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Citations for New England Biolabs :
2201 - 2250 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The assembly reaction was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... both fragments being then assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Plasmid pMMB-control was built by removing the msrDL-msrD(1-3):yfp cassette via quickchange mutagenesis using primer pair 32/33 ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... and homology based assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) following manufacturer guidelines and transformed into E ...
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bioRxiv - Genomics 2022Quote: ... sgRNA-cloning was performed with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621S) according to manufacturer’s instructions using BbsI-linearization of P2X458 for the first sgRNA and SapI linearization of P2X458 for the second sgRNA as backbone ...
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bioRxiv - Synthetic Biology 2022Quote: ... Assembly of amplicons was performed using the NEBuilder HiFi DNA Assembly Master Mix (NEB, #E2621). Assembled products were transformed into NEB® 10-beta Competent E ...
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bioRxiv - Microbiology 2022Quote: ... Insert and vector fragments were then added to Gibson assembly master mix (New England BioLabs) and incubated at 50°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... using NEBuilder® HiFi DNA Assembly Master Mix per manufacturer’s instructions (New England BioLabs, E2621S). To generate the lentiviruses ...
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bioRxiv - Plant Biology 2022Quote: ... All primers were designed with appropriate overlapping overhangs and HiFi DNA assembly master mix (NEB) was used for cloning ...
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bioRxiv - Physiology 2022Quote: ... Luna Taq Premade Master Mix was used (M3003E, New England Biolabs Canada, Whitby, ON, Canada). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Q5 DNA polymerase and NEBuilder HiFi DNA Assembly Master Mix from New England Biolabs (NEB). Plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Physiology 2022Quote: ... were assembled into FCIV.FM1 vector using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs), creating a plasmid expressing C-terminal FLAG-tagged Prdm13-202 driven by a ubiquitin promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing library was prepared using NEBnext® Ultra II Q5 Master Mix (NEB #M0544) and custom primers (forward ...
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bioRxiv - Plant Biology 2024Quote: ... The NEB Next® DNA Library Prep Master Mix set for Illumina kit (NEB #E6040S) was implemented to prepare the DAP-Seq gDNA library ...
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bioRxiv - Molecular Biology 2023Quote: All plasmids were constructed with NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... C-sbGluc and p2a dTomato by Gibson cloning (New England Biolabs HiFi DNA Master Mix). Coding sequences from plasmids for GCaMP6f ...
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bioRxiv - Cell Biology 2024Quote: ... G or C) and assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The randomized N12-oligonucleotides were commercially synthesized and purified on a reversed-phase column (FASMAC ...
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bioRxiv - Neuroscience 2024Quote: ... whereas Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). All sequences were verified using Sanger sequencing (Microsynth).
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bioRxiv - Molecular Biology 2024Quote: ... at the BsmBI site with Instant Sticky-end Ligase Master Mix (New England Biolabs®), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... to replace the GFP sequence using NEBuilder HiFi DNA assembly master mix (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... RNF43 mutants were generated by PCR-subcloning using Q5 High-Fidelity 2× Master Mix (NEB). Domain swapped constructs were generated by in-fusion cloning (Takara Bio) ...
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bioRxiv - Genomics 2024Quote: ... Endogenous loci were amplified with 1X Q5 High-Fidelity Master Mix (New England Biolabs M0492), 1 M Betaine (MilliporeSigma B0300) ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Then, 2.5 μL of MNase master mix (MNase buffer, 0.125 U/μL MNase (NEB, M0247S), 2 mM DTT ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). pXT7 and pDEST vectors were a kind gift from the group of Laure Bally-Cuif ...
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bioRxiv - Cell Biology 2023Quote: ... The gfp sequence was cloned and incorporated using NEBuilder HiFi DNA Assembly Master Mix (NEB). The col1a2-gfp sequence from the final pGEMT-col1a2-gfp construct was excised using restriction enzymes and inserted into the pTol2 vector ...
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bioRxiv - Synthetic Biology 2022Quote: All cloning was performed by isothermal assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB #E2621), using the plasmid ΔVP1 + VP2C-GFP14 as the backbone vector ...
