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Citations for New England Biolabs :
1101 - 1150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ligation (T4 DNA ligase, NEB). The resulting saci_1116 expression plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragment was cloned into pSVAaraFX-saci_1116-CtSS by restriction with SacII and NcoI (NEB) followed by ligation (T4 DNA ligase ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Half of the fragmented nucleic acids were digested with RNase H (New England Biolabs) overnight at 37°C to serve as a negative control ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Xba I (NEB, R0145L), and EcoRI (NEB ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and EcoRI (NEB, R3101L) overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... The CRISPR-targeted sequences were subsequently amplified using Phusion® High-Fidelity DNA Polymerase (NEB). The primers used for the PCR were as follows ...
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bioRxiv - Genomics 2024Quote: ... was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
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bioRxiv - Genomics 2024Quote: ... was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2024Quote: ... coli cells (NEB), which were spread on two 150-mm LB-agar plates containing 100 µg/ml ampicillin and incubated overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
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bioRxiv - Genomics 2024Quote: ... and the library preparation was conducted using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed on the NextSeq500 machine (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... tissue samples were treated with 0.05% (v/v) proteinase K (New England Biolabs [NEB]; P8107S) in pre-warmed (37 °C ...
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bioRxiv - Genomics 2024Quote: ... tissue samples were treated with 0.05% (v/v) proteinase K (New England Biolabs [NEB]; P8107S) in pre-warmed (37 °C ...
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bioRxiv - Genomics 2024Quote: ... Samples were then rinsed with 2X SSC before incubating with a blocking buffer (10% BSA, 3% v/v 6% v/v murine RNase inhibitor [NEB, M0314L] in 2X SSC) for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... coli (NEB C3020K). The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The extracted nucleic acids were fragmented using a restriction enzyme cocktail with BsrB I (NEB, R0102S), HindIII (NEB ...
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bioRxiv - Molecular Biology 2024Quote: qRT-PCR was conducted by using Luna Universal qPCR Master Mix (New Englad Biolabs) and a CFX ConnectTM Real-Time System (Bio-Rad Laboratories ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were washed three times with 1× PBS and incubated with or without 60 U/mL RNase H (M0297S, NEB) at 37°C for 36 h or left untreated ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... and amplified using high-fidelity 2X PCR Master Mix (New England Biolabs) using primers with standard ATAC-seq barcodes (Buenrostro et al. ...
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bioRxiv - Genomics 2024Quote: Libraries were prepared using the NEBNext DNA Modules Products (New England Biolabs, Ipswich, MA, USA) with an ‘on bead’ protocol developed by Genoscope ...
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bioRxiv - Genomics 2024Quote: ... were then added using the NEBNext Sample Reagent Set (New England Biolabs, Ipswich, MA, USA) and purified using Ampure XP (Beckmann Coulter Genomics ...
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bioRxiv - Genomics 2024Quote: ... or using the NEBNext Ultra II Directional RNA Library Prep for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B- 10). Libraries were then indexed and PCR amplified (10 cycles ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and BglII (NEB, R0144L). Linker oligonucleotides ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (10 µg) was digested overnight at 37°C with 100 U each of the restriction endonucleases MseI (NEB, R0525L) and BglII (NEB ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 800 U T4 DNA ligase (NEB, M0202S). Viral LTR–host DNA junctions were amplified using semi-nested PCR with a unique linker-specific primer and LTR primers ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM β-ME) supplied with protease inhibitors and an RNase inhibitor (NEB, M0314L) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μg of venom in 10 µL of reducing SDS-PAGE buffer (6X stock solution, NEB B7024S with 30% β-mercaptoethanol) was run per lane on BioRad™ Mini PROTEAN pre-cast TGX acrylamide gels (15 well ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA was treated with RNase H (NEB, M0297S) (5 U per µg of gDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM vanadyl-ribonucleoside (VRC; #S1402S, New England Biolabs) overnight at 37°C ...
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bioRxiv - Genetics 2024Quote: GSTag or GSTag-FUZ were transformed into BL21 competent cells (NEB) and the bacteria cells were treated with IPTG for protein induction for 6 hours at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were generated at the Novogene Bioinformatics Institute using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2024Quote: BL21(DE3) cells (NEB) were co-transformed with a pTarget plasmid encoding a target sequence and a T7-inducible IS621 recombinase and a pDonor plasmid encoding a bridge RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... with the addition of 20 U of Murine Rnase Inhibitor (New England Biolabs). qPCR reactions were run in 12 μl with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
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bioRxiv - Molecular Biology 2024Quote: ... were generated by extending a common sgRNA(F+E) sequence with gene-specific crRNA sequence downstream of a T7 promoter by PCR with Phusion polymerase (NEB). PCR product was gel extracted and sgRNA was in vitro transcribed using the MegaShortScript™ T7 Transcription Kit (Invitrogen AM1354 ...
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bioRxiv - Genomics 2024Quote: ... 0.2X of 2X Instant Sticky-end Ligase Master Mix (NEB, M0370), 0.8X of 5X Quick Ligase Buffer (NEB ...
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bioRxiv - Genomics 2024Quote: ... 200 µL of 1X rCutSmart buffer (NEB, B7204S) containing 0.25% SDS was used to thoroughly resuspend and permeabilize the nuclei and incubated at 62°C for 10 min using Eppendorf Thermomixer C (Eppendorf) ...
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bioRxiv - Genomics 2024Quote: ... 0.8X of 5X Quick Ligase Buffer (NEB, B6058S), 6% (vol/vol ...
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bioRxiv - Molecular Biology 2024Quote: ... 10,000 copies of pBR322 (4361 bp) (New England Biolabs, MA, USA)) (31) ...
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bioRxiv - Molecular Biology 2024Quote: ... the linear DNA was digested using 28.8 U/μL of plasmid-safe ATP-dependent DNase Exonuclease V (ExoV, RecBCD) (New England Biolabs, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...