Labshake search
Citations for New England Biolabs :
1051 - 1100 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
-
bioRxiv - Genomics 2021Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
-
bioRxiv - Systems Biology 2020Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
-
bioRxiv - Genomics 2021Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
-
bioRxiv - Microbiology 2020Quote: ... the sheared DNA of the chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs), followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated ...
-
bioRxiv - Genomics 2021Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
-
bioRxiv - Cancer Biology 2022Quote: ... The DNA library was prepared using NEBNext Utra II DNA Library Prep with Sample Purification Beads (NEB #E7103S) according to the manufacturer’s instruction followed by Sequencing on the Illumina HiSeq conducted by GENEWIZ ...
-
bioRxiv - Molecular Biology 2019Quote: ... samples were then radiolabeled on bead with 50ul of the following mix: 5 ul 10X PNK buffer (NEB), 1 ul RNaseOUT ...
-
bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs] ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in Fast AP Master Mix (1x Fast AP Buffer containing 80U RNase Inhibitor (M0314, NEB), 2U TURBO DNase (AM2238 ...
-
bioRxiv - Immunology 2021Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
-
bioRxiv - Genomics 2021Quote: ... Size selection for fragments of approximately 450-550 bp was performed using AMPure® XP beads (NEB, UK). Briefly ...
-
bioRxiv - Genomics 2023Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S) according to the manufacturer’s directions with NEBNext Multiplex Oligos for Illumina (NEB E6440) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
-
bioRxiv - Biochemistry 2023Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR or SNAP-AlexaFluor-647 (New England Biolabs) for 10 min at RT ...
-
bioRxiv - Immunology 2023Quote: ... mRNA was isolated from 50 ng of purified total RNA using oligo-dT beads (New England Biolabs, Inc). NEBNext Ultra(tm ...
-
bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ul-tra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Library preparation was performed on beads using NEBNext ChIP-Sequencing Library Prep Master Mix Set for Illumina (NEB). The manufacturer’s instructions were followed ...
-
bioRxiv - Genomics 2023Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB #E7645). End repair was carried out by resuspending beads in 50 μL 1 × EB buffer ...
-
bioRxiv - Genomics 2023Quote: ... the oxidized DNA was purified with 1.8X Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by adding 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB) for 3 h at 37⁰C ...
-
bioRxiv - Plant Biology 2024Quote: ... The beads were then boiled in SDS-loading buffer for subsequent western blotting using MBP antibodies (NEB, E8032S). Histone peptides utilized in this study were synthesized by EpiCypher and included H3 (1–32 aa) ...
-
bioRxiv - Genomics 2020Quote: ... End-repair and A-tailing were performed on beads using NEBnext library preparation endrepair and A-tailing modules (NEB). Illumina adaptors were ligated and libraries were amplified by 8 cycles of PCR ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated for 30 min at 37°C in 100 μl A-tailing mix (1x NEB buffer ...
-
Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: Biotinylated RNA was resuspended in binding buffer (0.5M NaCl, 20mM Tris-HCl pH 7.5, 1mM EDTA) and isolated using Hydrophilic Streptavidin Beads (NEB S1421S). Isolated RNA was reverse transcribed using the barcoded primer OWG921 and Superscript III (Invitrogen 18080093) ...
-
bioRxiv - Genomics 2020Quote: ... Libraries were amplified on the streptavidin beads using the EpiMark Hot Start Taq (New England Biolabs, Cat. N: M0490) using following program ...
-
bioRxiv - Molecular Biology 2019Quote: ... Beads were washed twice with ELBS and suspended in 20 μL PMP buffer supplemented with 1 mM MnCl2 (NEB). 200 units λ protein phosphatase (NEB ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We prepared libraries using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (New England BioLabs). Final fragment sizes and concentrations were confirmed using a TapeStation 2200 system (Agilent) ...
