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Citations for New England Biolabs :
9501 - 9550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... RA3 adaptors were ligated to RNA with the T4 RNA ligase kit (NEB, M0242L) for 2 h at 25 °C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
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bioRxiv - Neuroscience 2024Quote: ... Library preparation was performed with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L). SPRI beads (Bulldog Bio ...
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bioRxiv - Neuroscience 2024Quote: ... and end repair was performed by incubating chromatin-bound beads for 1 h at room temperature in 1× T4 ligase buffer with ATP (NEB, B0202), 0.25 mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... DSB ends were blunted with the Quick Blunting kit (NEB, E1201L) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... NEBNext Ultra II master mix (NEB, M0544L), SYBR Green I (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was fragmented using dsDNA fragmentase (NEB, M0348) for 40–50 min at 37 °C to generate 300–800-bp fragments ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated with proteinase K (NEB, P8107S) at 55 °C for 18–24 h ...
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bioRxiv - Neuroscience 2024Quote: ... 1×T4 ligase buffer with ATP (NEB, B0202), 5% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5 µM of sample-specific P5/P7 barcoded primer mix (NEB, E7335S, E7730S, and E7600S) were used to add barcodes and amplify the ATAC-seq samples by quantitative and limited PCR ...
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bioRxiv - Genomics 2024Quote: ... First we re-amplified cDNA by combining 20 ng of the remaining cDNA from channel 2 of each sample together with 50 µl of NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), 2 µl of 12 µM PCR Primer1 (5’-CTACACGACGCTCTTCCGATCT-3’) ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011 ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs) following the manufacturer’s protocol (including RNase H and DNase I digestions) ...
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bioRxiv - Neuroscience 2024Quote: AAV transfer plasmids were generated through modifications of existing plasmids using HIFI DNA assembly cloning (NEB). pAAV-Ef1a-Con/Fon N2cG ...
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bioRxiv - Neuroscience 2024Quote: ... into a pAM-FLEX AAV2 backbone that had been modified to replace DIO with fDIO sequences.27 PCR amplicons were generated using Q5 DNA polymerase (NEB). Plasmids were propagated at 30°C in Stable cells (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were propagated at 30°C in Stable cells (NEB). To produce recombinant AAVs ...
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bioRxiv - Neuroscience 2024Quote: ... and the Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs). cDNAs for each of the candidate interacting proteins were synthesised and cloned into the pCDNA3.1(+ ...
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bioRxiv - Physiology 2024Quote: ... 1 μl 10 mg/ml Proteinase K (Biolabs, #P8107S), 1 μl 10 mg/ml RNase A plus 4.5 μl 5 M NaCl ...
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bioRxiv - Physiology 2024Quote: ... The lysate was amplified using OneTaq MasterMix (New England Biolabs) according to manufacturer instructions with primers suggested by Jackson Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... site-directed mutagenesis was performed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA), adhering to the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently cloned into the pGL3-promoter vector downstream of the firefly luciferase reporter gene by Gibson Assembly (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... Reverse: 5’-GCTGCCAGTCCAATAGAGGG-3’) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) with SYBR Green detection on the CFX96 (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... flanking the Cas9 cut site were amplified from ORL-strain genomic DNA via PCR and cloned into a T2A-QF2-3XP3-dsRed plasmid backbone (linearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S) ...
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bioRxiv - Neuroscience 2024Quote: ... We then transcribed sgRNA in vitro using the HiScribe T7 Kit (NEB, E2040S) with an 8-9 hr incubation at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... and generated dsDNA template for transcription of the sgRNA via template-free PCR with partially overlapping PAGE-purified primers using the NEBNext High-Fidelity polymerase (NEB, M0541S). We then transcribed sgRNA in vitro using the HiScribe T7 Kit (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 1 x 107 GCps were incubated overnight with 1500U of HindIII (NEB, R0104T) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated at 55°C for 10 min prior to addition of 2U RNAseH (New England Biolabs, #M0297S) and further incubation at 55°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then incubated in a ligation mix (2.5 U/μL SplintR ligase (NEB), 20 μg/mL BSA ...
