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Citations for New England Biolabs :
951 - 1000 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (NEB, Cat. No. C3020K). We performed 4 electroporation reactions for a final count of ∼300,000 CFUs per library and plated transformed cells on LB+ampicillin plates ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed and RNA purified using the Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s instructions except that the on-column DNase treatment was omitted ...
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bioRxiv - Microbiology 2024Quote: ... and purified using the Monarch RNA Cleanup Kit (50 μg) (NEB) and eluted in RNase-free water ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Neuroscience 2024Quote: ... by Gibson assembly (New England Biolabs) using the following primers ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... Mutant segment 7 plasmids were generated through Q5 Site-Directed Mutagenesis Kit (New England BioLabs).
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bioRxiv - Molecular Biology 2024Quote: ... high concentration (NEB). The ligated cDNA was amplified with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μl 10× Template Switching RT Enzyme Mix (NEB) were added to the mixture and the reaction was incubated at 42 °C for 90 min followed by 85 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The second strand synthesis was then performed by adding 100 μl Q5 Hot Start High Fidelity Master Mix (NEB), 10 μl RNase H (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: 100 ng polyA+ RNA was annealed with 2 μl of 10 μM Oligo(dT)30VN primer (5’-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’) and 2 μl of 10 mM dNTP mix (NEB) in 12 μl solution ...
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bioRxiv - Molecular Biology 2024Quote: ... and amplified with 25 PCR cycles by Phusion High-Fidelity DNA polymerase (NEB) and adaptor specific primers carrying Illumina p5 and p7 flow cell binding sequences ...
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bioRxiv - Molecular Biology 2024Quote: ... and fragmentation (25 min at 94 °C in 2X T4 PNK reaction buffer from NEB –140 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess adapters were digested using 5’-Deadenylase (NEB) and RecJf (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess RT primers were digested using Exo I (NEB). RNA was hydrolyzed by NaOH (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragmented RNA was 3’-end repaired using T4 PNK (NEB) and ligated to RNA adapter (5’-/5rApp/AGATCGGAAGAGCGTCGTG/3SpC3/-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... in CutSmart buffer (NEB). After CIP inactivation (2 min at 80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... truncated KQ (NEB). Excess adapters were digested using 5’-Deadenylase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The products were finally purified with Monarch RNA Cleanup Kit (NEB). Sequences of RNA oligonucleotides can be found in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... and RecJf (NEB). For BACS ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μl 4× Template Switching RT buffer (NEB), 1 µl of 75 μM T7-TSO (5’-/5Biosg/ACTCTAATACGACTCACTATAGGGAGAGGGCrGrGrG-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation was performed in 25% PEG 8000 (61) by T4 RNA Ligase 2 Truncated K227Q (NEB) for 8 h at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: The untreated and treated RNA were digested into nucleosides by Nucleoside Digestion Mix (NEB) in a 50 µl solution according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl RNase H (NEB), and 70 μl nuclease-free H2O to the cDNA mixture and incubation at 37 °C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the whole lysate was incubated with pre-equilibrated oligo(dT)25 magnetic beads (NEB) for 1 h at 4 °C with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... The 72mer Ψ-containing model RNA used for mutation analysis and the 1.8-kb 10% Ψ-modified RNA used for UHPLC-MS/MS were prepared by T7 in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB) and Pseudo-UTP (Jena Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μl T4 PNK (NEB, #M0201L) to the dephosphorylated nuclei sample and incubate at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The sorted nuclei were resuspended with a digestion buffer (44 ul of 10x NEB DpnII buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin labeled nuclei were washed once with 1x NEB ligation buffer and resuspended with ligation mix (100 ul 10x NEB ligation buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 μl 5 U/μL Klenow Fragment (NEB, M0210S) and 2 μl T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Wash/rinse the nuclei with 1xNEBbuffer2.1(NEB, #B7202S) for three times ...
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bioRxiv - Genetics 2024Quote: ... and 10mM dNTP mix (NEB). All qPCR reactions were carried out on an Applied Biosystems Step One plus real-time PCR system using standard thermal-cycling conditions optimised for KAPA SYBR® FAST qPCR Mix ...
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bioRxiv - Genetics 2024Quote: ... MultiV Reverse Transcriptase (RT) enzyme (New England BioLabs; NEB), 10x RT buffer (NEB ...
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bioRxiv - Genetics 2024Quote: ... 10x RT buffer (NEB) and 10mM dNTP mix (NEB) ...
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bioRxiv - Genetics 2024Quote: ... MultiV Reverse Transcriptase (RT) enzyme (New England BioLabs; NEB), 10x RT buffer (NEB ...
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bioRxiv - Genetics 2024Quote: ... RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were treated with 10 μl of T4 Polynucleotide kinase (PNK; NEB) in 100 μl of 1X T4 PNK Reaction buffer containing 1 U/μl SUPERase•In™ RNase Inhibitor at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were pelleted and DNA integrity tested by loading DNA on an agarose gel after incubation with the N1aIII restriction enzyme (NEB, R0125L). After DNA restriction ...
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bioRxiv - Molecular Biology 2024Quote: ... and then A-tailed using Klenow (exo-) (NEB, M0212M). RNA 3’ OH ends were ligated with the 5’ rApp ends of the bridge adapter (IDT ...
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bioRxiv - Molecular Biology 2024Quote: ... Oligos (20 pmoles) were labeled at their 5’ ends using gamma-32P-rATP (3000 Ci/mmole) and polynucleotide kinase (New England Biolabs), and purified on 7M urea ...
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bioRxiv - Molecular Biology 2024Quote: ... second-strand synthesis was done using Phusion Polymerase (NEB) and a set of primers complementary to the cDNA (i ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was done using intron-specific outward pointing primers (Table S7) and Phusion DNA polymerase (NEB). Purified PCR products were incubated with Taq to add a 3’ A residue and cloned using a Topo cloning kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Then amplicon DNA were end-repaired using NEBNext Ultra II End-Repair/dA-tailing Module (NEB E7546). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5 site directed mutagenesis kit (NEB) was used to generate various SARS-CoV-2 FSE conformation stabilizing constructs and other length dependent constructs and their sequences were confirmed through sanger sequencing (Eurofins genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using NEBNext Ultra II DNA Library prep kit (New England Biolabs) following manufacturer’s instructions for H3K9ac CUT&RUN DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... then the two halves collected in 200 µL of 1x RNA/DNA protection buffer (NEB #T2010S) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA from single embryonic halves was extracted using Monarch Total RNA Miniprep Kit (NEB #T2010S), following manufacturer’s instructions for mammalian whole blood with some modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... and introduced to pCS2+ vector using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621). The H3-tail sequence (Shindo & Amodeo ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by amplification with Q5 polymerase (NEB). These cDNAs were subsequently cloned into plasmids for cytoSUS using a Gibson approach96 ...