Labshake search
Citations for New England Biolabs :
1201 - 1250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... 125V for 50 min) with SYBR Safe DNA stain (Thermo) using the 1kb Plus DNA ladder (NEB). Gel imaging was performed on a FluorChem M System (Cell Biosciences) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... ssRNA ladder (NEB), RiboRuler Low Range RNA Ladder (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... dsRNA ladder (NEB), ssRNA ladder (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Caspase-1 (Provided by Peter Broz) 39 were generated via PCR and inserted into the pHR backbone via HiFi cloning mix (New England Biolabs). All Melt37/40-Effector fusions were generated by amplifying Melt37/40 with primers that amplified the region downstream of a.a.96 such that the final Melt variants contained a a.a.1-96 deletion ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were circularized via ligation (New England Biolabs). For Melt-effector fusions ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inserts were generated using PCR or gBlock synthesis (IDT) and were ligated either by T4 ligase (NEB #M0202M) or In-Fusion (Takara #102518 ...
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bioRxiv - Synthetic Biology 2024Quote: ... DH10B (NEB) were transformed with the purified plasmid library according to the Library Creation protocol.
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were prepared using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S) for total RNA isolation ...
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bioRxiv - Cancer Biology 2024Quote: ... and mutant histone H2A-K119C was generated using the Q5 mutagenesis kit (NEB). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... The 601 DNA fragment was excised using EcoRV (NEB) and fragments further separated from the vector backbone using repeated steps of PEG precipitation.
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bioRxiv - Neuroscience 2024Quote: ... and 100 ng of RNA was reverse-transcribed into cDNA using the LunaScript RT Master Mix Kit (New England Biolabs #E3025L). qPCR reactions utilized specific primers and were performed in triplicate with Applied Biosystems TaqMan Gene Expression Master Mix (ThermoFisher #4369016) ...
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bioRxiv - Neuroscience 2024Quote: Adult worms from INF418 (nonEx106[myo-2p::GCaMP8f::unc-54 3’UTR]) and INF96 (syIs391[myo-2p::NLS::GAL4SK::VP64::unc-54 3’UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] ...
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bioRxiv - Synthetic Biology 2024Quote: ... pSpy0K6 and p7INT.1 was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs®) according to the manufacturers protocol for Gram-positive bacteria (low input ...
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bioRxiv - Synthetic Biology 2024Quote: pSpy0C2 was cloned using Golden Gate Assembly using the type II restriction enzyme Esp3I (NEB). The MCS-cat fragment and the pUC origin were amplified using OLEC10311/10312 from pSpy1C and OLEC10315/10316 from pUC19-mKate2∼ssrA ...
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bioRxiv - Synthetic Biology 2024Quote: ... Antarctic Phosphatase (NEB #M0289L) and T4 Polynucleotide Kinase (NEB M0201L) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and T4 Polynucleotide Kinase (NEB M0201L). Golden gate assembly and Gibson assembly were also utilized ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes (NEB), T4 DNA Ligase (NEB #M0202L) ...
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bioRxiv - Synthetic Biology 2024Quote: ... See Table S1 for a full list of derived yeast strains. NEB Turbo competent Escherichia coli (E. coli) from New England Biolabs (NEB was used for all DNA cloning and plasmid propagation work.
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bioRxiv - Synthetic Biology 2024Quote: The plasmid DNA was subjected to double digestion using KpnI and XbaI enzymes (New England Biolabs, Ipswich, MA, USA), followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were diluted with Gel Loading Dye (New England BioLabs), and 5 μL was loaded into a 2% agarose gel (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... TurboID was fused at the C-terminus of PSD95 and inserted into a cre-dependent AAV expression vector under the synapsin promoter by Gibson assembly (NEB). Plasmids will be deposited at plasmids.eu.
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Immunology 2024Quote: ... AscI (for U6 fragment) (NEB, #R0558), or SbfI-HF (for crRNA fragment ...
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bioRxiv - Immunology 2024Quote: ... BsaI-HF (NEB, #R3535/R3733), AscI (for U6 fragment ...
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bioRxiv - Immunology 2024Quote: ... and digested using DpnI (NEB, #R0176S), BsaI-HF (NEB ...
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bioRxiv - Immunology 2024Quote: ... Coli (NEB, #C3040) at 42°C and the ligation products were spread onto ampicillin selection plates ...
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bioRxiv - Immunology 2024Quote: ... or SbfI-HF (for crRNA fragment) (NEB, #R3642). pLenti backbone was cut with AscI/SbfI-HF and purified using the QIAquick PCR purification kit ...
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bioRxiv - Molecular Biology 2024Quote: The flow cell chamber was passivated with 30 µL PBS-BSA buffer (1.0 mg/mL BSA (NEB) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Nicks were sealed by adding 10 μL 10x T4 DNA ligase buffer and 2 μL T4 DNA ligase (New England Biolabs) and incubating overnight at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: The flow cell chamber was passivated with PBS-BSA buffer (1.0 mg/mL BSA; NEB) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pAM089 was linearized by cleavage with EcoRI-HF (New England Biolabs), separated on a 1x TBE agarose gel and extracted by electroelution ...
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bioRxiv - Immunology 2024Quote: ... using Q5 polymerase (NEB): 98°C for 30s ...
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bioRxiv - Immunology 2024Quote: ... The remainder of the adapter sequences were added to the heavy and light chains separately by a two-step PCR reaction with Q5 using the NEBNext index primers (NEB) 98°C for 30s ...
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bioRxiv - Immunology 2024Quote: ... Exonuclease I (M0293L, NEB) was used to digest remaining primers following cleanup at 37 °C for 20 minutes followed by 80 °C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... cDNA was used to make libraries with the NEBNext Ultra II FS Library Preparation Kit (E6177S, NEB), using a five-fold lower reaction volume than instructed by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... sample amplification was performed using a library PCR using Q5 Master Mix (M0544L, NEB), 1 µL i7 Index primer (Sigma-Aldrich®) ...
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bioRxiv - Molecular Biology 2024Quote: ... performed with NotI-HF® (NEB, cat. R3189) and AscI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... We then added T4 DNA ligase (New England Biolabs, M0202L) in the presence of 1 mM ATP overnight at 16 °C and subsequently heat-inactivated the next morning for 10 min at 65 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... truncated KQ (NEB, M0373L). The 5’ ends of RNA fragments were phosphorylated by T4 PNK and ligated to 5’ adaptor (CGATCTCCAATTCCCACTCCTTTCAAGACCTrC ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions followed the manufacturer recommended recipe for Phusion High Fidelity Polymerase (New England BioLabs, Inc.) with these PCR conditions ...
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bioRxiv - Microbiology 2024Quote: ... and the Taq DNA Ligase (New England Biolabs) according to a protocol adapted from (Shenoy & Visweswariah ...
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bioRxiv - Microbiology 2024Quote: ... and 3 µl Ultra II end prep enzyme mix (NEB), and incubated at 20 °C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... whereas Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). All sequences were verified using Sanger sequencing (Microsynth).
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bioRxiv - Neuroscience 2024Quote: ... for Illumina (New England Biolabs). For size selection (25-150bp ...
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bioRxiv - Neuroscience 2024Quote: ... the sequencing libraries were generated using a NEBnext® Ultra™ II DNA library prep kit and NEBNext Multiplex Oligos (NEB #E7335) for Illumina (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... the T2A-mCherry sequence was amplified by PCR and added after the second NLS sequence using the NEBuilder HiFi Assembly Master Mix (New England Biolabs). A previously described sgRNA sequence targeting Shank3 (Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... were then ligated using T4 DNA ligase (NEB) according to the standard protocol ...