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Citations for New England Biolabs :
9851 - 9900 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 0.2 mM S-adenosyl-methionine (SAM, NEB), and 2 µL of Vaccina capping enzyme (homemade ...
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bioRxiv - Biochemistry 2024Quote: ... 1X capping buffer (NEB), 0.5 mM GTP (ThermoFischer) ...
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bioRxiv - Biochemistry 2024Quote: The reactions were stopped by adding 1 µL of Proteinase K (NEB) to the sample and incubation at 37°C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... Cytokine 3’UTR fragments and full length 3’UTRs containing CTTT--> CGTT or AUUUA--> AUGUA point mutants containing BamHI and NotI restriction sites were obtained as minigenes from IDT and inserted into pRS_PGK_GFP according to manufacturer’s protocol (T4; NEB). Plasmid DNA was amplified in DH5alpha super-competent bacteria (NEB ...
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bioRxiv - Immunology 2024Quote: ... Plasmid DNA was amplified in DH5alpha super-competent bacteria (NEB) and isolated with NucleoSpin Plasmid Transfection-grade Mini-Prep isolation kit (Machery-Nagel) ...
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bioRxiv - Immunology 2024Quote: ... This tethered mIL-12 construct was inserted into the MSCV ZsGreen plasmid directly upstream of IRES-ZsGreen via digestion of the vector with XhoI/EcoRI (NEB) followed by Gibson Assembly ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin/streptomycin (100U/mL penicillin and 100 µg/mL streptomycin, Nordic Biolabs), and supplemented with 1mg/mL geneticin (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: ... and 0.4 U/µL terminal transferase in 1X TdT buffer (NEB #M0315S)) for 1 hour at 37°C.
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bioRxiv - Genomics 2024Quote: ... a 16 hour RCA reaction was performed at 30°C using a mixture containing 500 µM dNTP (NEB #N0447S), 50 µM amino-allyl-dUTP (Thermo Scientific #R1091) ...
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bioRxiv - Genomics 2024Quote: ... 0.5% Triton X-100) containing 8 U/mL Proteinase K (NEB #P8107S) for 12-16 hours at room temperature ...
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bioRxiv - Genomics 2024Quote: ... 0.5% Triton X-100) containing 1% of 8 U/mL Proteinase K (NEB #P8107S) for 12-16 hours at room temperature ...
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bioRxiv - Genomics 2024Quote: The 15µL of purified DNA was mixed in a PCR reaction containing 1X NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541L), 1 µM foward primer ...
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bioRxiv - Genomics 2024Quote: ... Injection mixes were prepared with 1.3 µl of SpCas9 (20 µM, New England BioLabs, Ipswich, MA), 1.6 µl of annealed crRNA:tracrRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The mixture was digested with 400 units of MspI (New England Biolabs) in NEB Ultra II buffer (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... the fragments were repaired and A-tailed using 15 units of Klenow 3’-5’ exo-(New England Biolabs). After End-repair and A-tailing ...
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bioRxiv - Cancer Biology 2024Quote: ... in NEB Ultra II buffer (New England Biolabs) in a final volume of 57 µL for 2 h at 37°C ...
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bioRxiv - Genomics 2024Quote: Skin biopsies were cut into small pieces and processed with the Monarch Genomic DNA Purification Kit (NEB, Cat. T3010S) or DNeasy blood and tissue kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... Libraries were made according to the manufacturer’s directions for the NEBNext Enzymatic Methyl-seq kit (New England Biolabs, Cat. No. E7120S) using the S220 Focused-ultrasonicator (Covaris ...
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bioRxiv - Genomics 2024Quote: ... the bead array was put into a 1.5 mL centrifuge tube of 200 µL extension buffer (1x NEBuffer 2, 1mM dNTP, 25 units Klenow exo- (NEB M0212L)) ...
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bioRxiv - Immunology 2024Quote: ... An IL10-tCD19 ORF was gene synthesised (GeneArt) and cloned by Gibson assembly (NEB) into an AAV6 transfer plasmid (70) ...
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bioRxiv - Immunology 2024Quote: ... via the NEBuilder® HiFi DNA Assembly (NEB, #M5520AVIAL), and the corresponding ASFV plasmids were constructed for the following characterization ...
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bioRxiv - Biochemistry 2024Quote: ... and DNase I (New England Biolabs) was added to a final concentration of 0.012 U/μl and the reaction incubated for 5 min at 28 °C ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (NEB) and cultured overnight at 37 °C in LB media supplemented with 50 μg/mL kanamycin ...
