Labshake search
Citations for New England Biolabs :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was treated with RNA 5′ pyrophosphohydrolase (New England Biolabs) for 2 h at 37℃ ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.3 μl of T4 DNA ligase I (NEB, M0202L), 6.25-µl of double-stranded barcoded oligonucleotides (1st set of sub-barcodes ...
-
bioRxiv - Molecular Biology 2024Quote: RNA was extracted using the Monarch Total RNA miniprep kit (New England Biotechnologies (NEB) #T2010 ...
-
bioRxiv - Molecular Biology 2024Quote: ... were generated by extending a common sgRNA(F+E) sequence with gene-specific crRNA sequence downstream of a T7 promoter by PCR with Phusion polymerase (NEB). PCR product was gel extracted and sgRNA was in vitro transcribed using the MegaShortScript™ T7 Transcription Kit (Invitrogen AM1354 ...
-
bioRxiv - Genomics 2024Quote: ... 0.2X of 2X Instant Sticky-end Ligase Master Mix (NEB, M0370), 0.8X of 5X Quick Ligase Buffer (NEB ...
-
bioRxiv - Genomics 2024Quote: ... 200 µL of 1X rCutSmart buffer (NEB, B7204S) containing 0.25% SDS was used to thoroughly resuspend and permeabilize the nuclei and incubated at 62°C for 10 min using Eppendorf Thermomixer C (Eppendorf) ...
-
bioRxiv - Genomics 2024Quote: ... 0.8X of 5X Quick Ligase Buffer (NEB, B6058S), 6% (vol/vol ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10,000 copies of pBR322 (4361 bp) (New England Biolabs, MA, USA)) (31) ...
-
bioRxiv - Molecular Biology 2024Quote: ... the linear DNA was digested using 28.8 U/μL of plasmid-safe ATP-dependent DNase Exonuclease V (ExoV, RecBCD) (New England Biolabs, MA, USA) for 1 h at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNAse (100 μg/ml) at room temperature for 15 min and then with proteinase K (825 μg/ml, NEB™, P8107S) for 1 hour at 65°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... All PCR amplification for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
-
bioRxiv - Molecular Biology 2024Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
-
bioRxiv - Molecular Biology 2024Quote: ... The libraries were then prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, MA, USA) in accordance with the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... Then 3 μl USER Enzyme (NEB, USA) was used with size-selected ...
-
bioRxiv - Immunology 2024Quote: ... The region after the TRR to the middle of exon 1 was amplified from 50 ng of gDNA using Phusion® High-Fidelity polymerase (New England Biolabs, M0530) according to the manufacturer’s instructions with the following primers ...
-
bioRxiv - Immunology 2024Quote: ... mRNA was purified from 4 µg of total RNA with a magnetic mRNA isolation kit (New England Biolabs, S1550S). cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs, M0253S) primed with random hexamers (Life Technologies ...
-
bioRxiv - Immunology 2024Quote: ... after adding an A-overhang with Taq DNA polymerase (New England Biolabs, M0273). Positive clones were selected and sequenced at the UC Berkeley Sequencing Facility.
-
bioRxiv - Molecular Biology 2024Quote: ... mutagenised amplicons were digested with DpnI (New England Biolabs (NEB)) to remove template DNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mutations and deletions were introduced by site-directed PCR mutagenesis (TRF1, TRF2, BLM) or during artificial DNA synthesis (Top3A) and cloned by the recombination-based HiFi assembly strategy (NEB). Amino acid positions in each protein correspond to the indicated reference sequences.
-
Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... Illumina sequencing adaptors were ligated onto the amplicons using the NEBNext Adaptor kit for Illumina (NEB). The amplicons were then pooled ...
-
Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... Tiled amplicons were amplified using Q5 Hot Start polymerase (NEB) and the pooled primers described in ARTIC3 (Quick 2020 ...
