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Citations for New England Biolabs :
9801 - 9850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Nanopore sequencing adapters were first ligated to Cas9 cut sites by incubating 10 μl of NEBNext Quick T4 DNA Ligase (NEB), 20 μl ligation buffer (ONT) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.8 μl 10X CutSmart Buffer (NEB), 23 μl nuclease free water and 3 μl of the gRNA duplex at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... backbone by Golden Gate cloning with BsmBI (NEB, R0739) and T7 ligase (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... Then 3 µl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were constructed using the NEBuilder system (New England BioLabs, MA, USA) and enzymatic digestion and ligation (TaKaRa ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly using NEB builder HiFi DNA assembly master mix as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5α bacteria ...
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bioRxiv - Genomics 2023Quote: ... The in/out PCRs were carried out with Q5 polymerase (New England BioLabs, M0494S), while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio ...
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bioRxiv - Genomics 2023Quote: ... 10µL of transposed DNA was amplified in a 50µL PCR reaction using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541S) for 5 cycles after a 5 min elongation step ...
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bioRxiv - Biophysics 2023Quote: ... and ligated to the hairpin with short handles with T4 DNA ligase (New English Biolabs) to finish the 537-bp hairpin preparation.
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bioRxiv - Biophysics 2023Quote: ... and 1000 gel units of Micrococcal Nuclease (NEB, #M0247S) and incubated at 30 °C for 10 min under constant mixing ...
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bioRxiv - Cell Biology 2023Quote: ... 4000 units lambda phosphatase (NEB) and 1 mM MnCl2 were included in the lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... High-fidelity Phusion polymerase (New England Biolabs) was used to amplify the DNA constructs according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit polyclonal anti-SNAP-tag (Cat no. P9310S, New England Biolabs, 1:1000 dilution). Secondary antibodies used were ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... according to manufacturer’s instructions (New England Biolabs). For these samples ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs) (E6440, NEB). We used 13 min fragmentation time and 9 cycles of PCR enrichment on 1000 ng of total RNA input determined by preliminary library preparation ...
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bioRxiv - Biophysics 2023Quote: ... Derivatives of pSB963 were altered using mutagenic primers and Q5 site-directed mutagenesis kit (NEB) to generate vectors containing CEN9 ...
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bioRxiv - Biophysics 2023Quote: ... 10 μL of 10X Micrococcal Nuclease Reaction Buffer (NEB) and 1000 gel units of Micrococcal Nuclease (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Strains of Escherichia coli NEB Stable (New England Biolabs) or Rossetta (DE3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with streptavidin (1 mg/mL, New England Biolabs). The chamber was again washed with TIRF buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... for Strep-tag II pull-downs and Amylose resin (NEB) for MBP pull-downs) ...
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bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were transferred to a tube and incubated with 10 μM SNAP-Cell TMR-Star dye (New England Biolabs) for 1 hour at 4°C prior to the TEV buffer washing step ...
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bioRxiv - Genetics 2023Quote: ... Phusion HiFi DNA polymerase (New England Biolabs) was used to amplify the template DNA ...
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bioRxiv - Genetics 2023Quote: ... with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, NEB) and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs ...
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bioRxiv - Genetics 2023Quote: Illumina libraries for RNAseq were prepared using NEBNext Ultra II RNA Library Prep kit for Illumina (E7770, NEB) with NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... Quick CIP (calf-intestine alkaline phosphatase, NEB) and Cap-Clip™ Acid Pyrophosphatase (CellScript ...
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bioRxiv - Molecular Biology 2023Quote: ... M-MuLV Reverse Transcriptase (NEB, M0253L), RNase inhibitor (NEB ...
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bioRxiv - Microbiology 2023Quote: ... library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) using normalized RNA inputs per sample and without direction RNA selection ...
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bioRxiv - Microbiology 2023Quote: ... One unit of β-agarase I (New England Biolabs; Ipswich, Massachusetts, USA) and 10 µL of 10x reaction buffer was added to 100 μL of cell suspension and incubated at 42°C for 1.5 h ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), including library amplification steps ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Cancer Biology 2023Quote: ... the p27 allele and mVenus were synthesized as gBlocks (IDT) and cloned via Gibson assembly (NEB) into a modified pGIPz lentiviral expression vector (Open Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 µL Q5® High-Fidelity DNA Polymerase (New England BioLabs, Inc., M0491LVIAL), and 21.5 µL double-distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL Q5® Reaction Buffer (New England BioLabs, Inc., B9027SVIAL), 10 µL Q5® High GC Enhancer (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... power supply) with a 1kB Plus DNA Ladder (New England BioLabs, Inc., N3200L) as molecular weight marker.
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from the indicated cell lines was prepared in agarose plugs by resuspending 5 x 106 cells/ml in 0.8% agarose and digested overnight with Fse1 (NEB; R0588L) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB5α F’IQ (New England Biolabs) and selection on LB agar supplemented with 50 µg/ml Kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... Genes were PCR-amplified (New England Biolabs Q5 HotStart 2X Master Mix) from a high-titer Hammy lysate using a forward primer complementary to the first 15-25 bp of each gene sequence (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Immunology 2023Quote: ... 1000ng of total RNA was treated with the NEBNext poly (A) mRNA Magnetic Isolation Module (NEB, #E7490L). RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L) ...
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bioRxiv - Microbiology 2023Quote: ... Q5 DNA polymerase (New England Biolabs Q5 HotStart 2X Master Mix), and a high-titer Girr lysate ...