Labshake search
Citations for New England Biolabs :
1001 - 1050 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Genomics 2021Quote: ... Beads were resuspended in 50 μL PCR master mix (25 μL 2X Phusion HF buffer (NEB), 1 μL 12.5 μM Nextera Ad1 primer (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were first treated with T4 PNK (NEB, M0201L; 20 U in 80 μl reaction volume) in the absence of ATP (37°C ...
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bioRxiv - Genomics 2021Quote: ... The PCR reactions were cleaned up using 0.9X NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 25μL of 0.1X TE ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µL of the solution was mixed with 15 µL of amylose beads (New England Biolabs). Samples were placed into PierceTM micro-spin columns (Thermofischer ...
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bioRxiv - Genomics 2022Quote: ... a second bead binding was performed followed by a 5’ de-capping using RppH (NEB M0356S). The 5’ end of the transcript was phosphorylated using PNK (NEB M0201L ...
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bioRxiv - Genomics 2022Quote: ... Beads were washed and separated on a magnet before dA-tailing with Klenow exo minus (NEB). A final wash was performed before Illumina adaptor ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21 (DE3) strain and purified with amylose agarose beads (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were eluted from beads with 11 μl 1x TE and 1.5 μl USER enzyme (NEB) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: Libraries were prepared on beads using the NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645). First ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated in 100 μl end-repair mix containing 1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Cell Biology 2022Quote: ... The beads to which GFP-DSB-1 had been immobilized were subject to the phosphatase assay (NEB lambda phosphatase #P0753S ...
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bioRxiv - Genomics 2020Quote: ... the beads were equilibrated once in 100 μL 1x NEB Quick Ligation Reaction Buffer (New England Biolabs). Then the beads were resuspended again in 50 μL 1x NEB Quick Ligation Reaction Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Microbiology 2021Quote: ... total RNAs were extracted from Caco-2 cells and followed by mRNA purification (Oligo dT beads, NEB). The mRNAs were fragmented and converted into double-stranded DNA by reverse transcription and then second-strand synthesis (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated ...
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bioRxiv - Microbiology 2021Quote: ... the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated ...
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bioRxiv - Genomics 2022Quote: Beads were resuspended in 80 µl T4 PNK reaction mix (3 µl T4 PNK (NEB cat# M0201B), 97.2 mM Tris pH 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were resuspended in 25 μl cDNA amplification mix (1X Phusion HF PCR Master Mix (NEB, #M0531L) and 0.5uM P5 and P7 short primer mix (see Supplementary Table 2) ...
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bioRxiv - Microbiology 2022Quote: ... Subsequent on-bead PCR indexing-amplification of tagmented DNA was performed using 2x Phusion Master Mix (NEB) and custom-ordered indexing primers (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
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bioRxiv - Systems Biology 2019Quote: ... the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5′-phosphorylated ...
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bioRxiv - Biochemistry 2019Quote: ... Washed beads were incubated with 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) in a head-over-head rotator overnight at 37 °C.
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-bead 3’RNA adapter ligation using high concentration T4 RNA Ligase I (NEB, #M0204L). IP efficiency was verified by immunoblot of 20% of the IP samples ...
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bioRxiv - Immunology 2020Quote: ... mRNA was isolated from purified 1 ng total RNA using oligo-dT beads (New England Biolabs, Inc). NEBNext Ultra™ RNA kit was used for full-length cDNA synthesis and library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... was ligated to the RNA fragments on bead using T4 RNA ligase 2 truncated KQ (NEB M0373) at 16°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were drained of all liquids before 2 mg/ml of Proteinase K (New England Biolabs) diluted in PK buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... beads were resuspended in 200 μl CIP mix (20 units of calf intestinal alkaline phosphatase (M0290, NEB) in CIP buffer ...
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bioRxiv - Genomics 2021Quote: ... and washed several times before being resuspended the beads in 20 µl NEBuffer 2 (New England Biolabs). The final library was generated using the Illumina TruSeq kit (Illumina ...
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bioRxiv - Genomics 2021Quote: ... cDNA was pooled and cleaned using SPRI beads and excess primers digested by incubation with ExonucleaseI (NEB; 30 min @ 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Poly(A) RNA was isolated with two rounds of selection using oligo-(dT)25 beads (NEB, S1419S). 50 ng of poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... Complexes were captured from 25 μl volumes by incubation with 2 μg of hydrophilic streptavidin beads (NEB) and incubated for 30 minutes with frequent agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... and adaptor ligation was performed on beads with the NEBnext Ultra II kit (New England Biolabs E7645). An optimal PCR cycle for final library amplification using NEBnext Ultra II Q5 was determined ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic libraries were prepared with NEBNext Ultra II FS DNA Library Prep with Beads (New England Biolabs), with single indexes ...
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bioRxiv - Neuroscience 2023Quote: ... beads were washed twice with coIP-solution and finally dissolved in RNA protection buffer (New England Biolabs).
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The product was purified with 1.8× Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by addition of 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the bound TfR was digested on beads with EndoH according to the manufacturer’s recommendations (New England Biolabs). Eluates were analysed by lectin or Western blotting as described above.
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bioRxiv - Cell Biology 2020Quote: ... Dissolved peptides were incubated with antiKcr antibody-coated agarose beads (Catalog No. PTM-503, PTM Biolabs, Hangzhou, China) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed beads were resuspended in 100ul DNaseI digestion mix (1X DNase1 digestion buffer with 5ul DNase1 (NEB)) and incubated at 37°C for 20min ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was then loaded and incubated in a gravity column containing 25 mL of chitin bead slurry (NEB), then washed with 400 mL column buffer (20 mM TrisHCl pH 8 ...
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bioRxiv - Genomics 2020Quote: ... Then the beads were equilibrated once in 100 uL 1x NEB T4 DNA ligase buffer (New England Biolabs) followed by removal of the supernatant ...
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bioRxiv - Molecular Biology 2019Quote: ... centrifuged 10min at 4000g and incubated with oligo(dT)25 beads 30min at 4°C (New England Biolabs). Remaining steps for polyA RNA isolation were performed as described [18] ...