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Citations for New England Biolabs :
9951 - 10000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... ECE cell extract was supplemented with micrococcal nuclease (NEB) at a final concentration of 15 u/ml and 0.75 mM of calcium chloride (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was digested in a reaction with 30 nM single-guide RNA (sgRNA, Synthego) and 30 nM spCas9 (New England BioLabs, M0386S) after pre-incubation of the reaction mix at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by 7-9 rounds of PCR amplification using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... and library preparation performed using NEBNext® FFPE DNA Repair Mix (New England Biolabs® Inc) and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (New England Biolabs® Inc) ...
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bioRxiv - Cell Biology 2023Quote: MBP-tagged RH domain was isolated via passing cleared lysate over a gravity amylose resin column containing 5 mL of settled resin (NEB). Column was washed with 150 mL of buffer before eluting protein with wash buffer supplemented with 20 mM maltose ...
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bioRxiv - Biochemistry 2023Quote: ... and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs). The IRES-containing mRNAs were uncapped and polyadenylated.
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bioRxiv - Biochemistry 2023Quote: ... The DNA fragments needed to assemble the constructs encoding GADD34Δ and K3L were PCR amplified using Q5 High-Fidelity DNA polymerase (NEB) from plasmid templates (gift of A ...
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bioRxiv - Developmental Biology 2023Quote: ... this extension could be radioactively labeled by filling in the overhangs using 32P-dCTP and Klenow enzyme (NEB). Sequences of the +-strand oligonucleotides used are described in Table 1.
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bioRxiv - Cell Biology 2023Quote: PCR amplification was carried out using a Q5® High-Fidelity DNA Polymerase kit (#M0491L, New England Biolabs, Ipswich, MA) using the following protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The digested PCR product and pSubMAAP vector was then ligated together using T4 DNA ligase (New England Biolabs, Ipsiwch, MA), transformed into Top10 competent cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Start codon of MAAP-WT2 (CTG) was substituted with ATG using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) in accordance with manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were digested using ClaI and BamHI in 1X Cutsmart buffer (New England Biolabs, Ipswich, MA) and ligated into the pCW57 backbone using T4 DNA ligase ...
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PP2A Dephosphorylates Phytochrome-Interacting Factor 3 to Modulate Photomorphogenesis in ArabidopsisbioRxiv - Developmental Biology 2023Quote: ... The supernatants were incubated with amylose resin (NEB, Cat#, E8021S) for one hour in the dark at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids and HaloTag PCR products were digested using AvrII (New England Biolabs, R0174S) and MluI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... anti-H3K9la (PTM BIOLABS, PTM1419RM, 1:1000), anti-H3K14la (PTM BIOLABS ...
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bioRxiv - Cell Biology 2023Quote: ... A second PCR was performed using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs; #M0543) and Index 1 (i7 ...
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bioRxiv - Cell Biology 2023Quote: ... and Exon 3 amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs). The following primers were used ...
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bioRxiv - Cell Biology 2023Quote: ... PCR fragments were inserted into EcoR1- and Bsu36I-digested pBID-UASC-GRM plasmid using NEBuilder HiFi DNA Assembly (New England BioLabs Inc., Cat#E5520S). Primers were designed using SnapGene and purchased from Integrated DNA Technologies ...
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bioRxiv - Genomics 2023Quote: ... We used NEBNext Ultra II library prep kit (NEB E7645) following the manufacturer’s instructions until the USER treatment step included following the adapter ligation step ...
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bioRxiv - Cell Biology 2023Quote: ... overexpression vector pT1O was used and linealized with HpaI (New England Biolabs) for subcloning ...
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bioRxiv - Genomics 2023Quote: ... with NEBNext Multiplex Oligos (96 Unique Dual Index Primer Pairs) for Illumina Barcodes (New England Biolabs, USA) and sequenced on an Illumina NovaSeq 6000 S2 150bp PE flow cell at the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies Core.
