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Citations for New England Biolabs :
851 - 900 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Amylose pulldown assay was performed with Amylose Sepharose beads (New England BioLabs) in a pulldown buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... the beads were first treated with DNase I (New England Biolabs, M0303S) at 37 °C for 15 minutes and 1ml TRIzol (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the plate libraries were pooled together and AmpureXP beads (New England BioLabs) were used to purify the cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... 240 μl of oligo(dT) bead slurry (New England Biolabs, Frankfurt, Germany) were washed three times in 1 mL of lysis buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were incubated with 0.5 U/ μl T4 RNA ligase 1 (NEB) in 1x ligase buffer (containing 1 mM ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were treated with 10 μl of T4 Polynucleotide kinase (PNK; NEB) in 100 μl of 1X T4 PNK Reaction buffer containing 1 U/μl SUPERase•In™ RNase Inhibitor at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Biochemistry 2024Quote: ... Isolated MBP-tagged VPS26C was purified using Amylose beads (New England Biolabs) and eluted using 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... strand-specific RNA-seq libraries were constructed from submitted total RNA sample using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was isolated from the total RNA with an NEB Next poly(A) mRNA Magnetic Isolation Module Kit (New England Biolabs, MA, USA). RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were constructed from 100 ng and 1 μg total RNA using the NEBNext UltraTM Directional RNA Library Prep Kit for Illumina for use with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Hitchin, UK). mRNA was isolated ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA libraries for RNA sequencing were established from total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA, USA) to select poly-A mRNA followed by strand-specific library preparation using MGIEasy RNA Directional Library Prep Set V2.0 (MGITech Co ...
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bioRxiv - Immunology 2022Quote: B cells were cultured for 48 hours before total RNA was extracted using Quick-RNA™ Microprep Kit (ZymoResearch) and mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer’s instructions (Swift Biosciences) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg total RNA with RIN value above 7 was used for library preparation by Genewiz using NEBNext® UltraTM RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen) ...
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bioRxiv - Genomics 2020Quote: ... The RNA-Seq library was prepared with 100 ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, PN E7490S) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (PN E7760S) ...
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bioRxiv - Immunology 2020Quote: ... with Poly(A) mRNA Magnetic Isolation Module (CAT#E7490S) and index PCR primers (CAT #s E7335, E7500) (New England Biolabs; Ipswich MA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Poly(A) enrichment was performed using 500 ng of total RNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, USA) kit according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and libraries were constructed in technical triplicate using NEBNext Poly(A) Magnetic Isolation Module and NEBNext UltraII RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA), with size selection by AMPure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... The libraries were prepared from total RNA enriched for mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina (New England Biolabs, MA, USA) and sequenced on an Illumina NextSeq 500 instrument (Illumina ...
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bioRxiv - Genomics 2021Quote: ... 1000 ng samples of high quality total RNA (RIN≥ 7) was used for mRNA isolation using NEBNext Ploy(A) mRNA Magnetic Isolation module (New England Biolabs, catalog # E7490), followed by RNA library construction with NEBNext Ultra II Directional Lib Prep (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... Two hundred nanograms of total RNA was subsequently processed to generate RNA-seq libraries using NEBNext Poly(A) mRNA Magnetic Isolation and Ultra II Directional RNA Library Prep kit for Illumina (NEB E7490, E7760) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Total RNA extraction was followed by mRNA capture with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc.). For embryo and megagametophyte ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly(A)-enriched libraries for sequencing were then generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA). Paired-end sequencing was performed on the Illumina NextSeq instrument at the Harvard University Bauer Core ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified from 1 μg of total RNA using an NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (500 ng) was used to enrich the mRNA using NEBNext Poly (A) mRNA magnetic isolation module (New England Biolabs, Ipswich, USA) by following the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... 500 ng of total RNA was used as input for the NEBNext® Poly(A) mRNA Magnetic Isolation Module’ (#E7490L, New England Biolabs (NEB)) in accordance with the manufacturer’s standard instruction ...
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HTLV-1 Splice Sites in Prevalent Gene Vectors Cause Splicing Perturbations in Transgenic Human CellsbioRxiv - Genomics 2023Quote: ... One μg of total RNA was used to select polyadenylated RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, NEB). Half of the mRNA per sample was used for cDNA synthesis using the Superscript IV transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Approximately 1 ug of RNA was used as input to the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, catalog #E7490S), followed by NEB Ultra II RNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... 800 ng of RIN ≥7 of total RNA was used for library construction using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated from 50ng of total RNA (RIN value >7) using the NEBNext® Poly(A) mRNA magnetic isolation module, (New England BioLabs, Hitichin, Herts, UK) (NEB, #E7490) and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2023Quote: RNA-seq libraries were prepared from differentiated and undifferentiated cells of three biological replicates using NEBNext Poly(A) mRNA magnetic isolation module (NEB, Cat# E7490) for mRNA enrichment and Ultra II directional RNA Library Prep Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: 500 ng of total RNA per sample were utilized as input for mRNA enrichment procedure with NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490L, New England Biolabs, US) followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L ...
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bioRxiv - Genomics 2024Quote: ... RNA from each sample was enriched from 1 µg total RNA by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog #: E7490, New England Biolabs Inc.). RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog # ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Enlgand Biolabs, #E7760S/L).
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bioRxiv - Evolutionary Biology 2024Quote: ... 50-250 ng of total RNA were used for library production with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490), in conjunction with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... PolyA mRNA was enriched using the protocol of NEBNext ® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs #7490 S, USA). RNA sequencing libraries were then prepared using Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit (Yeasen ...
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bioRxiv - Immunology 2021Quote: ... the beads coated with cDNA were treated using Exonuclease I (Exo I, NEB) for 1h at 37°C with rotation to chew away any unbound mRNA capture probes ...
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bioRxiv - Genomics 2019Quote: ... Bead-nuclei were subsequently washed with 200 µL 1× RNA ligase buffer (NEB) supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads are then resuspended in Exonuclease I mix (New England Biolabs, Inc., M0293L) and incubate at 37°C for 50min ...
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bioRxiv - Biochemistry 2020Quote: ... bound RNA was eluted from the beads using Proteinase K (New England Biolabs) at 550C for 15 mins in modified Blob100 buffer (in the presence of 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
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bioRxiv - Immunology 2020Quote: ... Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... pH 8.0 and MBP fusions purified by incubation with amylose resin beads (NEB) and eluted with 50mM maltose in 50 mM Tris ...
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bioRxiv - Physiology 2020Quote: ... Excess bead primers were removed by the treatment of Exonuclease I (NEBM0293S, NEB), cDNA synthesis was performed using Template Switch Oligo (TSO) ...
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bioRxiv - Genomics 2021Quote: ... we removed fragments <150 bp using 1x AMPure® XP beads (NEB, UK). DNA quantification and quality control was performed using a BioAnalyser Instrument (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB #E6177)
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed with 500 μL of amylose resin beads (New England Biolabs) (prewashed with column binding buffer and resuspended to 50% slurry ...
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bioRxiv - Biochemistry 2022Quote: ... The enzyme complex was then further purified by incubation with chitin beads (NEB), to remove traces of TEV and fully active kinase or phosphatase ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...