-
bioRxiv - Cell Biology 2021Quote: ... beads were pelleted and resuspended in 100 μl of phosphatase buffer with lambda protein phosphatase (P0753; New England Biolabs) before agitation at 30°C for 30 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... The adaptor-ligated DNA was hybridized to custom Agilent biotinylated oligonucleotide probes across a 700bp region (53032 probes; 4.684 Mbp oligo) and then pulled-down by Dynabeads M-270 Streptavidin beads (NEB). The post-capture DNA library was amplified with STARR_in-fusion_F and STARR_in-fusion_R primers (Supplementary Table 1) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
-
bioRxiv - Molecular Biology 2020Quote: ... Beads were then washed once in 5 volumes of binding buffer (100μL, 1xPBS containing 1mg/mL of BSA (NEB)) for 10 minutes at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Annealed adapters were ligated onto DNA by resuspending the beads in a 100 μL reaction containing Quick Ligase (NEB) and 0.1 μM annealed i5/i7 adapter ...
-
bioRxiv - Neuroscience 2022Quote: ... so the bead-bound proteins were dephosphorylated in wash buffer containing 1:50 Lambda Protein Phosphatase (New England Biolabs) and 1 mM MnCl2 to make the phosphosites more accessible for a kinase assay ...
-
bioRxiv - Plant Biology 2022Quote: ... The NEBNext Ultra II RNA Library Prep with Sample Purification Beads kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for library preparation and libraries were sequenced in two lanes on the 50 bp single-read Illumina HiSeq 4000 platform at the California Institute for Quantitative Biosciences (QB3 ...
-
bioRxiv - Genetics 2020Quote: ... the beads were resuspended in 75 μL of ChIP Dilution Buffer and 5 μL of BSA (New England BioLabs) and incubated at 4°C overnight with rotation for blocking ...
-
bioRxiv - Systems Biology 2019Quote: ... the beads were incubated 16h at 16°C in 1xPNK buffer with 40U T4 RNA ligase I (NEB, M0204L), and 1 μl 100 μM L5 adapter (Supplementary Table 5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was annealed and the strand extending from the bead-bound adapter was added using a Bst 2.0 DNA polymerase (NEB). The reaction was then stopped ...
-
bioRxiv - Microbiology 2021Quote: ... Library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765S). Sequencing was carried out at the Max Planck Genome Centre (Cologne ...
-
bioRxiv - Genomics 2020Quote: ... the size selection of Adaptor-ligated DNA was carried out using NEBNext Sample Purification Beads (New England Biolabs, USA) followed by PCR enrichment of Adaptor-ligated DNA following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... The RNA libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer's recommendations and Oligo (dT) beads (NEB) were used to isolate poly (A ...
-
bioRxiv - Biochemistry 2021Quote: ... MBP-ShTnsB and the truncated derivatives ShTnsB G190-F584 and ShTnsB G190-L494 (62.5 μmol) were immobilized on 25 μl amylose agarose beads (New England Biolabs) in pull-down buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Genomics 2020Quote: ... library DNA was recovered using AMPure XP beads (1.8X concentration) and PCR amplified for 15 cycles (all enzymes from New England Biolabs). Libraries were sequenced on an Illumina NextSeq in 37×37 paired end mode ...
-
bioRxiv - Molecular Biology 2021Quote: ... The beads were then resuspended in Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs), and the bait-associated complexes were eluted for 1-1.5 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... the beads were incubated with 1 µl CK2 enzyme and 1X CK2 reaction buffer (NEB Inc; catalog no. P6010S) supplemented with 200 µM ATP and 30 mM MgCl2 and rotated for 1 h at 30ºC ...
-
bioRxiv - Biochemistry 2022Quote: ... The beads were covered with 30 μL of reverse transcription mixture containing dNTPs (0.5 mM each, New England BioLabs), acetylated BSA (1,5 μg) ...
-
bioRxiv - Biochemistry 2022Quote: ... and the poly(A) RNA was isolated with two rounds of selection using oligo-(dT)25 beads (NEB, S1419S). The purified poly(A ...