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bioRxiv - Physiology 2024Quote: ... a separate set of lysates was treated with Peptide-N-Glycosidase F (PNGase F; New England BioLabs, #P0704) according to the manufacturer’s recommendations and blotted as shown in Table 1 ...
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bioRxiv - Zoology 2024Quote: ... an Illumina library with an average insert size of 350 bp was constructed using NEB Next Ultra DNA Library Prep Kit (NEB, Beijing, China) with PE 150 bp mode.
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bioRxiv - Physiology 2024Quote: ... A NEB Next® Ultra Library Preparation Kit (NEB E7530S) was used to prepare 0.5 µg of total RNA for RNA-seq ...
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bioRxiv - Physiology 2024Quote: ... mRNA was purified by NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) from Turbo-DNAse treated total RNA ...
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bioRxiv - Neuroscience 2024Quote: ... or NEBuilder® HiFi DNA Assembly Master Mix (NEB); 1.9 kb sequence of mouse MBP promoter (a gift from Yasuyuki Osanai) ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual RNA was digested with 2 units RNaseH (NEB) for 20 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with DNase I (NEB) and subjected to qRT-PCR.
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bioRxiv - Molecular Biology 2024Quote: ... We employed the Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, American), in a T100 thermocycler (Bio-RAD Inc) ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids were assembled using Gibson assembly (NEB HiFi DNA Assembly Master Mix E2621) or restriction-ligation (NEB Quick Ligase M2200) ...
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bioRxiv - Molecular Biology 2024Quote: ... or restriction-ligation (NEB Quick Ligase M2200). The list of plasmids generated in this study can be found in supplementary table and their annotated sequence maps as supplementary information ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Samples were depleted of ribosomal RNA (rRNA) using the NEBNext rRNA Depletion kit v2 (NEB, E7400L). Libraries for sequencing were prepared using the NEBNext Ultra II directional library kit (NEB ...
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bioRxiv - Biophysics 2024Quote: ... We digested the pBS-parS for 2 h at 37°C using NotI-HF or XhoI restriction enzymes (New England Biolabs) and heat-inactivated for 20 min at 80°C ...
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bioRxiv - Biophysics 2024Quote: ... We combined the plasmid backbone with the colony PCR insert by mixing them in molar ratio 1:3 in the 2xHiFi mix (New England Biolabs) to obtain the final plasmid of 4175 bp ...
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bioRxiv - Biophysics 2024Quote: ... BbvCI nicking enzyme (New England Biolabs) capitalizing on a pre-existing recognition site in the metS gene ...
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bioRxiv - Biophysics 2024Quote: ... coli NEB5alpha cells (New England Biolabs), and verified the presence of insert in grown colonies the following day by sequencing using MT030 and MT031 (Table 2) ...
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bioRxiv - Biophysics 2024Quote: To construct the circular DNA for AFM experiments we used a commercially available pGGA plasmid backbone (New England Biolabs). We linearized the pGGA plasmid using MT032 and MT033 primers (Table 2) ...
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bioRxiv - Neuroscience 2024Quote: ... Between 72-92 ng of RNA per sample was subjected to rRNA depletion via the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat; NEB #E7400) using the manufacturer’s protocol except that a Monarch® RNA Cleanup Kit (10 µg ...
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bioRxiv - Neuroscience 2024Quote: ... using the manufacturer’s protocol for use with rRNA-depleted RNA and NEBNext® Multiplex Oligos for Illumina® (NEB# E6448). Samples were pooled and sequenced 2×150 nt paired-end on an Illumina® Novaseq 6000 instrument.
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bioRxiv - Neuroscience 2024Quote: ... The rRNA-depleted RNA was converted [15] into Illumina-compatible DNA libraries via the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads kit (NEB# E7765) using the manufacturer’s protocol for use with rRNA-depleted RNA and NEBNext® Multiplex Oligos for Illumina® (NEB# E6448) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA fragments were converted to library using the NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs, #E7330S) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... coli poly(A) polymerase (New England Biolabs, #M0276S) prior to 3’ RACE.