Labshake search
Citations for New England Biolabs :
1401 - 1450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
-
bioRxiv - Molecular Biology 2024Quote: ... HR template was divided into 3 parts and each part was amplified by NEB Q5 polymerase using AJ51 & AJ52 oligonucleotides for part 1 ...
-
bioRxiv - Neuroscience 2024Quote: ... DNA lysate was used as genotyping PCR template with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and enhancer specific genotyping primer pair spanning the upstream and downstream Cas9-guide RNA cleavage sites (Table S19) ...
-
bioRxiv - Neuroscience 2024Quote: We constructed the input library for MutSTARR-seq by cloning these eBlock fragments into the linearized hSTARR-seq ORI vector using Gibson Assembly Master Mix (NEB). We then introduced the transformed plasmids into MegaX DH10 electrocompetent cells (Invitrogen) ...
-
bioRxiv - Neuroscience 2024Quote: ... The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB). After amplifications ...
-
bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...
-
Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and total RNA was isolated and purified using the Monarch Total RNA Miniprep Kit (New England BioLabs). This purified RNA was then used to prepare sequencing libraries with the Tru-Seq Stranded with RiboZero Gold (Human/Mouse/Rat ...
-
bioRxiv - Molecular Biology 2024Quote: ... gRNA-Cas9 plasmids were generated upon cleavage with BbsI restriction enzyme (NEB, R3539M) of the px330 plasmid following NEB instruction ...
-
bioRxiv - Molecular Biology 2024Quote: ... and specific buffer (NEB, B0202S) following manufacturer instructions ...
-
Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were incubated with 1:50 Endonuclease V (New England BioLabs) in a calcium containing blocking buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5 µL of Rapid PNGase F (New England Biolabs, Fig. 2d and Extended Data Fig. 2a) were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... Patient mutations were introduced using Q5 site-directed mutagenesis Kit (NEB). Transduction was performed as described previously (50).
-
bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by ligation (T4 DNA ligase, NEB). The resulting saci_1116 expression plasmid ...
-
bioRxiv - Molecular Biology 2024Quote: ... The fragment was cloned into pSVAaraFX-saci_1116-CtSS by restriction with SacII and NcoI (NEB) followed by ligation (T4 DNA ligase ...
-
bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
-
bioRxiv - Molecular Biology 2024Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
-
bioRxiv - Molecular Biology 2024Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Half of the fragmented nucleic acids were digested with RNase H (New England Biolabs) overnight at 37°C to serve as a negative control ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Xba I (NEB, R0145L), and EcoRI (NEB ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and EcoRI (NEB, R3101L) overnight at 37°C ...
-
bioRxiv - Genomics 2024Quote: ... The CRISPR-targeted sequences were subsequently amplified using Phusion® High-Fidelity DNA Polymerase (NEB). The primers used for the PCR were as follows ...
-
bioRxiv - Genomics 2024Quote: ... was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
-
bioRxiv - Molecular Biology 2024Quote: ... a master mix containing hydrogel beads carrying DNA stub and ligation mixture was distributed in the same 4 x 96-well plates such that each well would contain 12.5 μl of close-packed hydrogel beads,1.25 µl of 10× ligation buffer (NEB, B0202S), 0.3 μl of T4 DNA ligase I (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... the UGI in pYPQ265E2 was replaced by 2xUGI using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs®) to generate A3A-Y130F-nzCas9-2xUGI ...
-
bioRxiv - Molecular Biology 2024Quote: ... digested by NheI and BamHI were jointed together with 15-20 bp overlapping sequences using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... coli (New England Biolabs), and midi-prepped using a Plasmid Plus Midi kit (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... at the BsmBI site with Instant Sticky-end Ligase Master Mix (New England Biolabs®), respectively ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1 unit of Q5 polymerase (New England Biolabs) were mixed in 1x Q5 buffer to a final volume of 100 µl ...
-
bioRxiv - Immunology 2024Quote: ... we electroporated 100 µl of NEB 10-beta (NEB, #C3020K) in a BioRad electroporator using the preset E ...
-
bioRxiv - Immunology 2024Quote: ... we found cloning was most efficient when we digested the library and plasmid with BsmBI-v2 (NEB, #R0739L) (but not the barcode fragment due to the quantity ...
-
bioRxiv - Immunology 2024Quote: ... and the pHW2000 plasmid backbone such that the whole plasmid could be assembled in a one-pot golden gate reaction with PaqCI (NEB, #R0745S). Due to repeated regions ...
-
bioRxiv - Immunology 2024Quote: ... coli (NEB, #C3040H) at 30 °C in LB medium ...
-
bioRxiv - Immunology 2024Quote: ... dNTPs (NEB, #N0447L, 1 µl), water (1 µl) ...
-
bioRxiv - Immunology 2024Quote: The second PCR step added NEBNext unique dual index primers onto the ends of the amplicon (NEB, #E6440S). This was achieved by a two-step PCR reaction containing the same reagents as in the first PCR step except the primers were 5 µl of the supplied unique dual index primer mix ...
-
bioRxiv - Molecular Biology 2024Quote: ... the reaction was started in the absence of GTP and in the presence of 0.5 mM of anti-reverse m7G-cap analog (NEB #S1411) for 10 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (NEB Next UltraII, New Endland Biolabs M5044S). i5 and i7 multiplexing barcodes were introduced in a second round of PCR and final gel purified products were sequenced on Illumina NextSeq500 systems to determine sgRNA representation in T0 and T18 time points of each sample ...
-
bioRxiv - Molecular Biology 2024Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (NEB Next UltraII ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA was recovered by melting the agarose plugs and then digesting the agarose with β-agarase enzyme (NEB, M0392L), using the manufacturer-recommended conditions ...
-
bioRxiv - Molecular Biology 2024Quote: The glycosylation status of the proteins was studied using the PNGase-F and the EndoH (NEB) glycosidases according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The 16S rRNA gene spanning variable regions V3+V4 was amplified using the broad-range forward primer 341F: CCTAYGGGRBGCASCAG and the reverse primer 806R: GGACTACNNGGGTATCTAAT using the Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program consisted of (1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... anti-HA (catalog # 3724S, NEB), anti-PRG-1 (33) ...
-
bioRxiv - Molecular Biology 2024Quote: ... libraries were prepared by digesting the hairpins on both adapters with USER enzyme (NEB, M5505L) and PCR amplified for 16 cycles using TruSeq index adapters ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs and 200ng of RNA was converted to complementary DNA (cDNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... Q5® High-Fidelity DNA Polymerase and its recommended reaction buffer (NEB) were utilized for the amplification process.
-
bioRxiv - Molecular Biology 2024Quote: ... PADIT-seq reporter libraries were first linearized with DrdI (NEB), which cuts a 12-bp DNA sequence (GACNNNN/NNGTC ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PAGFP-TIRR plasmid was generated by amplifying a PAGFP fragment from a pPAGFP-C1 plasmid and a backbone containing TIRR using Q5® High-Fidelity DNA Polymerase and associated Q5® Reaction Buffer and GC enhancer (New England Biolabs). The PAGFP fragment was inserted C-terminally to TIRR.
-
bioRxiv - Molecular Biology 2024Quote: All cloning was performed using Gibson cloning with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The PAGFP-TIRR plasmid was generated by amplifying a PAGFP fragment from a pPAGFP-C1 plasmid and a backbone containing TIRR using Q5® High-Fidelity DNA Polymerase and associated Q5® Reaction Buffer and GC enhancer (New England Biolabs) ...