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Citations for New England Biolabs :
9901 - 9950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... WT hNEMF was generated by mutating the serine at site 86 to an arginine using Q5 site-directed mutagenesis (NEB, E0554S). pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV ...
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bioRxiv - Molecular Biology 2023Quote: ... This was combined with 1.5 µl 10X T4 DNA ligase buffer (New England Biolabs Ltd.), 1.5 µl 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µl 10 mg/ml bovine serum albumin (BSA; New England Biolabs Ltd.), 1 µl T4 DNA ligase (2000U ...
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bioRxiv - Molecular Biology 2023Quote: ... Magnetic streptavidin beads (New England Biolabs) were used to precipitate the biotinylated protein fraction ...
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bioRxiv - Microbiology 2023Quote: ... These were constructed using Gibson assembly (New England Biolabs) and employing pFE127 as a parental plasmid ...
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bioRxiv - Microbiology 2023Quote: Miniprep plasmid DNA was digested with restriction enzyme KpnI following manufacturer’s instructions (New England Biolabs). After two hours at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and M.Fnn25V for two hours at 37 °C in CutSmart buffer (NEB) containing 160 μM S-adenosylmethionine (SAM ...
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bioRxiv - Microbiology 2023Quote: ... containing 160 μM S-adenosylmethionine (SAM: NEB). This five-enzyme methylation cocktail was previously necessary to achieve genetic alteration in the strain as described in Umaña et al14.
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: ... The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610, IMG gene accessions in Table S2) and cloned using the NEBuilder HiFi DNA Assembly cloning kit (NEB E5520S). As a negative control ...
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bioRxiv - Neuroscience 2023Quote: SNAP-Surface® Alexa Fluor® 488 (NEB) were dissolve in DMSO to yield a labeling stock solution of 1 mM tags’ substrate ...
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bioRxiv - Genetics 2023Quote: ... The target loci were PCR amplified in 30 PCR cycles using Q5 High-Fidelity DNA Polymerase (New England Biolabs), locus-specific primer pairs (Supplementary Information ...
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bioRxiv - Biochemistry 2023Quote: ... or deletion of the mitochondrial targeting sequence (dMTS) of the human FAM210A coding sequence (CDS; Uniprot Entry # Q96ND0) region was amplified using Q5 DNA polymerase (NEB, Cat # M0491). The FAM210A DNA was inserted into 2Cc-T and 2CT-10 vectors using the Ligation Independent Cloning (LIC ...
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bioRxiv - Biochemistry 2023Quote: MBP-His10-FAM210A-dMTS was first bound to equilibrated amylose resin (NEB, Cat. # E8201) for 1 hour at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products and NdeI/XhoI (NEB, USA) digested pAN001 vector DNA were assembled using Gibson assembly standard protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... qPCR was performed using the Luna Universal qPCR Master Mix (NEB, USA). The PCR mixtures were cycled at 95°C for 1 min (one cycle ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Luna ® Universal One-Step RT-qPCR kit (NEB) in a CFX96 qPCR thermocycler (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was hybridised with radioactively labelled probes (T4 PNK, M0201, New England Biolabs (NEB)) in Ultrahyb buffer at 35 ᵒC and exposed to a phosphor screen that was scanned using Typhoon scanner (Cytiva).
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bioRxiv - Plant Biology 2023Quote: ... 7 µg (DNA mass) of NCPs (with 188bp NPS) or free DNA was incubated with 5 Kunitz units of MNase (NEB M0247) in a reaction buffer (30 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted using TRIzol from 14 d old seedlings and poly(A) enriched before library preparation (NEB E7490 and E7765). Libraries were sequenced on Illumina Hiseq 2500 in a 2×100 bp format ...
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bioRxiv - Biophysics 2023Quote: ... pFastBac HT 10xHis-MBP-TEV-FUS was constructed by the Gibson assembly (New England BioLabs E2611) of pFastBac HT ...
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bioRxiv - Synthetic Biology 2023Quote: ... Backbones were linearized with PCR or with restriction enzymes (NEB, 1h at 37°C). PCR-amplified or digested products were purified (Monarch PCR & DNA Cleanup Kit ...