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bioRxiv - Genetics 2023Quote: ... 4 µl Luna universal qPCR Master Mix (New England Biolabs, Hitchin, UK) and 1.8 µl ultrapure water ...
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bioRxiv - Genetics 2023Quote: ... The intermediate library and G-block were column-purified and ligated (T4 DNA ligase, NEB) in three separate reactions for each subpool ...
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bioRxiv - Genetics 2023Quote: ... and degraded using Exonuclease I and III (NEB). The resulting ssDNA template was then split between 6 parallel reactions and custom mutagenic oligo primers (IDT ...
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bioRxiv - Genetics 2023Quote: ... 50 units of DpnII (NEB, #R0543M) was used for the second restriction digestion following which samples were again ligated ...
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bioRxiv - Genetics 2023Quote: ... 0.2μl 100X Bovine Serum Albumin (New England Biolabs, B9000S), 200μl 10X NEBuffer 2.1 (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Bioengineering 2023Quote: pX601 vector was digested with NcoI + BamHI (New England Biolabs, Ipswich, MA) at 37°C for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... and ligated with Quick Ligase (NEB, USA #M220S). pLKO_HOXB13_#1 (Addgene #70093 ...
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bioRxiv - Cell Biology 2023Quote: A 40 µL solution of 25% (v/v) amylose resin (NEB) was co-incubated with 500 nM MBP-RH domain and 4 µM Alexa Fluor 647-labeled RAB7A (life technologies ...
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bioRxiv - Cell Biology 2023Quote: ... and for A-tailing with Klenow Fragment (3’->5’ exo–) (NEB; M0212). A biotinylated primer (ENDseq-adaptor-1 ...
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bioRxiv - Cell Biology 2023Quote: ... the agarose plugs were dissolved with β-agarase (NEB; M0392) enzyme according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
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bioRxiv - Cell Biology 2023Quote: ... 5′-Phos-GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUU[Biotin-dT]U[Biotin-dT]UUACACTCTTTCCCTACACGACGCTCTTCCGATC∗T-3′[∗phosphorothioate bond]) was then ligated at the free DSB ends with the quick ligase enzyme (NEB; M2200). After the primer ligation ...
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bioRxiv - Cell Biology 2023Quote: ... The newly generated DSBs by the sonication step were also processed with T4 DNA polymerase (15 U) (NEB; M0203), Klenow fragment (5 U ...
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bioRxiv - Cell Biology 2023Quote: ... The libraries were then prepared by digesting the hairpin structures on both primer adaptors using Uracil-Specific Excision Reagent (USER) enzyme (NEB; M5505) and a following PCR amplification for 16 cycles with Truseq barcoded primers ...
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bioRxiv - Cell Biology 2023Quote: ... This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) to create a new HindIII site at the position encoding amino acid 569 ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using Phusion 5X Master mix (New England Biolabs GmbH, M0492S). For a 25 µl reaction ...
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bioRxiv - Genetics 2023Quote: ... The isolated ePCR products were inserted into a BsmBI-digested lentiV2-mU6-evopreQ1 vector via Gibson assembly (NEB E2621L). The assembled products were electroporated into Endura electrocompetent cells (Biosearch Technologies 60242) ...
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bioRxiv - Microbiology 2023Quote: ... the LunaScript® RT Super Mix Kit from NEB was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (NEB) according to the manufacturer guidelines ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into NEB 5-alpha F’Iq (NEB). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Microbiology 2023Quote: ... and directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). cDNA library quality was assessed using a High Sensitivity DNA Kit (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... 5’ phosphorylation of RNA fragments was achieved using T4 Polynucleotide Kinase (NEB) and Adenosine 5’-Triphosphate (ATP ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction mix was assembled using OneTaq® DNA Polymerase (M0480) (New England Biolabs, Ipswich, MA, USA) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µg of DNA was digested with DpnI (NEB; R0176) in 10 µl volume at 37°C for 16h ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by DpnII digestion in a total volume of 50 µl with 10U of DpnII (NEB; R0543). DNA was amplified by PCR with Taq polymerase using the following program ...