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Citations for New England Biolabs :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cells (New England Biolabs) grown to OD600 ≈ 0.5 and induced with 0.5 mM IPTG for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: PCR amplification of the insert was done with Q5 DNA polymerase from NEB. The standard Q5 reaction contained 5 μl of 5x Q5 reaction buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and XhoI (New England Biolabs, R0146) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... To do this 2.5 uL of cutsmart buffer was added to 20 uL of purified PCR product and then 1 uL of DpnI (NEB) was added ...
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bioRxiv - Molecular Biology 2024Quote: The golden gate assembly reaction was assembled in a PCR tube by mixing 100 ng of the vector with a 3 fold molar excess of the purified PCR product (calculated using the NEB bio calculator ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit from Illumina (NEB, E7770) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µL 10x Nuclease P1 buffer (NEB) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEB MultiplexOligo sets I/II/III/IV (NewEngland Biolabs, E7500L) without size selection according to the manufacturer’s protocol with modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... and purification with a PCR clean-up kit (NEB) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl of BsaI-HFv2 and 1 μl of T4 DNA ligase (NEB).
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bioRxiv - Molecular Biology 2024Quote: The strains used in this work were T7 express and shuffle T7 express Escherichia coli cells (New England Biolabs). All strains were grown in Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2024Quote: ... Both ends of the 19-mer of the 200 bp 601 DNA were labeled with biotin by Klenow fragment (NEB) with biotin-14-dATP (58) ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB). Following transformation and overnight growth on Luria Broth (LB ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF256, cTF257) and 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (NEB, C30131). 10 mL overnight cultures were diluted into 1 mL LB medium with 50 µg/mL kanamycin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... End prep and adaptor ligation was performed using the Enzymatic Methyl-seq kit (NEB). Conversion was performed identically to the amplicons ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 μL of T7 Ligase (New England Biolabs, M0318S), 0.4 μL of Fermentas FD Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for DNA fragments were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Molecular Biology 2024Quote: ... re-digestion with BamHI (NEB) and purification with a PCR clean-up kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... purified DNA fragments were subjected to library preparation with NEB Next Ultra II (NewEngland Biolabs, E7645L) and NEB MultiplexOligo sets I/II/III/IV (NewEngland Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.5 µL 10x CutSmart buffer (NEB) were added and incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... template was generated from pCRII-TOPO-NEAT1_1 (kind gift from Archa Fox) by double digestion with BamHI/NcoI (NEB), PCR amplification using Phusion polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for small DNA fragments (25-75 bp) were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Coli UDG (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... 25ul ChIP DNA or 10ng Input DNA were used to generate libraries using the NEBNext Ultra II Library Prep Kit for Illumina (NEB). Reactions were scaled down to half otherwise processing was according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The repair template was assembled and integrated into pCC1 using Gibson assembly (Gibson et al., 2008) (NEB Hifi assembly kit). Colony PCR was used to check the plasmids had the correct inserts (F-93/R-91 ...
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bioRxiv - Molecular Biology 2024Quote: ... for each guide a pair of primers were designed which when annealed produce the guide sequence with overhangs matching those produced when pDC2 was digested with bbsI (New England Biolabs, USA), allowing for ligation to occur ...
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bioRxiv - Neuroscience 2024Quote: ... Ligated oligonucleotides were then assembled with pLV-U6-sgRNA using BsmBI (New England Biolabs) and T7 ligase (Thermo) ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... C terminal and αPKC binding region) or PICK1 (BAR domain) were made with NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Mutations of PICK1 (KD-AA and 5K-E ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA template and LUNA master mix (NEB) were added into white PCR reaction tubes and placed in a CFX Maestro (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Microbiology 2024Quote: ... The purified products were quantified using the NEBNext Library Quant kit for Illumina (NEB) and sequenced using a mid-output reagent cartridge with 2×150 paired-end reads on an Illumina Miniseq platform ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (NEB, Cat. No. C3020K). We performed 4 electroporation reactions for a final count of ∼300,000 CFUs per library and plated transformed cells on LB+ampicillin plates ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed and RNA purified using the Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s instructions except that the on-column DNase treatment was omitted ...
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bioRxiv - Microbiology 2024Quote: ... and purified using the Monarch RNA Cleanup Kit (50 μg) (NEB) and eluted in RNase-free water ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Neuroscience 2024Quote: ... by Gibson assembly (New England Biolabs) using the following primers ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... Mutant segment 7 plasmids were generated through Q5 Site-Directed Mutagenesis Kit (New England BioLabs).