Labshake search
Citations for New England Biolabs :
9751 - 9800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 15µL Phusion HF 2X master mix (NEB #M0531L), 0.75 µL of 20 µM P3/P6 tall primer mix and 0.25 µL of 25X Sybr Green (ThermoFisher #S7563) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL 10X NEB Buffer #2 (NEB #B7002S), 5 µL RppH (NEB #M0356S) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL RppH (NEB #M0356S). Decapped RNA was cleaned using Zymo Oligo clean and concentrator kit (Zymo Research #D0460 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of T4 RNA Ligase (NEB #M0437M), 1 µL of 100 mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapters were trimmed from the fastq files using cutadapt (v 2.5) (34) before alignment with the hg38 reference genome (gencode v35) (NEB #S1419S). Following polyA selection ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA 3′-ends were dephosphorylated in a 10 µL reaction with 2 µL T4 PNK (NEB #M0201S) 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 µL of RecJF (NEB #M0264S). Reactions were incubated for 1 hr at 37°C ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 0.1% BSA (New England Biolabs, cat. no. B9000S), and then resuspended in 200 μl of ATAC-seq lysis buffer ...
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bioRxiv - Genomics 2023Quote: ... Klenow fragment (exo-) (NEB)
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bioRxiv - Molecular Biology 2023Quote: ... using NEB’s KLD enzyme mix (NEB #M0554S) with the appropriate Nme2Cas9 effector plasmid as a template ...
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bioRxiv - Molecular Biology 2023Quote: ... The corresponding regions of a partial mouse Azin1 gene were amplified from mouse tail genomic DNA by using Phusion Hot Start Flex 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Genomics 2023Quote: ... This 17 µl of DNA solution was incubated with 3 µl digestion mixture containing 1 µl BciVI (New England Biolabs, cat. no. R0596S) and 2 µl CutSmart buffer (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... and 2 µl CutSmart buffer (New England Biolabs, cat. no. B7204S, 10×) at 37°C for 2h ...
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bioRxiv - Genomics 2023Quote: ... and 0.5 µl of Sulfolobus DNA polymerase IV (New England Biolabs, cat. no. M0327S) was added to the 18 µl DNA solution and incubated at 55°C for 30 min.
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bioRxiv - Genetics 2023Quote: ... The ICU11_sgRNA1_F/R oligonucleotides were phosphorylated using T4 polynucleotide kinase (New England Biolabs) and hybridized in a thermal cycler (Bio-Rad Laboratories T100) ...
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bioRxiv - Genomics 2023Quote: ... we used the NEB Next PPFE repair kit with Ultra II end prep reaction (New England Biolabs, Ipswich, MA, USA) under recommended conditions and Nanopore ligation sequencing kit SQK-LSK110 ...
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bioRxiv - Genomics 2023Quote: ... Transcriptome libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, UK) according to the manufacturer’s instructions and sequenced with 150-bp paired-end sequencing using the Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were digested with 375U of Mbol (NEB) overnight at 37°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of overhangs was performed with 20,000U of T4 DNA ligase (NEB) for 4h at room temperature with rotation ...
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bioRxiv - Molecular Biology 2023Quote: The cells were infected with ZIKV and DENV2 and lysed using the Luna Cell Ready Lysis reagent (New England Biolabs, UK). Quantification of viral RNA was performed from cell lysates using the virus specific primers and their levels were normalized to mRNA of GAPDH (Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... For these analyses Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX opus real-time 384 system (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and adaptor ligation was performed on beads with the NEBnext Ultra II kit (New England Biolabs E7645). An optimal PCR cycle for final library amplification using NEBnext Ultra II Q5 was determined ...
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bioRxiv - Molecular Biology 2023Quote: RNA 3′-ends were dephosphorylated in a 10 µL reaction with 2 µL T4 PNK (NEB; #M0201S) 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) ...
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bioRxiv - Molecular Biology 2023Quote: ... The dsDNA library was cloned into pBlueScript II KS+ vector by Gibson Assembly (NEB) and electroporated into ElectroMAX DH5α (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... Half the cDNA was subjected to 2nd strand synthesis using Phusion high-fidelity DNA polymerase (NEB) with primers to the 5’ RNA linker and reverse adaptor (5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ and 5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was cleared of RNA by treating with RNase H (NEB). Half the cDNA was subjected to 2nd strand synthesis using Phusion high-fidelity DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and quantitative PCR were performed in one step using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). The primers used are listed in supplemental table (Table ...
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bioRxiv - Molecular Biology 2023Quote: Coupled transcription-translation assays were carried out with the PURExpress kit (New England BioLabs) as described in (Lukasiewicz and Contreras ...
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bioRxiv - Molecular Biology 2023Quote: ... and BL21 (DE3) (NEB) E ...
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bioRxiv - Molecular Biology 2023Quote: ... DH5α (NEB) and BL21 (DE3 ...
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bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of RNA was treated with DNase (DNAse I RNas-free, NEB) for 10-15 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... for 75 min with restriction enzymes PstI and NlaIII (New England Biolabs, UK), followed by heat-inactivation at 65 °C ...
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bioRxiv - Genomics 2023Quote: ... the library vector was pre-digested with BsmBI (New England Biolabs). The ligation product was isopropanol precipitated and electroporated into Stbl4 electrocompetent cells (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... in vitro transcription (NEB E2040S) at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111 ...
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bioRxiv - Genomics 2023Quote: ... coli poly(A) polymerase (NEB, Ipswich, MA, USA). Total RNA was extracted from SINV-infected JW18 cells using TRIzol reagent (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... FFPE samples were repaired using PreCR Repair Mix (NEB), per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... followed by RQ1 RNase-free DNase (NEB, Ipswich, MA, USA) treatment using manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... 2 (TOYOBO) and BsaI-HF (NEB) and incubated at 37℃ for 5 min and 16℃ for 5 min for three cycles ...
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bioRxiv - Microbiology 2023Quote: RT-LAMP reactions were done using the WarmStart Colorimetric LAMP 2X Master Mix kit (M1800, NEB, Hitchin, UK) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Genomics 2023Quote: ... sgRNA was synthesized using NEB’s HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). For generation of transgenic fish ...
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bioRxiv - Genomics 2023Quote: ... and ligated with T4 ligase (NEB, M0202) overnight at 16°C ...
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bioRxiv - Genomics 2023Quote: ... after BsmBI (NEB, R0580L) digestion ...
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bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... ligated to the 5′ adapter with T4 RNA ligase (NEB M0204L), and reverse transcribed with Superscript III (Thermo Fisher 18080-051) ...
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bioRxiv - Molecular Biology 2023Quote: ... A range of cycle numbers was used to amplify the small RNA amplicons using Q5 High-Fidelity DNA polymerase (NEB M0491L). Amplified cDNA samples were loaded on 10% polyacrylamide gel (Bio-Rad 3450051) ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEB Directional Ultra II RNA library prep kit (NEB, E7760) by following the manufacturer’s recommendations (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 μl QuickCIP enzyme (NEB) at 37°C for 10 minutes ...