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Citations for New England Biolabs :
9701 - 9750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... 4 μL template DNA and 0.4 units of Phusion High-Fidelity DNA Polymerase (NEB) (primer sequences provided in Table S3) ...
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bioRxiv - Microbiology 2023Quote: ... Each 5 uL of the reaction contained 0.5 uL of ATP (NEB), 0.5 uL DTT (1 mM final concentration) ...
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bioRxiv - Microbiology 2023Quote: ... 0.125 uL T4 Ligase (NEB), 20 fmol CRISPRi plasmid and 100 fmol spacer duplex (0.2 uL of the working stock of the spacer duplex) ...
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bioRxiv - Microbiology 2023Quote: ... All enzymes were obtained from New England Biolabs (NEB) and oligonucleotides were received from Integrated DNA Technologies (IDT) ...
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bioRxiv - Microbiology 2023Quote: ... coli 5-alpha cells (NEB) following manufacturer’s recommendations and selected by plating on LB in the presence of appropriate antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... Reaction was performed using 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Microbiology 2023Quote: All parasite materials were centrifuged at 1,000 g for 2 min and total genomic DNA was isolated from the resulting pellets using the Monarch Genomic DNA Purification kit (New England Biolabs, Australia). For the ovine ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesized cDNA was then amplified using Phusion Hot Start Flex 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were prepared using NEBNext Ultra II DNA Library (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The phenol chloroform extracted RNA from previous step weas further purified to select for polyadenylated RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and reverse transcribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA substrates were synthesized by run off in vitro transcription (IVT) using T7 polymerase (NEB) in the presence of [α-32P]-UTP according to the manufacture’s protocol ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, #E7765L) in 96-well plates ...
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bioRxiv - Genomics 2023Quote: The RNA-Seq paired end sequencing libraries were prepared from the QC passed RNA samples using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB) as per manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: PCR reactions were performed using NEB EpiMark Hot Start Taq DNA Polymerase (New England Biolabs, catalog #M0490L). The following program was used for all bisulfite-converted genomic DNA PCRs ...
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bioRxiv - Genomics 2023Quote: Cells were lysed and RNA extracted using the Monarch Total RNA Miniprep Kit (NEB) or RNA cleanup kit (NEB ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed on genomic DNA by using Q5 High-Fidelity Taq Polymerase (New England Biolabs) followed by T7 endonuclease I assay (New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... 10 μl of PCR product was incubated with 0.5 μl of T7e1 enzyme (New England Biolabs) for 30 minutes at 37°C ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Genetics 2023Quote: ... using NEBNext multiplex oligos for Illumina (NEB E7335S). Library quality was assessed (Agilent BioAnalyzer Chip ...
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bioRxiv - Genetics 2023Quote: ... 1%BSA (NEB, catalog no. B9000S), 0.2U/µL RNase inhibitor (Ambion ...
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bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Genomics 2023Quote: ... with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample ...
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bioRxiv - Genomics 2023Quote: ... lacZ reporter gene and H11 locus homology arms using NEBuilder HiFi DNA Assembly Mix (NEB, E2621). The sequence identity of donor plasmids was verified using Nanopore sequencing (Primordium Labs) ...
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bioRxiv - Genomics 2023Quote: ... DNA was pre-amplified with 2× NEBNext Master Mix (NEB M0541S). Library amplification was assessed by qPCR on Applied Biosystems QuantStudio 3 real-time PCR system ...
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bioRxiv - Genomics 2023Quote: ... supplemented with additional 1 mM ATP (NEB P0756) at room temperature for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... The divergent promoter inserts for Gibson assembly (NEB #E2621L) were generated by digesting PDp82 with SalI and AvrII ...
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bioRxiv - Genomics 2023Quote: ... All libraries were generated with NEBNext Ultra II Directional RNA library prep kit (NEB #E7760).
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Hiscribe T7 RNA synthesis kit (NEB #E2040S). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... A PCR master mixture was prepared by combining 2,200 uL 2x NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), 22 uL common P7 primer (100 uM ...
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bioRxiv - Molecular Biology 2023Quote: ... The library was resolved on a 1% agarose gel and the smear between 300-600 bp was extracted using Monarch DNA Gel Extraction Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... 500 uL nuclei were combined with 56 uL 10 mM dNTPs (New England Biolabs) and distributed to a low-bind 96-well plate (5 uL per well) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 uL BSA (New England Biolabs) and 225 uL water were mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (NEB) and plated on Amp/IPTG/X-gal plates for blue/white screening ...
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bioRxiv - Genomics 2023Quote: Primers were used to amplify individual subpools using 25 μL 2x NEBnext PCR master mix (New England Biolabs), 2 μL of oligonucleotide pool (∼40 ng) ...
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bioRxiv - Genomics 2023Quote: ... and Pyrophosphatase (NEB M0361S), and reverse transcription (Thermo Scientific EP0751 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Molecular Biology 2023Quote: Nuclei were resuspended in 1X Second Strand Synthesis Buffer (New England Biolabs), counted with YOYO-1 Iodide and diluted to 1.3 x 105 - 2.5 x 105 per 400 uL ...
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bioRxiv - Molecular Biology 2023Quote: ... The enrichment PCR master mixture contained 2,200 OneTaq 2X Master Mix (New England Biolabs), 16.5 uL synHEK3 P7 enrichment primer (100 uM) ...
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bioRxiv - Molecular Biology 2023Quote: ... the shuttle vector was digested with BsmBI (New England Biolabs). A 87-bp region around the HEK3 gRNA target site was synthesized from IDT and amplified with a pair of primers to introduce a T7 promoter and a 16-bp barcode to its upstream and downstream ...
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bioRxiv - Molecular Biology 2023Quote: ... a T2A sequence was inserted next to P2A by NEB HiFi assembly.
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bioRxiv - Molecular Biology 2023Quote: The LT3GEN vector was purchased from Addgene (#111173) and digested with I-SceI (New England Biolabs). A fragment containing a T7 promoter and homologous sequences was ordered from IDT and assembled into the backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... DNase I treatment was performed on the column (NEB M0303S) and RNA was stored at -80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... vector was digested with XbaI and BamHI (New England Biolabs) to create a backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-15 ng of each PCR product were digested at 37 °C for 60 min with 2.5 units of XcmI (New England Biolabs) in 15 μl reactions ...