-
bioRxiv - Molecular Biology 2024Quote: ... the library for sequencing was constructed with FS DNA Library Prep Kit (NEB, E7805) by following the manual instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The addition of poly(A) tails to mRNA transcripts was performed using the Poly(A) Polymerase (New England BioLabs, UK) and included the addition of RNase inhibitor (New England BioLabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... the wildtype 3xFlag-Ptchd1-pcDNA3.1 vector was amplified using Q5 high-fidelity DNA polymerase (NEB; Ipswich, MA), with the forward primer containing the mutant codon of interest ...
-
bioRxiv - Molecular Biology 2024Quote: ... ABCA4 missense variants were introduced in WT-ABCA4-SmBiT by site-directed mutagenesis using Q5 Site-directed mutagenesis kit quick protocol (NEB, E0554) according to the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2024Quote: ... coli cells (#C2988, NEB) or NEB 5-alpha Competent E.coli (High Efficiency ...
-
bioRxiv - Molecular Biology 2024Quote: ... 250 pmol of each RNA oligo was treated with 10 units calf intestinal alkaline phosphatase (CIP, New England Biolabs Inc., M0290S) in 37°C for 30 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... Robust synthesis of expected RNA product was comparable to commercially available T7 RNA polymerase (NEB).
-
bioRxiv - Molecular Biology 2024Quote: cDNA libraries for sequencing were constructed using TruSeq RNA Library Prep Kits for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... and assembled using Gibson Assembly Cloning Kit (NEB, E5510S) according to manufacturer instructions ...
-
Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
-
bioRxiv - Genomics 2024Quote: ... was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Genomics 2024Quote: ... coli cells (NEB), which were spread on two 150-mm LB-agar plates containing 100 µg/ml ampicillin and incubated overnight at 37°C ...
-
bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
-
bioRxiv - Genomics 2024Quote: ... and the library preparation was conducted using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed on the NextSeq500 machine (Illumina) ...
-
bioRxiv - Genomics 2024Quote: ... tissue samples were treated with 0.05% (v/v) proteinase K (New England Biolabs [NEB]; P8107S) in pre-warmed (37 °C ...
-
bioRxiv - Genomics 2024Quote: ... tissue samples were treated with 0.05% (v/v) proteinase K (New England Biolabs [NEB]; P8107S) in pre-warmed (37 °C ...
-
bioRxiv - Genomics 2024Quote: ... Samples were then rinsed with 2X SSC before incubating with a blocking buffer (10% BSA, 3% v/v 6% v/v murine RNase inhibitor [NEB, M0314L] in 2X SSC) for 30 minutes ...
-
bioRxiv - Genomics 2024Quote: ... coli (NEB C3020K). The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The extracted nucleic acids were fragmented using a restriction enzyme cocktail with BsrB I (NEB, R0102S), HindIII (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: qRT-PCR was conducted by using Luna Universal qPCR Master Mix (New Englad Biolabs) and a CFX ConnectTM Real-Time System (Bio-Rad Laboratories ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were washed three times with 1× PBS and incubated with or without 60 U/mL RNase H (M0297S, NEB) at 37°C for 36 h or left untreated ...
-
bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
-
bioRxiv - Molecular Biology 2024Quote: ... and amplified using high-fidelity 2X PCR Master Mix (New England Biolabs) using primers with standard ATAC-seq barcodes (Buenrostro et al. ...
-
bioRxiv - Genomics 2024Quote: Libraries were prepared using the NEBNext DNA Modules Products (New England Biolabs, Ipswich, MA, USA) with an ‘on bead’ protocol developed by Genoscope ...
-
bioRxiv - Genomics 2024Quote: ... were then added using the NEBNext Sample Reagent Set (New England Biolabs, Ipswich, MA, USA) and purified using Ampure XP (Beckmann Coulter Genomics ...
-
bioRxiv - Genomics 2024Quote: ... or using the NEBNext Ultra II Directional RNA Library Prep for Illumina (New England BioLabs) according to the manufacturer’s protocol ...