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bioRxiv - Systems Biology 2023Quote: ... ligated to adapter (NEB, S1315S), and ribosomal RNA was removed using RiboCop (Lexogen ...
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bioRxiv - Systems Biology 2023Quote: ... Spin-purified PCR products were digested with BsaI-HF-v2 (R3733; NEB) and the size and integrity of full length and digested PCR products were confirmed on a 4% agarose e-gel (Thermo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains used were DH5α (NEB cat C2987H), E ...
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bioRxiv - Synthetic Biology 2023Quote: ... In the second Type IIS DNA assembly reaction using BbsI (New England Biolabs), these transcriptional unit constructs were assembled into the backbone plasmid pML3001 (Lebovich and Andrews 2022 ...
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bioRxiv - Synthetic Biology 2023Quote: ... in 1X T4 DNA Ligase Buffer (New England Biolabs). The reaction mixture was incubated in a thermal cycler (Bio-Rad C1000 thermal cycler ...
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bioRxiv - Synthetic Biology 2023Quote: ... were combined into a transcription unit plasmid in a Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs). The destination vector (p15A origin of replication ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 125 U T4 DNA ligase (2000 U/μl; New England Biolabs) in 1X T4 DNA Ligase Buffer (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Plant Biology 2023Quote: ... treated with 5 µg/mL Proteinase K (New England Biolabs Inc., MA, US) for 5 mins ...
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bioRxiv - Bioengineering 2023Quote: ... Five µl of the KLD product was used directly to transform chemically-competent Escherichia coli DH5ɑ cells (NEB C2988) according to the manufacturer’s protocol and plated on lysogeny broth (LB ...
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bioRxiv - Bioengineering 2023Quote: ... and the plasmids were extracted via NEB Monarch Plasmid mini-prep kit (NEB T1010). Plasmid concentrations were measured spectrophotometrically at 260 nm via a Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids were then transformed into DH5-alpha high-efficiency competent cells (NEB). Following transformation ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequencing library was generated using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA). The concentration and quality of the library was analysed with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Lysates of HEp-2/ι1hUNGs cells mock infected or infected with wild-type HSV-1(F) at an MOI of 10 for 24 h were treated with alkaline phosphatase (CIP) (New England BioLabs) as described previously48.
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bioRxiv - Microbiology 2023Quote: ... was cleaved with AfeI and BamHI endonucleases (NEB) to remove the DsRed gene ...
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bioRxiv - Microbiology 2023Quote: ... The larger fragment of the vector was gel purified as described above and the two fragments were ligated by T4 ligase (NEB) to generate the pLenti-NLuc1-9_IRES EGFP transfer plasmid ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was digested with BamHI (NEB), and the resulting 514 bp fragment was gel purified from a 1.5% Agarose gel using the GeneJet Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Point mutations were introduced using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). pET41b+_Ufd1-His6 (#117107) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutants of UFD1-His6 (deletion of amino acids 2-215) and NPL4 (T590L+F591V point mutations) 18 were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Systems Biology 2023Quote: ... and PCR master mix (NEB, M0543S). The amplification began with a 30-second denature step at 98°C ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... were derived from pUdO#a by replacing the ampicillin resistance gene with the corresponding resistance gene by two-fragment assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB E2621) according to the supplier’s protocol with the above modifications.
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bioRxiv - Physiology 2023Quote: ... sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). Two-3μg RNA per sample was used to purify mRNA using poly-T oligo attached magnetic beads and then the purified mRNA will be fragmented into short fragments (about 200 bp ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Biophysics 2023Quote: ... the annealed mixture was ligated to the dsDNA construct prepared by PCR amplification using T4 DNA ligase (New England Biolabs) at 16°C for 16 h and the ligase was heat deactivated at 65 °C for 20 min ...
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bioRxiv - Biophysics 2023Quote: ... and BamHI-HF (#R3136SVIAL, NEB) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The plasmid backbones were prepared by SfiI (NEB) restriction of the pSBtet-Hygro vector (Addgene ...