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bioRxiv - Cancer Biology 2024Quote: ... The adaptors used for multiplexing were purchased separately (Next Multiplex Methylated Adaptors-New England Biolabs). Libraries were size-selected by dual-step purification with Ampure XP Magnetic Beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... corresponding to both strands of the tRNA His promoter from positions −45 to +76 or DNA mutants were end labeled using [γ-32P] adenosine 5′-triphosphate (ATP) and T4 polynucleotide kinase (New England Biolabs) and purified on a 10% acryl/bisacrylamide ...
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bioRxiv - Cancer Biology 2024Quote: ... truncated KQ (NEB, per manufacturer’s instructions with 15% PEG-8000 final ...
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bioRxiv - Cancer Biology 2024Quote: ... libraries were amplified with NEBNext High-Fidelity PCR Master Mix (NEB, Cat# M0541S) using custom Nextera primers ...
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bioRxiv - Cancer Biology 2024Quote: ... The product was purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030), and eluted in 25 mL of water ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5 ml fractions of the protein pool were incubated with 3-fold molar excess of SNAP-Surface Alexa Fluor 546 or SNAP-Surface Alexa Fluor 647 (New England Biolabs) overnight at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (New England Biolabs) by chemical transformation ...
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bioRxiv - Biochemistry 2024Quote: ... kinase-ligase-DpnI (New England Biolabs) ligation of vector and removal of template DNA ...
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bioRxiv - Biochemistry 2024Quote: ... The amidases were cloned into MCS1 of pCDFDuet-1 using Gibson assembly kit (New England Biolabs) after linearization of the vector with NcoI and HindIII (Fermentas) ...
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bioRxiv - Biochemistry 2024Quote: ... 50 nM of the pRRG260 Cyp1A1 plasmid mutants containing 310 bp and 410 bp were linearized using BamHI-HF (NEB) to create a run-off transcription template ...
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bioRxiv - Biochemistry 2024Quote: All mutations were performed using the Quick Change PCR protocol (Q5 Site-Directed Mutagenesis Kit E0554, NEB), and were based on an AMPH-1 pGEX-2T expression vector ...
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at -20°C ...
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bioRxiv - Biophysics 2024Quote: ... RanGTP-His was expressed in BL21(DE3) cells (New England Biolabs) using 0.5 mM IPTG for 12 hr at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR product was extracted using the Monarch DNA gel extraction kit (NEB). The extracted PCR product was Sanger sequenced by Microsynth to confirm its identity.
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bioRxiv - Biochemistry 2024Quote: RT-PCRs were performed on 1:100 diluted RT samples using hot start Taq DNA polymerase (NEB) supplemented with extreme thermostable ssDNA-binding protein (ET SSB ...
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bioRxiv - Biochemistry 2024Quote: ... The molecular weight standard used was the Quick-Load Purple 100 bp DNA Ladder (NEB). The PCR product band corresponding to the expected molecular weight of the RT-PCR product of the intron reverse spliced into the plasmid was excised from the gel ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with extreme thermostable ssDNA-binding protein (ET SSB, NEB), under the manufacturer’s recommended reaction conditions (1X standard Taq reaction buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Blunt-ended PCR products were generated with the Q5 High-Fidelity 2X Master Mix (NEB), followed by 5’-phosphorylation ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified using a Monarch PCR & DNA cleanup kit (NEB), and products were electrophoresed on an agarose TAE gel to confirm the product was of the correct size ...
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bioRxiv - Biochemistry 2024Quote: ... Reverse transcription was carried out using the Bst 3.0 DNA polymerase (NEB) because of its superior strand-displacement activity compared to other commercially available RTs (31) ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then purified with the Monarch RNA cleanup kit (NEB), and the RNA concentration was determined by absorbance at 260 nm on a Nanodrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl of each sample was mixed with 1 µl of 6X Purple Gel Loading Dye (NEB) and electrophoresed at 110 V for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... PCR of the RT product was done with the Q5 High-Fidelity 2X Master Mix (NEB), and two different primer pairs for the 5’- and 3’-splice-sites ...
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bioRxiv - Biochemistry 2024Quote: ... and DpnI digest with a KLD enzyme mix (NEB). The resulting 1894 bp recombinant plasmids were then transformed into chemically competent Top10 E ...
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bioRxiv - Biochemistry 2024Quote: ... PCRs were performed in 100 µL reactions using Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs - NEB), 0.5 µM of each primer ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were linearized by restriction digestion with BspHI (NEB), for which the sole restriction site lies roughly equidistantly (949 bp downstream ...
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bioRxiv - Biochemistry 2024Quote: ... TdT (20 U) and α-32P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h ...