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Citations for New England Biolabs :
901 - 950 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the libraries were size selected using NEBNext Sample Purification Beads (NEB cat. # E7767) following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Beads were re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was mixed and incubated with chitin beads (New England Biolabs). The PSM polypeptides were eluted with 50 mM DTT to cleave the intein ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA was digested on-beads using 1U of DNAse I (NEB M0303) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was submitted to the center where poly A RNA was purified using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA). Next generation sequencing (NGS ...
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bioRxiv - Genetics 2021Quote: ... We isolated poly(A)+ RNA from 1 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... for A673 experiments was used to prepare barcoded sequencing libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, cat. no. E7490) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-Seq libraries were generated from 2 µg RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7490 and #E7420). Libraries were quantified using the Agilent DNA 7500 Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples had an RQN > 7.5 and strand-specific sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® in conjunction with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs).
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bioRxiv - Genetics 2022Quote: ... mRNA was purified and enriched from 1 µg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, catalog no. E7490L). RNA fragmentation ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation modules and Ultra II Directional Library Prep kits (New England BioLabs, Inc, MA, USA) according to standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Poly-A RNA was isolated from the total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs; E7490L or E7490S) and then quantified by a Qubit 3.0 Fluorometer (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... and mRNA was isolated from ∼1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Cat. No. / ID: E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA was isolated from ∼1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Cat. No. / ID: E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” combined with “NEB Ultra II polyA mRNA magnetic isolation” for mRNA enrichment (New England Biolabs, Ipswich, MA, USA). Libraries were pooled and sequenced (single-end ...
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bioRxiv - Immunology 2023Quote: ... 150 ng of total RNA were purified using the NEBNext poly(A) mRNA Magnetic Isolation module (New Englands Biolabs Inc., Ipswich, MA, USA) and used as a template for cDNA synthesis by reverse transcriptase with random primers ...
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bioRxiv - Genomics 2023Quote: ... We purified and enriched mRNA from 1 ug of total RNA using the NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs, catalog no. E7490L). RNA fragmentation ...
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bioRxiv - Molecular Biology 2023Quote: 1 µg of total RNA was used to perform mRNA isolation using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB Cat. No. E7490). The resulting mRNA material was used to prepare the libraries with the use of NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 μg RNA was used to isolate poly(A)-enriched RNA with NEBNext® Poly(A) mRNA Magnetic Isolation Module (NE Biolabs, Ipswich, MA) that was used to prepare RNA sequencing libraries with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NE Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... of 8 or greater were subjected to Poly-A enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB catalog number E7490). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog number E7760L) ...
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bioRxiv - Developmental Biology 2024Quote: ... of 8 or greater were subjected to Poly-A enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB catalog number E7490). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog number E7760L ...
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bioRxiv - Cancer Biology 2023Quote: Polyadenylated RNA enrichment and library preparation were conducted using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7490S and #E7760S), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA sequencing libraries were enriched for mRNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (S7590S; New England Biolabs, Ipswitch, MA) and prepared using NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (E7765 ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of total RNA was diluted with nuclease-free water to a final volume of 200 µL and the poly(A) RNAs were isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module protocol (#E7490, New England BioLabs Inc, Massachusetts, USA). Nuclear and Cytoplasmic poly(A ...
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bioRxiv - Molecular Biology 2023Quote: 500 ng of total RNA was used to perform mRNA isolation using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB Cat. No. E7490). Isolated mRNA material was used to prepare the libraries using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... The bead slurry was directly treated with 3 µL Klenow (3’→5’ exo-) (NEB) in 50 µL NEB Buffer #2 with 0.2 mM ATP at 37 °C for 30 min to add 3’ overhangs to DNA ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was incubated with 37.5 mg of oligo(dT)25 beads (NEB S1419S) for 30 min at 4°C on a Nutator ...
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bioRxiv - Biochemistry 2019Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were washed with 1x and 5x PMPG and treated with alkaline phosphatase (NEB) to de-phosphorylate DDX43-crosslinked RNA on beads ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration and clean up steps were performed using AMPureXP beads (New England Biolabs). 12 samples (from 12 isolates ...
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bioRxiv - Cell Biology 2019Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Genomics 2021Quote: ... Bead pellets were then resuspended with 45μl elution buffer supplemented with proteinase K (NEB) and incubated for 1 hour at 55°C and then 8-10 hours at 65°C to revert formaldehyde cross-linking ...
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bioRxiv - Molecular Biology 2021Quote: ... Mini pools were size-selected and purified using AMPure XP Beads (BioLabs Inc., USA) prior to quantification on Qubit (Qubit™ dsDNA HS Assay Kit ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 μM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction was applied to 10 ml of chitin beads (New England Biolabs) equilibrated with chilled buffer A ...
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bioRxiv - Molecular Biology 2021Quote: The DNA-complex-coated beads were incubated with the end-repair enzyme mixture (NEB) at 20 °C for 30 min to repair the DNA ends and then with Klenow exo- (NEB ...
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bioRxiv - Genomics 2021Quote: ... Amplicons were immediately cleaned up with AMPure XP beads (New England Biolabs, ref. A63880) and libraries were prepared using the ligation sequencing kit (SQK-LSK109 ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were washed and incubated with 50U shrimp alkaline phosphatase (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... On-Bead 5’ Decapping reaction was done using NEBuffer 2 buffer (NEB, B7002S ®). Off-Bead Reverse Transcription followed the instructions in SuperScript ® IV Reverse transcriptase kit ...
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bioRxiv - Microbiology 2023Quote: ... and followed by second strand synthesis on bead using klenow (New England Biolabs, NEB) as described [40] ...
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bioRxiv - Microbiology 2023Quote: ... and followed by second strand synthesis on bead using klenow (New England Biolabs, NEB) as described [40] ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP-EXO1-bound beads were first dephosphorylated by lambda phosphatase (New England BioLabs, P0753S) to remove all existing phosphorylation ...
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bioRxiv - Molecular Biology 2023Quote: ... deaminase treated RNA was purified with NEBNext sample purification beads (E7104S, NEB, Ipswich, MA) using 1.2x beads to sample volume ratio ...
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bioRxiv - Plant Biology 2022Quote: ... Paramagnetic beads coupled with oligo d(T)25 were combined with total RNA to isolate poly(A)+ transcripts based on NEBNext® Poly(A) mRNA Magnetic Isolation Module manual (New England BioLabs Inc., Massachusetts, USA). All libraries were constructed according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... The library was prepared by a Ribo-Zero protocol (250-300 bp insert strand specific library with rRNA removal using NEB Ribo- Zero Magnetic Kit). Paired-end sequencing produced 150 bp reads ...
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bioRxiv - Cell Biology 2021Quote: ... Twelve total RNA samples were subjected to mRNA enrichment using mRNA NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, cat. no. E7490L), from which libraries were derived ...