Labshake search
Citations for New England Biolabs :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... + 2 mM vanadyl-ribonucleoside complex (NEB S1402) + 0.02% [w/v] BSA ...
-
bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped co-transcriptionally during the T7 RNA polymerase reaction by decreasing GTP to 0.125 mM with addition of 2.5 mM cap analog (G-cap, NEB S1407S; A-cap, NEB S1406S).
-
bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (T4 PNK, NEB, M0201) was used to add a phosphate group at the 5′ ends ...
-
bioRxiv - Cancer Biology 2024Quote: ... using T4 quick ligase (NEB, M2200), of sequencing adapters ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Samples were incubated at 37°C for 1h and then inactivated by addition of EDTA at a final concentration of 20mM ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA Fragments were repaired by T4 DNA polymerase (NEB, M0203), and T4 polynucleotide kinase (T4 PNK ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: ... An adenosine base was then added by Klenow fragments (NEB, M0212) to allow efficient ligation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nucleosomes labeling the following reaction was used: NEBuffer™ 2 (NEB B7202), inhibitors (as detailed above ...
-
bioRxiv - Cancer Biology 2024Quote: ... Amplicons were generated with the Q5 2x mastermix (New England Biolabs) and cloned by GoldenGate assembly into pCFD5w (Addgene 112645 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 ng of purified CUT&RUN enriched DNA was used to prepare Illumina library using the NEBNext Ultra II DNA Library Prep kit (NEB, #E76450) per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Poly(A) RNA libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7770) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... The vector was dephosphorylated using the rSAP (M0371S - NEB), loaded on a gel and the correct band was purified using Wizard SV Gel and PCR Clean-Up System ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#) ...
-
bioRxiv - Cell Biology 2024Quote: ... Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#). Libraries were pooled and sequenced on an Illumina NextSeq 550 using paired-end sequencing of 42 bases per read.
-
bioRxiv - Cell Biology 2024Quote: RNA-sequencing libraries were prepared NEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB Cat #) followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #) ...
-
bioRxiv - Cell Biology 2024Quote: ... which was amplified from human cDNA library and cloning into pEGFP-N2 using Gibson assembly method (NEB E2611L). A stop codon was inserted before EGFP in pEGFP-N2 vector to clone untagged APMAPFL ...
-
bioRxiv - Cell Biology 2024Quote: ... and prepared as previously described [Kampmann et al 2014] with modifications: Sbf1 (R3642S, NEB) was used instead of PvuII for the restriction digest ...
-
bioRxiv - Cell Biology 2024Quote: ... with T4 ligase (NEB). AOX overexpression plasmid was achieved with pCW57.1_AOX-FLAG (pCW57.1_AOX-FLAG was a gift from David Sabatini & Jessica Spinelli (Addgene plasmid # 177984 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 ug of RNA was reverse transcribed using the Lunascript Supermix (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 minutes at 50°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA libraries were amplified with multiplex oligos for Illumina sequencing (NEB, E6609S). Libraries were quantified and checked for quality using a Qubit fluorimeter and Bioanalyzer (Agilent ...
-
bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
-
bioRxiv - Cell Biology 2024Quote: Libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB). For transcription factors (BMI1 and RING1B) ...
-
bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was used to create an additional Lv165 BMI1 overexpression vectors with a P2A sequence instead of the IRES by designing primers using the New England Biolabs (NEB)-base changer website (https://nebasechanger.neb.com) in combination with the Q5 Site-Directed Mutagenesis kit (NEB, E0554S), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was used to create an additional Lv165 BMI1 overexpression vectors with a P2A sequence instead of the IRES by designing primers using the New England Biolabs (NEB)-base changer website (https://nebasechanger.neb.com ...
-
bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed with Phusion polymerase (New England Biolabs), and products were analyzed by agarose gel electrophoresis and detected using RedSafe/UV light ...
-
bioRxiv - Cell Biology 2024Quote: ... coli Poly(A) Polymerase (New England Biolabs), following the manufacturers’ protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... The RNA was capped with Vaccinia Capping System (New England Biolabs) and polyadenylated with E ...
-
bioRxiv - Cell Biology 2024Quote: MHC-ftz-Δi mRNA was synthesized in vitro using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) with MHC-ftz-Δi in pcDNA3.0 (30 ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were generated using Next Ultra II Directional RNA Library Prep kit (NEB) and ribosomal RNA depletion according to the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2024Quote: ... mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, #E7490). Poly(A ...
-
bioRxiv - Cell Biology 2024Quote: ... rRNA was depleted with a NEBNext rRNA Depletion Kit (NEB, E7400), and following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... DNA libraries were prepared using NEBNext® reactives (New England Biolabs). For MeDIP ...
-
bioRxiv - Cell Biology 2024Quote: ... orthovanadate (200 nM; New England Biolabs, P0758S) and β-glycerophosphate (25 mM ...
-
bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by library preparation using NEBNext Ultra II RNA Library Preparation Kit (NEB, E7770S). Libraries were paired-end sequenced with read lengths of 150 bp on Illumina HiSeq X Ten or Nova-seq instruments ...
-
bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and were sequenced on an Illumina NovaSeq 150bp paired-end platform ...
-
bioRxiv - Cell Biology 2024Quote: ... STAT1 proteins were dephosphorylated by lambda phosphatase (NEB, Ipswich MA) followed by the addition of protease and phosphatase inhibitors (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
-
bioRxiv - Cell Biology 2024Quote: Plasmid cloning was carried out using NEBuilder HIFI DNA assembly (NEB). Primer sequences are given in Table 1 ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA was depleted of rRNA using the Illumina rRNA Depletion Kit (NEB #E6310) and then prepared for Illumina sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina #E7760 per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, no. E7775) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... and 10mM dNTP mix (NEB). All qPCR reactions were carried out on an Applied Biosystems Step One plus real-time PCR system using standard thermal-cycling conditions optimised for KAPA SYBR® FAST qPCR Mix ...
-
bioRxiv - Genetics 2024Quote: ... MultiV Reverse Transcriptase (RT) enzyme (New England BioLabs; NEB), 10x RT buffer (NEB ...
-
bioRxiv - Genetics 2024Quote: ... 10x RT buffer (NEB) and 10mM dNTP mix (NEB) ...