Labshake search
Citations for New England Biolabs :
801 - 850 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Poly-adenylated RNA was enriched from 1ug of RNA for each sample with the NEBNext® PolyA mRNA Magnetic Isolation Module (NEB), incubated at 94°C for 15 min and double-strand cDNA was synthesized using SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Poly (A) mRNA was isolated using NEBNext Poly(A) mRNA Magnetic isolation module (New England Biolabs, Ipswich, MA, USA. NEB#E7490). After first strand and second strand and second strand cDNA synthesis ...
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bioRxiv - Genomics 2021Quote: ... Poly (A) mRNA was isolated using NEBNext Poly(A) mRNA Magnetic isolation module (New England Biolabs, Ipswich, MA, USA. NEB#E7490). After first strand and second strand and second strand cDNA synthesis ...
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bioRxiv - Immunology 2020Quote: ... RNA sequencing libraries were prepared from 500 ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit with Poly-A mRNA magnetic isolation (NEB #E7490) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 and 2) and NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Library quantification was performed by real-time PCR using the KAPA Library Quantification Kit - Illumina Platforms - Complete kit (Universal ...
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bioRxiv - Immunology 2022Quote: ... Total RNAs were subsequently processed to generate an mRNA-seq library using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), NEBNext Ultra RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 µg of DNase-free RNA was subject to poly-A purification and library preparation with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490S) in combination with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S) ...
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bioRxiv - Genomics 2022Quote: ... 1ug of total RNA from three samples were individually poly(A) selected using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Poly(A)+ concentration and rRNA depletion were assessed using Qubit and TapeStation ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were made using 0.5 μg RNA per sample with NEBNext Ultra™ RNA Library Prep Kit for Illumina with the Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... A polyadenylated fraction was purified from the total RNA (1 µg) using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). This fraction was used to construct cDNA library using a previously described method [91] ...
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bioRxiv - Molecular Biology 2022Quote: ... and the resulting libraries prepared for Illumina sequencing using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs: E7490) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Pathology 2023Quote: ... 500 ng of total RNA was used as input for the NEBNext® Poly(A) mRNA Magnetic Isolation Module’ (#E7490L, New England Biolabs (NEB)) in accordance with the manufacturer’s standard instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA samples were enriched for mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB: New England Biolabs, Ispwich, MA); and library preparation was carried out by RNA fragmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA samples were enriched for mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB: New England Biolabs, Ispwich, MA); and library preparation was carried out by RNA fragmentation ...
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bioRxiv - Genetics 2023Quote: ... or 300 ng (day 21) of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs E7490L). Libraries were prepared using the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (E7775S ...
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bioRxiv - Biochemistry 2023Quote: ... The total RNA was treated by DNase I (5 U per 100 μg total RNA) and mRNAs were enriched by NEBNext Poly(A) mRNA magnetic isolation module (NEB E7490L). 8-10 μg of mRNA from each sample was fragmented to the size of ~120-nt by Ambion Fragmentation reagent (Thermo Scientific AM8740 ...
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HTLV-1 Splice Sites in Prevalent Gene Vectors Cause Splicing Perturbations in Transgenic Human CellsbioRxiv - Genomics 2023Quote: ... One μg of total RNA was used to select polyadenylated RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, NEB). Half of the mRNA per sample was used for cDNA synthesis using the Superscript IV transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Molecular Biology 2023Quote: ... Poly-A containing RNA was enriched from the total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490S) and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S) ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA was isolated from ∼1 μg of total RNA using NEB Next Poly (A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
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bioRxiv - Neuroscience 2023Quote: ... using the NEBNext Ultra II Directional Library prep kit for Illumina in conjunction with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and unique dual indices (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-Seq libraries were prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext® UltraTMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced using the NovaSeq 6000 system (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Thirty pg of eGFP mRNA was spiked into each fraction and RNA was extracted as described in “RNA extractions.” Five µg of extracted RNA from each fraction was subjected to polyA-enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and sequenced on an Illumina Hi-Seq 2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA Magnetic Isolation Module (E7490L) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of purified RNA for each sample was enriched for polyadenylated mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and then used as input for the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770S) ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Protein-bound RNAs were end-repaired on beads using T4 PNK (NEB) and 3’-end-labeled with azide-dUTP using yeast poly(A ...
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bioRxiv - Genomics 2020Quote: ... mRNA was enriched using NEBNext Poly(A) mRNA isolation beads (NEB #E7490) and libraries were assembled using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then incubated with 100 μl reaction buffer (containing 1X NEB heparinase mix diluted in dH2O ...
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bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
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bioRxiv - Systems Biology 2021Quote: ... Beads were resuspended at 5 µg µl-1 followed by MseI (NEB) digestion according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 50 μL of 1X NEBuffer 2 (NEB, B7002S) containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo- ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was purified using streptavidin beads (New England Biolabs, Ipswich, MA, S1421S) and ethanol precipitated with the co-precipitate GlycoBlue (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... and once with 2 bead volumes of 1X RNase H Buffer (NEB; 50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was extracted by treating the beads with 4U proteinase K (NEB) for 2 hrs at 55 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Purified MBP-SE and MBP-GFP were bound to amylose beads (NEB) by overnight incubation at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... 3’-end RNA dephosphorylation was performed on beads with PNK (NEB M0201L) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Double stranded cDNAs were purified by NEBNext Sample Purification Beads (NEB, E7767), and then the end prep reaction was performed followed by ligation with sample-specific adaptor and index ...
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bioRxiv - Biochemistry 2021Quote: Amylose pulldowns were performed using pre-blocked Amylose beads (New England BioLabs) in a pulldown buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Beads were then treated with ExoI to digest unoccupied primer sites (NEB).
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bioRxiv - Genetics 2020Quote: ... 50 μL beads and 0.5 mg/mL BSA (NEB B9000S; Ipswich, MA) were added to each fragmented chromatin sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Size selection was performed using NEBNext Sample Purification Beads (New England Biolabs), and indexes were added using NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Plant Biology 2022Quote: ... Messenger RNA selection was performed using NEBNext Oligod(T)25 beads (NEB) incubated at 65 °C for 5 min followed by snap-chilling at 4 °C to denature RNA and facilitate binding of poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected and incubated with SNAP beads (New England BioLabs) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The bead bound α-βγδε fragments were digested with BsaI-HF (NEB) to prepare the 3’ end of the DNA fragments for the subsequent ligation step.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated with pre-equilibrated Amylose beads (Biolabs) for 2 h at 4°C with gentle shaking to bind MBP-TEV-NAP1 ...