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Citations for New England Biolabs :
1251 - 1300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... After CIP inactivation (2 min at 80°C) piRNAs were 5΄end radiolabeled by T4 PNK (NEB) with [γ-32P] ATP (10mCi/mL ...
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bioRxiv - Molecular Biology 2024Quote: SINV was produced from pT7-SVwt plasmid90 that was first linearized with XhoI and purified to use it as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (NEB). Transcribed viral RNA was transfected into BHK-21 using Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB) followed by Illumina 150 Paired-End sequencing in biological replicates ...
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bioRxiv - Molecular Biology 2024Quote: ... polyadenylated RNA was purified with oligo(dT) beads (NEB) from 15 µg of total RNA extracted from P24 testes ...
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bioRxiv - Microbiology 2024Quote: ... The HIV-1AC-1 mutations were introduced into WT and N74D pNLdE-luc using the Gibson Assembly Cloning kit (New England Biolabs) and primers shown in Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... from HIV-1 infected cells was digested and ligated with linkers using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and its associated protocol ...
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bioRxiv - Microbiology 2024Quote: ... A repair template plasmid carrying the ∼1 kb of sequence immediately 5’ and 3’ to this gRNA sequence from the H99 genome was constructed using HiFi DNA Master Mix (NEB Cat#E2621) with a standard HYGR cassette inserted between the two flanks ...
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bioRxiv - Microbiology 2024Quote: ... The SV40 NLS was added to the C-terminus of CypA via annealing partially complimentary primers encoding the SV40 NLS (Table S1) at 20 μM with NEB buffer 2 (New England Biolabs) at 95° C for 4 min and 70° C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were used to constructed libraries using NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: Mouse Hapln1 (NM_013500) was cloned into the pAAV vector by PCR with the following primers and ligase (NEB) or In-Fusion cloning (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were washed three times for 5’ each with PBS-0.01%Tween and incubated with 0.24 mg/ml Monarch RNase A (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Strand-specific RNA-seq libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® sequencing (E7760S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Both the full length LINC01918_201 and the pcDNA3.1 empty vector (a kind gift from Prof. Chris Marine) were digested with BamHI-HF (NEB) and EcoRI-HF (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and EcoRI-HF (NEB) were added to the full-length transcript at the 5’ end and 3’ end respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... The linearized vector was then ligated to the digested PCR product using a quick ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into SfiI digested backbone from pCH117 using T4 DNA ligase (New England Biolabs). All promoter variants were verified by Sanger sequencing (Elim Biopharmaceutics Inc).
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bioRxiv - Molecular Biology 2024Quote: ... recognition sites for the restriction enzymes BamHI-HF (NEB) and EcoRI-HF (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of DNA polymerase I (10 U/μL, NEB) and 0.5 μL RNase H (2 U/μL ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin was digested with 800U DpnII (NEB), and digested ends were marked with biotin (ref ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry fluorescent protein gene was inserted into pHERD20T vector according to the manufacturing protocol of NEBuilder® HiFi DNA Assembly kit (New England Biolabs, UK). The resulting plasmid was referred to as pHERDmCh ...
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bioRxiv - Molecular Biology 2024Quote: ... EM-seq libraries were prepared with the NEBNext® Enzymatic Methyl-seq kit (E7120S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were treated with 0.24mg/ml Monarch RNase A (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was labeled by fill-in of EcoRI overhangs with the DNA polymerase I Klenow fragment (New England Biolabs) in the presence of [α-32P] dATP (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Immunology 2024Quote: ... AscI (for U6 fragment) (NEB, #R0558), or SbfI-HF (for crRNA fragment ...
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bioRxiv - Immunology 2024Quote: ... BsaI-HF (NEB, #R3535/R3733), AscI (for U6 fragment ...
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bioRxiv - Immunology 2024Quote: ... and digested using DpnI (NEB, #R0176S), BsaI-HF (NEB ...
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bioRxiv - Immunology 2024Quote: ... Coli (NEB, #C3040) at 42°C and the ligation products were spread onto ampicillin selection plates ...
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bioRxiv - Immunology 2024Quote: ... or SbfI-HF (for crRNA fragment) (NEB, #R3642). pLenti backbone was cut with AscI/SbfI-HF and purified using the QIAquick PCR purification kit ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Microbiology 2024Quote: ... an inverse PCR was performed using R3-p21 and the oligonucleotides ToANV-rectest-For/ToANV-rectest-Rev (Supplementary Table 1) using Phusion High-Fidelity DNA Polymerase (New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... two inverse PCRs were carried out using R3-p21 as a template and oligonucleotides Trip.A.For/Trip.A.Rev and Trip.B.For/Trip.B.Rev (Supplementary Table 1) using Phusion High-Fidelity DNA Polymerase (New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Neuroscience 2024Quote: ... Phusion High-Fidelity DNA Polymerase (cat# M0530L, New England Biolabs, MA, USA), and Sanger sequencing at Massey Genome Service ...
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bioRxiv - Neuroscience 2024Quote: ... whereas Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). All sequences were verified using Sanger sequencing (Microsynth).
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... in 1x CutSmart buffer (NEB). In vitro transcription was performed by adding linearized plasmid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by processing with the NEBNext Ultra Directional RNA Sequencing for Illumina kit (New England Biolabs). Illumina sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using polyA-selection followed by NEBNext Ultra Directional RNA library preparation protocol (New England Biolabs). Sequencing was performed on Illumina HiSeq 2500 instrument ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (1.2 μg) was treated with 2 U RNase H (NEB, M2097) per µg of gDNA for 4 h at 37°C ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and then treated with 5.5 U RNase H (NEB, M0297) per µg of DNA overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA extraction was performed using the Monarch total RNA Miniprep Kit (NEB, T2010S) following the manufacture recommendations and RNA samples were stored at −80°C until use.
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bioRxiv - Molecular Biology 2024Quote: ... The purification protocol for the poly(A)+ RNA was optimized from the manufacturer (NEB) as ribosomes have very high content of rRNAs ...
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bioRxiv - Molecular Biology 2024Quote: NEB Next® Poly(A)+ mRNA Magnetic Isolation Module (New England BioLabs Inc.) was used to purify mRNA based upon on the coupling of oligo d(T)25 to 1 μm paramagnetic beads which were then used as the solid support for the direct binding of poly(A)+ RNA ...
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bioRxiv - Neuroscience 2024Quote: ... Inserts were cloned into KpnI– and AgeI-digested AAV backbones using HiFi DNA Assembly Master Mix (NEB). All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Eluted RNA was treated with DNase (NEB) in the following reaction mixture ...
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bioRxiv - Microbiology 2024Quote: ... Fragmented DNA was end-repaired (NEB #E6050) and purified on Ampure SPRIselect beads (Beckman-Coulter #B23317) ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with medium range primers 24 using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...