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Citations for New England Biolabs :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... On-bead PCR indexing-amplification was performed using custom-ordered indexing primers (IDT) matching the Illumina Nextera Index Kit sequences and 2x Phusion Master Mix (NEB). PCR reactions were amplified for 9-11 cycles ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Microbiology 2024Quote: ... Inserts were amplified by PCR from diluted ΦKZ lysates and joined into the linearized vector by Hi-Fi DNA Assembly (NEB). The resulting vectors were used to transform E ...
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bioRxiv - Microbiology 2024Quote: ... Genes of interest were PCR amplified to include either their native or heterologous promoter and cloned using Gibson Assembly kit (NEB) into integration vectors pDG1662 (for insertion into the amyE locus) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR fragments were joined together using Gibson assembly kit (NEB Cat. No. E2611L) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... with PvuI-HF (NEB Cat. No. R3150S) and NotI-HF (NEB Cat ...
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bioRxiv - Microbiology 2024Quote: ... of the digested backbone with phosphorylated (NEB Cat. No. M0201L) annealed oligos oAA027-028 ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic libraries were prepared using the NEB Ultra II kit (NEB; Cat. No. E7645L) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol oligonucleotide was incubated with 10 µCi [γ-32P]-ATP in the presence of 10 U T4 polynucleotide kinase (NEB) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Microbiology 2024Quote: ... the protein eluted from MonoQ 5/50 GL was incubated with 10 μM SNAP-Surface Alexa Fluor 647 (NEB) for 1 hour at 4°C prior to gel filtration.
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bioRxiv - Microbiology 2024Quote: ... Recorded data were further processed using CGQquant (Aquila Biolabs) for merging the replicates ...
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bioRxiv - Microbiology 2024Quote: ... the purified PCR products were ligated with the empty vectors in a molar ratio of 1:4 using T4 DNA ligase (NEB, USA) at 16 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were then assembled in an ApaI and SacII digested pSVA407 vector via Gibson Assembly following the manufacturer’s instructions (New England Biolabs). The respective deletion mutants were then transformed with the resulting knock-in plasmids pSVA407-ΔupsE:P1117 saci_1117 CtSS and pSVA407-ΔupsE:P2033 saci_2033-2034 ...
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bioRxiv - Microbiology 2024Quote: ... 7.5µl 2x NEBNext High Fidelity Master Mix (NEB), and 1.25µl of each barcoded Nextera N5x and N7x index primers per reaction ...
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bioRxiv - Microbiology 2024Quote: ... primers oAA033-034 were used to remove mprF using the Q5 site-directed mutagenesis kit (NEB Cat. No. E0554S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 1µl 100mM dNTPs (NEB), plus 100ng of the pDNA and UltraPure water ...
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bioRxiv - Microbiology 2024Quote: ... 50 μg RNA was treated with T4 polynucleotide kinase (New England Biolabs, MA, USA) according to the manufacturer’s instructions at 37°C ...
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bioRxiv - Microbiology 2024Quote: SV40 enhancer was amplified with PCR using Q5 High-Fidelity DNA Polymerase (NEB) from plasmid template ...
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bioRxiv - Microbiology 2024Quote: ... Cells were treated with 200 U/mL of α2,3,6,8,9 neuraminidase A (NEB Bioke, P0722L) and 0.6 U of α1,2,3,4,6-L-fucosidase (Megazyme ...
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bioRxiv - Microbiology 2024Quote: SV40 LT area was amplified with PCR using Q5 High-Fidelity DNA Polymerase (NEB). Template was SV40 genome WT4 plasmid which was received as a gift from James DeCaprio ...
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bioRxiv - Microbiology 2024Quote: ... gel purified with Monarch DNA Gel Extraction Kit (NEB), and ligated with T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... PCR product and pSIP409 plasmid were digested with NcoI and XhoI enzymes (NEB), gel purified with Monarch DNA Gel Extraction Kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Taq 2X Master Mix (New England Biolabs) was used for the reaction and PCR primers are listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplification of the gRNA insert was done on 2000 genomes per sample using Q5® Hot Start High-Fidelity DNA Polymerase (NEB # M0493) with P5 and P7 primers that included the Genewiz partial adapter sequence (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM DTT) and a wash with 10 μL streptavidin (NEB). The chamber was further washed with 10 μL TIRF buffer followed by a 1-minute incubation with 2 μL of microtubules diluted in 8 μL TIRF buffer supplemented with 50 mM KCl and 1.25 mg/mL casein (TIRF-Casein) ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Microbiology 2024Quote: Test PCRs on cDNA were performed in a 50 µL scale using 1.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode ...
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bioRxiv - Microbiology 2024Quote: ... followed by treatment with polynucleotide kinase (NEB). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then purified again and the eluate was subjected to circularization with T4 RNA Ligase 1 (NEB). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using dual-end indexing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645L) using the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, #E7530L). All libraries were prepared using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Q5 Reaction Buffer Pack (NEB, B9027S). For PCR #1 ...
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bioRxiv - Cell Biology 2024Quote: ... one of the immunoprecipitated samples was treated with 400 units of Lambda protein phosphatase (New England Biolabs, P0753S) at 30 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... All PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493L) and the Q5 Reaction Buffer Pack (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... 15µl to 25µl of extracted proteins in NuPAGE buffer and 0.5µl protein ladder (BioLabs) were loaded into a Bio-Rad 4-15% Mini-PROTEAN precast polyacrylamide gel ...
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bioRxiv - Cancer Biology 2024Quote: ... and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253, New England Biolabs) was added and the reaction was incubated at 42 °C for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted and cloned into BsmBI-digested (NEB)/ CIP-treated (NEB) plentiCRISPR v2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted and cloned into BsmBI-digested (NEB)/ CIP-treated (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and underwent paired end sequencing in a NextSeq500 or HiSeq PE150 (Novogene).
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bioRxiv - Cancer Biology 2024Quote: ... and library quantification using NEBNext Library Quant kit (NEB), the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... PolyA RNA was extracted using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used as input for RNA-seq ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5,000 U/mL PNGase F (P0704S, New England BioLabs) in growth media for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB) as described above for StcE and sialidase ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB). After library QC analysis using Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA sequencing library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.