, Canada) was used to purify synthesized dsRNA following the manufacturer’s recommendation ...
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bioRxiv - Zoology 2024Quote: The complete open reading frames of AedaeCCHa1R and AedaeCCHa2R were amplified by PCR using Q5 high-fidelity DNA polymerase (New England Biolabs, Whitby, ON, Canada) with primers located upstream of the start codon and downstream of the stop codon (Supplement Table S1 ...
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bioRxiv - Zoology 2024Quote: ... genomic DNA was restriction digested with HindIII (New England Biolabs) and circularized by self-ligation ...
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bioRxiv - Zoology 2024Quote: ... Total RNA was then extracted using a Monarch® Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: ... and pMi{Hau-cif7-ngfp}) were produced using NEBuilder HiFi DNA Assembly kit (NEB). The pMi base plasmid was linearized by double digestion with SpeI and SacII ...
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bioRxiv - Biophysics 2024Quote: ... cDNA (2 μl) was used as template in 50 μl PCR reaction with Phusion Hot start flex (New England Biolabs). Reaction conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μL i7 unique index primer (10 μM) and 25 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB) were added to 21 μl of purified CUT&TAG DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was quality checked using Bioanalyser (RIN>9) and libraries generated using NEBNext Ultra II Library Prep Kit (NEB, E7770) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: EM-seq libraries were prepared from genomic DNA as previously (78) using the NEB Enzymatic Methyl-seq kit (New England BioLabs; P7120L). In brief ...
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bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat. R3575, EcoRI-HF NEB cat. R3101, HindIII-HF NEB cat. R31045, SspI-HF Neb cat. R3132 and XbaI NEB cat. R01455). Immunoprecipitation was performed using 8 µg of fragmented DNA incubated for 14h at 16°C with 20 µg of S9.6 antibody (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat. R3575, EcoRI-HF NEB cat ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Biophysics 2024Quote: ... and NotI (NEB, #R3189) in CutSmart buffer (NEB ...
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bioRxiv - Biophysics 2024Quote: M13mp18-derived p7249 scaffold was obtained from NEB (catalog no. N4040S). Unmodified and biotinylated oligonucleotides for the rectangle origamis were obtained from MWG Eurofins ...
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bioRxiv - Biophysics 2024Quote: ... Annealed inserts were ligated into the vector with T4 DNA ligase (NEB, #0202) in T4 DNA ligase buffer for 20 minutes at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... in T4 DNA ligase buffer (#, NEB) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... utilising the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490L) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genomics 2024Quote: ... The directional RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library prep for Illumina kit (NEB, E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Immunology 2024Quote: ... Amplification reactions consisted of 50 μL LongAmp Taq 2x Master Mix (New England Biolabs), 1 μL 10 μM forward primer ...
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bioRxiv - Immunology 2024Quote: ... and 1,000ng of total RNA was treated with the NEBNext poly (A) mRNA Magnetic Isolation Module (NEB, #E7490L). RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L) ...
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bioRxiv - Genetics 2024Quote: ... 40 μl of MNAse digestion buffer (3.33 gelUnits/μl Micrococcal Nuclease (NEB M0247S), 1.25X Micrococcal Nuclease buffer ...
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bioRxiv - Genetics 2024Quote: ... 50 μl of MNAse digestion buffer (5 gelUnits/μl Micrococcal Nuclease (NEB M0247S), 2X Micrococcal Nuclease buffer ...
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bioRxiv - Biochemistry 2024Quote: ... E.coli ECOR47 or E.coli dh5a F’ (NEB, C2992). For protein expression and purification ...
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bioRxiv - Genetics 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... and indexed primers (NEB) and purified with 1.8X Ampure XP beads ...
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bioRxiv - Genetics 2024Quote: ... The DNA (500 ng) was incubated with DNase I (1U, NEB, M0303L), Fast AP (1 U ...
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bioRxiv - Genetics 2024Quote: ... The sheared DNA was used as input for the NEBNext Enzymatic Methyl-seq (NEB E7120) following the manufacturer’s instructions except for doubling the reaction incubation times ...
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bioRxiv - Genetics 2024Quote: ... The looped adaptor ligated DNA fragments were subjected to USER (NEB M5508) digestion at 37 ᵒC for 20 minutes and used directly for bisulfite treatment using the Imprint DNA Modification Kit (Sigma MOD50 ...
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bioRxiv - Genetics 2024Quote: For library construction the ChIP purified DNA was used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645L) with modifications of the manufacturer instruction as we assembled half volume reactions ...
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bioRxiv - Immunology 2024Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Genetics 2024Quote: ... 10 µL 10 mg/mL BSA (NEB cat no. B9000s), 840 μL DNA grade H2O (Invitrogen Waltham ...
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bioRxiv - Genetics 2024Quote: ... then digested with a final concentration of 1 U/μL of DpnII (NEB cat no. R0543S) in 450 μL of 1X digestion reaction buffer ...
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bioRxiv - Biochemistry 2024Quote: RNAs were dephosphorylated using Quick CIP (NEB) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and T4 DNA ligase were purchased from New England Biolabs (NEB). PCR was performed with Taq (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... the PCR cocktail mix (25 µl) consisted of 12.5 µl OneTaq® Quick-Load® (2x Master mix; New England Biolabs), 1.5 µl each of forward (10 µM) ...
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bioRxiv - Biophysics 2024Quote: ... with calf intestinal phosphatase (NEB, #M0290). Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... Inserts were phosphorylated by incubation with T4 polynucleotide kinase (NEB, #M0201) in T4 DNA ligase buffer (# ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was cleaved by XhoI (NEB, #R0146) and NotI (NEB ...
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bioRxiv - Biophysics 2024Quote: ... SNAP-Surface Alexa Fluor 647 (catalog no. S9136S) was obtained from NEB. 40 nm and 90 nm diameter gold nanoparticles (catalog no ...
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bioRxiv - Microbiology 2024Quote: Polymerase chain reactions (PCRs) for cloning purposes were performed using the high fidelity Phusion DNA Polymerase (NEB, France). PCR products were purified with the NucleoSpin PCR Clean Up kit (Macherey Nagel ...
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bioRxiv - Microbiology 2024Quote: ... The resulting DNA sample was then digested by MmeI (NEB) and re-purified on magnetic beads ...
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bioRxiv - Microbiology 2024Quote: ... and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760S/L), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... High-quality RNA samples were used to construct sequencing libraries with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the libraries were sequenced using Illumina HiSeq X ten/NovaSeq 6000 system (2 × 150 bp read length ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) and purified using the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA template was amplified using Q5 polymerase (New England Biolabs, NEB) and purified using the Monarch PCR & DNA Cleanup Kit (NEB) ...