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bioRxiv - Microbiology 2023Quote: ... Gibson assembly was performed with the NEB Gibson Assembly® Master Mix (New England Biolabs) with a vector-to-insert ratio of 1:5 ...
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bioRxiv - Microbiology 2023Quote: ... Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass pool ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μl of a master mix that contained 1X DNA Adenylation Buffer (New England Biolabs), 100 μM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... the NEBuilder HiFi Master Mix was used according to kit instructions (E5520S, New England Biolabs). All single integration plasmids were digested with PmeI before transformations and integrations were verified by PCR ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... and mutant Pacer RH domain PCR product were mixed with Gibson Assembly master mix (NEB) in a 1:3:3 molar ratio prior to incubation at 50 °C for 15 minutes and transformation into XL10 E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification of the libraries was performed using NEBnext Q5 Ultra II master mix (NEB) and the last sample cleanup was performed by double-sided PEG-magnetic bead purification to eliminate PCR primers and fragments above 1 kb in size ...
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bioRxiv - Molecular Biology 2023Quote: ... and assembled into the pDEST17 backbone with NEBuilder HiFi Assembly Master Mix (NEB, Cat #E2621). Correct assembly of each plasmid was verified by sequencing (Genewiz/Azenta).
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bioRxiv - Microbiology 2023Quote: ... pRYI039 was assembled by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB). The assembly reaction was transformed into chemically competent NEB5α cells and candidate transformants were selected using kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Systems Biology 2023Quote: ... The “splint” ligation reaction was performed with 1X Instant Sticky End Master Mix (NEB #M0370) at 24°C for 1 hour with shaking at 1400 rpm on a ThermoMixer ...
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bioRxiv - Biochemistry 2023Quote: ... This was done by Gibson reaction using the Gibson assembly master mix (New England Biolabs) and mixing the oligonucleotide sequences with the vector in a 4:1 molar ratio ...
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bioRxiv - Biochemistry 2023Quote: ... The-gel purified DNA fragments were assembled according to Gibson Assembly® Master Mix (NEB). The assembled products were transformed using Gibson Assembly Cloning Kit (NEB) ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... PCR amplification was performed with the specific primers and OneTaq master mix (New England Biolabs) under the following condition ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed by combining 12.5 µL Q5 high-fidelity 2× master mix (NEB), 2.5 µL each of 10 µM forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: ... the digested vector and the oligo were assembled using Gibson Assembly Master Mix (NEB #E2611S). After transformation into Stbl3 chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... Library amplification PCRs were performed using NEBNext Ultra II Q5 Master Mix (New England Biolabs) on all the extracted genomic DNA to attach Illumina sequencing adaptors and barcodes ...
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bioRxiv - Plant Biology 2023Quote: ... These two fragments were assembled by NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) to construct pSD8.
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bioRxiv - Neuroscience 2023Quote: ... gel extracted and assembled utilizing a Gibson master mix (New England BioLabs Inc.; Cat#E2611L) to yield f(U6 ...
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bioRxiv - Plant Biology 2023Quote: ... and Gateway-compatible pENTR-MRK1 clones were generated the using Gibson Assembly Master Mix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... occurred alongside library amplification with NEBNext High-Fidelity PCR Master Mix (New England Biolabs, M0541S). The PCR reaction was carried out with the following conditions ...
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bioRxiv - Biochemistry 2023Quote: ... Gibson Assembly Master Mix and Q5 High-Fidelity DNA Polymerase were purchased from NEB (USA). Amicon Ultra-0.5 mL centrifugal units ...
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bioRxiv - Cell Biology 2023Quote: ... APOBEC converted DNA was PCR amplified using 22x cycles with Q5U master mix (NEB M0597S) with primer pairs cTF378+cTF381 for the promoter and oSA021+oSA023 for the gene body ...
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bioRxiv - Neuroscience 2023Quote: ... and plasmid fusion/reassembly were performed with the NEBuilder HiFi DNA Assembly Master Mix (NEB). To create the empty shRNA plasmid (shE) ...