Labshake search
Citations for New England Biolabs :
9451 - 9500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... RT-qPCR for SARS-CoV-2 from cell lysates was performed using the TaqMan assay for the CDC N1 gene primers and probes from Integrated DNA Technologies (catalog no. 10006600; Integrated DNA Technologies) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) as previously described (6) ...
-
bioRxiv - Immunology 2023Quote: ... digested with BamHI (NEB, R3136S) and KpnI (NEB ...
-
bioRxiv - Immunology 2023Quote: ... or Gibson Assembly Master Mix (New England Biolabs).
-
bioRxiv - Microbiology 2023Quote: ... Klenow fragment (NEB) and adaptor ligated with annealed adaptors (Nimblegen Systems) ...
-
bioRxiv - Microbiology 2023Quote: ... 2 mM vanadyl ribonucleoside complex (NEB, S1402S). The cells were then washed 4 times in wash buffer containing 25% formamide (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... containing 1x NEBuffer 2 (NEB, B7002S), 1 mM ATP ...
-
bioRxiv - Microbiology 2023Quote: ... Double-stranded DNA was synthesized with DNA Polymerase I (New England BioLabs, M0209S) and subsequently fragmented by dsDNA Fragmentase (New England BioLabs ...
-
bioRxiv - Microbiology 2023Quote: ... 1 μl of RNase Inhibitor (New England BioLabs, M0314), and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs, M0437)] at 23°C for 5 h ...
-
bioRxiv - Microbiology 2023Quote: ... All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler ...
-
bioRxiv - Microbiology 2023Quote: Total RNA isolation was performed using the Monarch® Total RNA Miniprep Kit (New England Biolabs). Mycelia were cultured in RY broth (20 g/L rice flour and 2 g/L yeast extract ...
-
bioRxiv - Microbiology 2023Quote: ... Cap17 was also incubated with 50 ng/μL of lambda DNA (NEB #N3011S) or control RNA (Ambion MicrobExpress ...
-
bioRxiv - Microbiology 2023Quote: ... were suspended in 1 × DNA/RNA Protection Reagent (New England Biolabs), and RNA Lysis Buffer (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... and RNA Lysis Buffer (New England Biolabs) was added to the supernatant after centrifugation ...
-
bioRxiv - Microbiology 2023Quote: ... 10U RppH (NEB), and 1U RNAseOUT (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: In vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) with the following modifications ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions were conducted in 25 μL reaction volumes with 12.5 μL of 2x master mix (Biolabs, USA), 1 μL of each forward and reverse primer ...
-
bioRxiv - Microbiology 2023Quote: ... NEBNext® Ultra™ II DNALibrary Prep Kit for Illumina® (New England Biolabs, cat. E7645S) was used to prepare the sequencing libraries as per manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 U DNase I (NEB) were added ...
-
bioRxiv - Immunology 2023Quote: ... DNA was amplified using Luna® Universal Probe qPCR Master Mix (NEB England Biolabs).
-
bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... DNA was amplified using Luna® Universal Probe qPCR Master Mix (NEB England Biolabs).
-
bioRxiv - Immunology 2023Quote: ... Complementary DNA (cDNA) was generated for each replicate using the LunaScript RT Supermix (New England Biolabs) based on the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: Sequence libraries were prepared using the NEBNext® Ultra TM RNA Illumina® (NEB, USA) Library Prep Kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... Variants were subsequently cloned into pGW1-6C by Gibson Assembly (NEB). Nonsense mutations were generated by site-directed mutagenesis using primers onk046 and onk047 to change the rpoB start codon TTG to stop codon TAG ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli (C2987, NEB) was used to propagate plasmids and was cultured in LB medium or grown on LB agar ...
-
bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Deletion variants of ATI2 were amplified from ATI2 (AT4G00355.1) CDS by overlap extension PCR using Phusion High Fidelity DNA polymerase (NEB) and subcloned into pCR™4/TOPO™ vectors (Invitrogen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2015 at 1.25µM and NEBNext High Fidelity 2x Mastermix (New England Biolabs, M0541) in a 50µL reaction (5 minutes at 72ºC ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2.5µL of T4 ligase buffer (NEB), 1µL of T4 ligase (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 8 μl of a mix preheated to 37°C and containing 2.5 U of RNase H (New England Biolabs), 2.5× RNase H reaction buffer (1× buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2.5µL 10mM ATP (NEB), 1µL of synthetic RNA precursor (5pmol/µL) ...
-
bioRxiv - Molecular Biology 2023Quote: ... along with commercial yeast Xrn1 (NEB; M0338) as a positive control (Supplementary Figure X) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and Murine RNase inhibitor (New England Biolabs, M0314S), using a Kontes motorized pestle ...
-
bioRxiv - Molecular Biology 2023Quote: ... 500 ng of RNA were subjected to poly(U) tailing using poly(U) Polymerase (New England Biolabs, M0337S) in 1X NEBuffer 2 for 30 minutes at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmid pAR107 was generated by digesting pRS415 with the restriction enzymes SmaI and SalI (New England Biolabs), PCR amplifying the Gateway cassette from pQZ213 and assembling both products by gap repair in the yeast strain BY4733 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ligating both products with the T4 DNA Ligase (New England Biolabs). Plasmid YEplac181-Sin3-(HA)3 (2µ AmpR LEU2 SIN3- (HA)3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... zebrafish brains were permeabilized with proteinase K (20 mg/ml) (NEB, P8107S) at room temperature for 30min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were ligated to adaptor oligos for multiplex sequencing (NEB, E7335), PCR amplified ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.25M NaCl) and Proteinase K (New England Biolabs Inc (NEB)) were added and crosslinks were reversed overnight at 65C ...
-
bioRxiv - Molecular Biology 2023Quote: pAC8-FLAG-SHLD3 truncation plasmids were generated using Gibson cloning (New England Biolabs) and a pAC8-FLAG-SHLD3 template (Setiaputra et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.25M NaCl) and Proteinase K (New England Biolabs Inc (NEB)) were added and crosslinks were reversed overnight at 65C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ligase reaction mixture (1 U/μL SplintR ligase (NEB, M0375L), 1X buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Paired-end Illumina sequencing libraries were prepared from CUT&RUN enriched DNA using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs) and sequenced on a NextSeq 500 (Illumina) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
-
bioRxiv - Molecular Biology 2023Quote: ... Amylose beads (NEB) were mixed with lysate supernatant for 1.5 h and washed with buffer A ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 1x Phi-29 reaction buffer (NEB) with rolling circle amplification carried out as previously described6 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1mM each in the presence or absence of Phi-29 polymerase (NEB, 3.75U/32ng DNA) and 1x Phi-29 reaction buffer (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transcription reactions were carried out with SP6 polymerase (NEB) for 1 hour at 37°C ...