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Citations for New England Biolabs :
1051 - 1100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... following linearization of the vector through restriction enzyme digestion using AgeI-HF (NEB) and SalI-HF (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was generated using the ProtoScript II Reverse Transcription Kit (New England BioLabs). qPCR was performed on a LightCycler 480 real time PCR system (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µM FeSO4 and 1 µl murine RNase inhibitor (NEB). The products of the reactions were digested to nucleosides and analyzed by LC-MS/MS ...
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bioRxiv - Neuroscience 2024Quote: ... and SalI-HF (NEB). Cloning was done using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... 2μL of RNase 1f (New England Biolabs) was added to samples and incubated at room temperature for 45 minutes under gentle agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... then the two halves collected in 200 µL of 1x RNA/DNA protection buffer (NEB #T2010S) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA from single embryonic halves was extracted using Monarch Total RNA Miniprep Kit (NEB #T2010S), following manufacturer’s instructions for mammalian whole blood with some modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... and introduced to pCS2+ vector using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621). The H3-tail sequence (Shindo & Amodeo ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by amplification with Q5 polymerase (NEB). These cDNAs were subsequently cloned into plasmids for cytoSUS using a Gibson approach96 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... high-fidelity PCR reactions (NEB Q5 DNA Polymerase) with pBSV2G and pcon++flgV were performed using primers PA470 + PA467 and PA466 + PA467 ...
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bioRxiv - Microbiology 2024Quote: ... using Gibson assembly (New England Biolabs, E2611S) and transformed into DH5α E ...
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bioRxiv - Genetics 2024Quote: ... DNA was PCR amplified with Q5 high fidelity DNA polymerase (NEB). The forward primer MitfaPCR-F binds to the mitfa genomic region upstream of the insertion site and the reverse primer 5’homology_amplify-R binds the p2A region of the insertion cassette ...
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bioRxiv - Genetics 2024Quote: ... gcry1:BFP vector backbone was assembled with the 2A and nCas9n PCR amplicons using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB # E5520S) following the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... and 400 U/µL of T4 DNA ligase (New England Biolabs M0202S). We observed that either 0.5:1 or 1:1 molar ratios of insert:backbone were optimal for library uniformity ...
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bioRxiv - Genomics 2024Quote: ... All arrayed cloning was performed in NEB Stable chemically competent cells (New England Biolabs C3040H) according to the manufacturer’s recommendations and all cultures were grown at 30 °C ...
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bioRxiv - Genomics 2024Quote: ... Entry vectors were digested with BsmBI-v2 (New England Biolabs R0739L), dephosphorylated with rSAP (New England Biolabs M0371L) ...
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bioRxiv - Genomics 2024Quote: ... of entry vector and 2 ng of gene fragment were assembled in a 2.5 µL reaction of 1X T4 DNA ligase buffer (New England Biolabs B0202S) and 1X BsmBI-v2 Golden Gate Enzyme Mix (New England Biolabs E1602S ...
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bioRxiv - Genomics 2024Quote: ... Amplified oligo libraries were digested with BsmBI-v2 (New England Biolabs R0739L) and purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI; NEB catalog #R0146). The digested genomic DNA was resolved on a 1% ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were then constructed using the NEBNext Ultra II RNA Library prep for Illumina kit (NEB#E7760L) NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... the libraries were firstly constructed using the NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB). Total RNA was then ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase ...
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bioRxiv - Molecular Biology 2024Quote: ... The hairpins within the adaptors were digested with USER (NEB, catalog #M5505), and the DNA was amplified with Illumina TruSeq primers and barcodes ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA-seq libraries were constructed using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM β-ME) supplied with protease inhibitors and an RNase inhibitor (NEB, M0314L) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μg of venom in 10 µL of reducing SDS-PAGE buffer (6X stock solution, NEB B7024S with 30% β-mercaptoethanol) was run per lane on BioRad™ Mini PROTEAN pre-cast TGX acrylamide gels (15 well ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA was treated with RNase H (NEB, M0297S) (5 U per µg of gDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM vanadyl-ribonucleoside (VRC; #S1402S, New England Biolabs) overnight at 37°C ...
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bioRxiv - Genetics 2024Quote: GSTag or GSTag-FUZ were transformed into BL21 competent cells (NEB) and the bacteria cells were treated with IPTG for protein induction for 6 hours at 30°C ...
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bioRxiv - Microbiology 2024Quote: Full length sialidase genes were amplified (Q5 polymerase, New England Biolabs, M0492S) from purified genomic DNA (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were cooled and treated with NruI (New England Biolabs) to facilitate analysis of recombination products ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, Massachusetts, USA), 0.5 µL of template DNA/cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and T4 PNK (New England Biolabs). IP samples underwent on-bead ligation of barcoded RNA adapters (/5phos/rArGrArUrCrGrGrArArGrArGrCrGrUrCrGrUrG/3SpC3/ ...
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bioRxiv - Molecular Biology 2024Quote: ... IP samples were washed and dephosphorylated with FastAP (New England Biolabs, Ipswich, MA, USA) and T4 PNK (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Library was prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB) and SYBR Green I (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... digested with DpnII overnight (NEB, Cat# R0543S) and subjected to proximity ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... including digestion with HaeIII (NEB, Cat# R0108L), end-plus-A treatment ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 µL of OneTaq polymerase (New England Biolabs) and 9 µL water ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was digested by EagI (New England Biolabs) and ligated into FMR1 RNA construct backbone instead of 5’UTR of FMR1 sequence using T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... and DNA end-repaired with 1X T4 DNA ligase buffer (New England Biolabs), 0.4 mM dNTP mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Dephosphorylation of DNA ends was performed by addition of 5 μl rSAP (NEB, #M0203) and incubation at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Igepal CA-630) and incubated with 30 μl of amylose resin (E8021S, NEB), pre-equilibrated in buffer I ...
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bioRxiv - Molecular Biology 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the Luna universal qPCR master mix (NEB, M3003X). Gene expression levels were normalized to housekeeping genes EF1 ...
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bioRxiv - Genomics 2024Quote: ... MboI restriction enzyme (NEB, R0147M) was added and chromatin was digested at 37°C for 5 hr ...
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bioRxiv - Genomics 2024Quote: ... and Ligation Module (NEB, E7445L) following the operation manual ...
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...