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Citations for New England Biolabs :
1301 - 1350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... truncated KQ (NEB). Excess adapters were digested using 5’-Deadenylase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The products were finally purified with Monarch RNA Cleanup Kit (NEB). Sequences of RNA oligonucleotides can be found in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... and RecJf (NEB). For BACS ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μl 4× Template Switching RT buffer (NEB), 1 µl of 75 μM T7-TSO (5’-/5Biosg/ACTCTAATACGACTCACTATAGGGAGAGGGCrGrGrG-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation was performed in 25% PEG 8000 (61) by T4 RNA Ligase 2 Truncated K227Q (NEB) for 8 h at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: The untreated and treated RNA were digested into nucleosides by Nucleoside Digestion Mix (NEB) in a 50 µl solution according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl RNase H (NEB), and 70 μl nuclease-free H2O to the cDNA mixture and incubation at 37 °C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the whole lysate was incubated with pre-equilibrated oligo(dT)25 magnetic beads (NEB) for 1 h at 4 °C with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... The 72mer Ψ-containing model RNA used for mutation analysis and the 1.8-kb 10% Ψ-modified RNA used for UHPLC-MS/MS were prepared by T7 in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB) and Pseudo-UTP (Jena Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... After CIP inactivation (2 min at 80°C) piRNAs were 5΄end radiolabeled by T4 PNK (NEB) with [γ-32P] ATP (10mCi/mL ...
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bioRxiv - Molecular Biology 2024Quote: SINV was produced from pT7-SVwt plasmid90 that was first linearized with XhoI and purified to use it as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (NEB). Transcribed viral RNA was transfected into BHK-21 using Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB) followed by Illumina 150 Paired-End sequencing in biological replicates ...
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bioRxiv - Molecular Biology 2024Quote: ... polyadenylated RNA was purified with oligo(dT) beads (NEB) from 15 µg of total RNA extracted from P24 testes ...
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bioRxiv - Microbiology 2024Quote: ... The HIV-1AC-1 mutations were introduced into WT and N74D pNLdE-luc using the Gibson Assembly Cloning kit (New England Biolabs) and primers shown in Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... from HIV-1 infected cells was digested and ligated with linkers using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and its associated protocol ...
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bioRxiv - Microbiology 2024Quote: ... A repair template plasmid carrying the ∼1 kb of sequence immediately 5’ and 3’ to this gRNA sequence from the H99 genome was constructed using HiFi DNA Master Mix (NEB Cat#E2621) with a standard HYGR cassette inserted between the two flanks ...
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bioRxiv - Microbiology 2024Quote: ... The SV40 NLS was added to the C-terminus of CypA via annealing partially complimentary primers encoding the SV40 NLS (Table S1) at 20 μM with NEB buffer 2 (New England Biolabs) at 95° C for 4 min and 70° C for 10 min ...
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A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integrationbioRxiv - Molecular Biology 2024Quote: ... coli RNAP holoenzyme (New England Biolabs) was added to 100 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (New England BioLabs, Ref #C2527H) were transformed with the pMMHa vector encoding the C10A TTR gene ...
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bioRxiv - Molecular Biology 2024Quote: ... in accordance with manufacturer’s instructions with the addition of RNase inhibitor (New England BioLabs, UK) incorporated into each reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 μg of plasmid DNA was linearized with AhdI (New England Biolabs, UK) and extracted with phenol-chloroform for injection.
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bioRxiv - Molecular Biology 2024Quote: ... coli (New England BioLabs, Ref #C2527H) were transformed with the pET-29a vector encoding the N-terminal dual-FLAG-tagged WT TTR gene ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (New England BioLabs, Ref #C2527H) were transformed with the pMMHa vector encoding the WT TTR gene ...
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bioRxiv - Molecular Biology 2024Quote: ... and included the addition of RNase inhibitor (New England BioLabs, UK) in each reaction ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was either extracted with phenol-chloroform followed by Monarch Total RNA Miniprep kit (New England Biolabs) or with the Quick-RNA Miniprep kit (Zymo) ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA was then subjected to amplification using Q5 high-fidelity DNA polymerase (NEB) with a universal i5 primer and a uniquely barcoded i7 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1× Q5 polymerase reaction buffer (NEB) in a 20 μl reaction system.
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bioRxiv - Molecular Biology 2024Quote: ... The eluent was subsequently mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (NEB) and 1× Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... reactions were denatured with 2X RNA dye (NEB B0363S) at 95°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was treated with DNAse I (M0303, NEB) (1 U per 2.5 µg of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... mutagenised amplicons were digested with DpnI (New England Biolabs (NEB)) to remove template DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... phosphorylated by T4 polynucleotide kinase (NEB), ligated with Blunt/TA master mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... ligated with Blunt/TA master mix (NEB) or DNA ligation kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... or BamHI (NEB, Cat.R0136M) for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... A-tailing was performed by addition of 5 µL of the A-tailing mix (0.5 µL of Cutsmart buffer 10X, 0.25 µL Klenow 50 U/µL (NEB, M0212M), 0.05 µL dATP 100 mM ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was incubated in 5 mL of a clearing solution comprising Clearing Premix (Vizgen, PN 20300003) and 50 μL Proteinase K (New England BioLabs) at 37°C in a humidified benchtop incubator until the tissue was cleared.
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% (vol/vol) Triton X-100 in nuclease-free water and 1% (vol/vol) proteinase K (New England Biolabs, P8107S). The sample was digested in this buffer for ≥36h in a humidified ...
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bioRxiv - Pathology 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer recommendations ...
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bioRxiv - Pathology 2024Quote: ... according to manufacturer’s instructions) and cloned into the SacI (NEB) digested pAbAi plasmid (Takarabio) ...
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bioRxiv - Pathology 2024Quote: ... 5 min ice) and plasmids were extracted using the Monarch Plasmid Miniprep kit (NEB) following manufacturer’s instructions.
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bioRxiv - Pathology 2024Quote: ... schachtii cDNA (Q5 polymerase (NEB)) and cloned into the PCR amplified pDEST22 plasmid (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate TbGT8 5’_BSDr_TbGT8 3’_pUC19 ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼500 bp 5’ and 3’ flanking regions of Tb427.10.12290 (NEB) with primers SMD400/1 and SMD404/5 respectively using (Lister strain 427 ...
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bioRxiv - Molecular Biology 2024Quote: ... the bound TfR was digested on beads with EndoH according to the manufacturer’s recommendations (New England Biolabs). Eluates were analysed by lectin or Western blotting as described above.
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bioRxiv - Plant Biology 2024Quote: ... 2,5 μg total RNA was DNase treated according to manufacturer’s instructions (NEB, M0303, www.neb.com), precipitated in ethanol ...
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bioRxiv - Plant Biology 2024Quote: ... Luciferase RNA was then purified using Monarch RNA cleanup kit (New England Biolabs) according to Manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The presence of mutation was identified by T7 Endonuclease digestion (NEB M0302, www.neb.com). Homozygous lines were obtained for further study ...
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bioRxiv - Plant Biology 2024Quote: ... qPCRs were done using qPCR Master Mix (NEB, M3003, www.neb.com) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: A spike-in Luciferase RNA was first transcribed in vitro using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) according to Manufacturer’s instructions ...