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Citations for New England Biolabs :
951 - 1000 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, Cat. No. E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Paramagnetic beads coupled with oligo d(T)25 are combined with total RNA to isolate poly(A)+ transcripts based on NEBNext® Poly(A) mRNA Magnetic Isolation Module manual (New England BioLabs Inc., Massachusetts, USA). Prior to first strand synthesis ...
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bioRxiv - Physiology 2023Quote: ... mRNA isolation and cDNA library preparation were completed with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Inc., Ipswich, MA, USA) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... Polyadenylated RNA was captured from 50 ng of total RNA per sample and libraries were prepared using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Paired-end sequencing was done at the Vanderbilt VANTAGE core laboratory (Vanderbilt University ...
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bioRxiv - Immunology 2022Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... beads were incubated in 200 µL exonuclease mix (10µl Exonuclease in 1xexonuclease buffer, NEB #M0293) for 45 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion protein on glutathione beads was incubated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... PCR reactions were cleaned up using 0.9X NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 25μL of 0.1X TE ...
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bioRxiv - Genomics 2019Quote: ... bead-nuclei were resuspended in 190 µL RNA ligase solution (1× RNA ligase buffer (NEB), 4.5 µM bridge adapter ...
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bioRxiv - Microbiology 2019Quote: ... and the product library was cleaned 3 times with XP AMPure Beads (New England BioLabs) to remove short fragments and primers (bead ratios 1X and 0.6X ...
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bioRxiv - Cell Biology 2019Quote: ... The bead-bound protein was incubated with 3 µM SNAP-Surface Alexa Fluor 647 (NEB) for 40-60 min at 4°C (to fluorescently label the protein) ...
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bioRxiv - Genomics 2021Quote: ... DNA libraries were purified with 1X volume of NEBNext® Sample Purification Beads (NEB #E7103) and eluted with 20 μL nuclease free water ...
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bioRxiv - Bioengineering 2020Quote: ... The obtained bead-bound cDNA product then underwent Exonuclease I treatment (New England Biolabs; M0293M) to remove excess primer before proceeding with second strand synthesis.
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bioRxiv - Genomics 2022Quote: ... We performed a second bead binding followed by a 5’ decapping with RppH (NEB, M0356S). RNA was phosphorylated on the 5’ end using T4 polynucleotide kinase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’ ligation (on-bead) with a barcoded RNA adapter followed using T4 RNA Ligase (NEB). Samples were again stringently washed ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were resuspended in 40 μL of 1X T4 DNA ligase reaction buffer (NEB, B0202S) containing 375 nM of Second ligation adapter (synthesized by IDT ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in a 100 μL blunt enzyme mix reaction (Quick Blunting Kit, NEB) with half the enzyme concentration of the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The DNA library was purified with 1x volume of NEBNext Sample Purification Beads (NEB #E7103) and eluted with 40 μl of 0.1 X TE buffer.
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bioRxiv - Cell Biology 2020Quote: ... the supernatant removed and the beads resusbended in 20 μl 1x CutSmart (New England Biolabs), 1x Maxima H-RT buffer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were mixed with 1× SDS–PAGE sample loading buffer (New England Biolabs # B7703S) and incubated at 98°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... IgG (Epredia™, NC748P) or 1X PBS was incubated with protein A beads (NEB, S1425S) with agitation for 1 hour at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The obtained bead-bound cDNA product then underwent Exonuclease I treatment (New England Biolabs; M0293M) to remove excess primer before proceeding with second strand synthesis.
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bioRxiv - Cell Biology 2022Quote: ... dynein coated beads were incubated with 5 μM SNAP-Cell TMR (New England BioLabs, #S9105S) in the column for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the binding reaction was added to 200 μl of 5% (v/v) amylose beads (NEB) that has been blocked overnight with 1 mg/ml BSA and 0.5 mg/ml tRNA in 20 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... The mRNA was purified using oligo-dT beads (New England Biolabs Japan Inc., Tokyo, Japan) and then the sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan Inc. ...
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bioRxiv - Microbiology 2022Quote: ... The beads were resuspended in 1X PBS containing NuPage LDS Sample Buffer (New England Biolabs) and dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... using the NEBNext Ultra II RNA Library Prep with Purification Beads Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences.
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bioRxiv - Developmental Biology 2023Quote: ... After the column cleanup a final beads cleanup was performed using AmpureXP (New England BioLabs) beads and the sample cDNA libraries were eluted with DNAse free water ...
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bioRxiv - Genomics 2022Quote: ... were enriched from total RNA using the SARS-CoV-2 / TBRV MagIC beads (ElementZero Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... Poly(A) + RNA was then isolated using double selection with poly-dT bead (E7490, NEB), and RNA-seq libraries were prepared using a NEB-Next Ultra II Directional Library Prep Kit for Illumina (E7760 ...
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bioRxiv - Immunology 2023Quote: ... beads were resuspended in 30 µL RNA ligation mix (1X RNA ligase I buffer (NEB), 1 µL RNA ligase I (NEB) ...
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bioRxiv - Genetics 2023Quote: ... Poly(A)+ RNA was further isolated using double selection with poly-dT beads (E7490, NEB). RNA-seq libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (E7760 ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins on glutathione beads were pre-treated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... a 0.6X clean-up was performed using the NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 20μL of 0.1X TE ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were eluted from beads with 11 μl 1x TE and 1.5 μl USER enzyme (NEB) for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were eluted from beads with 11 μl 1x TE and 1.5 μl USER enzyme (NEB) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... was ligated to the RNA fragments on bead using T4 RNA ligase 2 truncated KQ (NEB M0373 ...
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bioRxiv - Cancer Biology 2019Quote: ... Beads were first treated with T4 PNK (NEB, M0201L; 20 U in 80 μl reaction volume) in the absence of ATP (37°C ...
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bioRxiv - Genomics 2021Quote: ... The DNA library was purified with 1X volume of NEBNext® Sample Purification Beads (NEB #E7103) and eluted with 40 μL of 1 mM Tris buffer (pH 7.5).
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The complexes on the beads were incubated with 40 units T4 Polynucleotide kinase (New England Biolabs), first with P32 labelled ATP for 45 min ...
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bioRxiv - Genetics 2019Quote: ... we used the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB, Cat#E7103S). We basically followed the protocol from the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Genomics 2019Quote: ... Then the DNA on beads was used for PCR amplification with phusion DNA polymerase (NEB, M0530) and purified with VAHTS DNA Clean Beads to select the DNA fragments between 200bp and 600bp ...
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bioRxiv - Genetics 2019Quote: ... Libraries were end repaired on beads using the NEBNext Ultra End Repair module (New England Biolabs) and washed twice on 1x B&W (10 mM Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... Excess bead primers without RNA molecules were removed by the treatment of Exonuclease I (NEB, NEBM0293S). Then ...
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bioRxiv - Genetics 2020Quote: ... Messenger RNA (mRNA) was isolated from total RNA using NEBNext oligo dT beads (New England Biolabs). Isolated mRNA was fragmented and thereafter reverse transcribed to cDNA using NEBNext ultra RNA library preparation kit (New England Biolabs) ...