Labshake search
Citations for New England Biolabs :
701 - 750 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... mRNA was purified from 1 μg of total RNA (≥100 ng/μL) using NEBNext Poly(A) mRNA Magnetic Isolation Module for Illumina (New England Biolabs). Sequencing libraries were generated using NEBNext Ultra II RNA Library Prep with Sample Purification Beads for Illumina (New England Biolabs ...
-
bioRxiv - Genetics 2019Quote: Poly-A RNA was extracted from 550 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used as input into the NEB Ultra II Directional RNA library kit for Illumina (NEB E7760 ...
-
bioRxiv - Developmental Biology 2020Quote: ... mRNA-seq libraries were prepared with the NEBNext Ultra RNA library preparation kit with poly(A) mRNA magnetic isolation module (NEB) essentially according to manufacturer protocols ...
-
bioRxiv - Systems Biology 2019Quote: ... RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). Sequencing was performed on an Illumina NextSeq 500 (75 cycles) ...
-
bioRxiv - Biochemistry 2020Quote: 1µg of total RNA from the polysome fractionated H9 cells was poly-a selected using the Poly(A) mRNA Magnetic Isolation Module (NEB). The mRNA was then adapter ligated and prepared for Illumina sequencing using the Ultra Directional RNA Library Prep Kit (NEB).
-
bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation module and NEB Ultra Directional RNA Library kit for Illumina (New England Biolabs).
-
bioRxiv - Plant Biology 2021Quote: ... RNA-Seq libraries were prepared from 1μg high quality RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μg of total RNA was used for polyA mRNA-selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), followed by library construction via NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Biophysics 2020Quote: The central part of the DNA construct used for magnetic tweezers was a 7.9 kbp fragment produced by restriction digestion of λ-DNA using SapI and BsaI (NEB) and subsequent agarose gel purification ...
-
bioRxiv - Cell Biology 2021Quote: ... double-stranded cDNA generation and library construction were performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L ...
-
bioRxiv - Cancer Biology 2022Quote: ... mRNA enrichment and library preparation was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep kit (NEB). Sequencing was done using the Illumina NextSeq500 to obtain >20 million 75bp single-end or 37bp paired-end reads per sample or at Genewiz (HiSeq ...
-
bioRxiv - Genomics 2022Quote: Polyadenylated mRNA was isolated from 1 μg of total liver RNA or from 1 μg of liver nuclear RNA from each treatment group (see below) using NEBNext Poly(A) mRNA Magnetic Isolation Module (cat. #E7490, New England Biolabs). The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (cat ...
-
bioRxiv - Genetics 2022Quote: We isolated mRNA from each total RNA sample using the 550 ng of total RNA input and the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). All samples were processed in a single batch and the isolated mRNA from each sample was used to prepare RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (NEB ...
-
bioRxiv - Microbiology 2022Quote: RNA library was assembled using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, New England Biolabs) as per manufacturer instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation module and NEB Ultra Directional RNA Library kit for Illumina (New England Biolabs). Library quality ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used as an input material for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). The libraries were multiplexed and paired-end sequenced (2 × 75 bp ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina in combination with the Poly(A) mRNA Magnetic Isolation Module (#E7760, #E7490, NEB) was used ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using 1µg RNA with the magnetic mRNA isolation module and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). DNA library was amplified by 7 PCR cycles and quality was analyzed using the fragment analyzer (Advanced Analytical) ...
-
bioRxiv - Cancer Biology 2020Quote: ... mRNA fraction was enriched using polyA capture from 200ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Next ...
-
bioRxiv - Cancer Biology 2019Quote: ... 75-bp stranded and paired-end poly-(A) RNA-seq libraries were prepared with NEBNext poly(A) mRNA magnetic isolation module (E7490L, New England BioLabs (NEB), Ipswich ...
-
bioRxiv - Cancer Biology 2019Quote: ... All RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (both from NEB). Library quality was analyzed using Agilent BioAnalyzer 2100 (Agilent ...
-
bioRxiv - Systems Biology 2019Quote: ... followed by library preparation using the NEBNext Poly(A) Magnetic Isolation Module and NEBNext Ultra RNA sequencing library prep kit for Illumina (NEB). At the time of RNA isolation for the clones ...
-
bioRxiv - Developmental Biology 2020Quote: Total RNA (1 μg) or TRAP-isolated RNA (200 ng) was subjected to the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) to isolate mRNA and proceeded directly to double stranded cDNA synthesis ...
-
bioRxiv - Microbiology 2021Quote: ... and poly-A selection to acquire fungal transcripts was done using the NEBNext Poly(A) mRNA magnetic isolation module (New England BioLabs). The remaining RNA from the co-cultured samples and the RNA from the axenic bacteria samples were depleted twice of bacterial rRNA using the Ribominus Transcriptome Isolation Kit for bacteria (Thermofisher) ...
-
bioRxiv - Physiology 2021Quote: ... Polyadenylated mRNA was purified from 50 ng of Total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490). Library preparation was completed on purified mRNA using the NEBNext Ultra Directional RNA Library prep kit (NEB ...
-
bioRxiv - Cell Biology 2019Quote: ... Messenger RNA (mRNA) was enriched from 1 μg of total RNA by Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Oligo dT-based mRNA isolation was carried out using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB; E7490) and libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... The NEBNext Ultra or Ultra II Directional RNA Library Prep Kit for Illumina with the mRNA magnetic isolation module (New England Biolabs) was used to prepare sequencing libraries from 1 μg of total RNA.
-
bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA with RIN >9.5 were used to prepare library using NEBNext poly(A) mRNA Magnetic Isolation Module and the NEB Next Ultra II RNA library prep kits for Illumina (New Engand Biolabs). Qubit High Sensitivity DNA kit (Life Technologies ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... mRNA libraries were constructed using the kits NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer protocol ...
-
bioRxiv - Bioengineering 2020Quote: ... and mRNA was isolated from ~1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
-
bioRxiv - Evolutionary Biology 2020Quote: Libraries for JU1373 and NIC58 were prepared simultaneously from mRNA isolated from 1 μg of pooled total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA fragmentation ...
-
bioRxiv - Microbiology 2020Quote: ... 1-2 μg of total RNA from each sample (or complete sample if the yield was lower) was used for mRNA isolation (Magnetic mRNA Isolation Kit, NEB). First strand cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System and a 1:1 mix of Oligo(dT ...
-
bioRxiv - Genomics 2020Quote: ... mRNA isolation was performed from 100 ng total RNA was done using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Exome sequencing library preparation was performed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... 1μg RNA from each sample was used for mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (E7490S, NEB). RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S ...
-
bioRxiv - Molecular Biology 2021Quote: ... EndoCβ-H1 and mouse islets was determined with an Agilent Bioanalyzer and mRNA enrichmed was performed from 500-1000ng of RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB). Library preparation from mRNA and from miRISC-isolated RNA was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were multiplexed using the NEBNext Multiplex Oligos for Illumina (NEB) ...
-
bioRxiv - Cell Biology 2022Quote: ... Mature mRNA was enriched from 400 ng of total RNA per sample using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Bioengineering 2022Quote: ... and mRNA was isolated from ∼1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
-
bioRxiv - Immunology 2022Quote: ... Library construction (input: 40 ng per CD8+SP sample) was performed using a NEBNext Poly(A) Magnetic Isolation Module (#E7490; NEB) followed by a NEBNext Ultra II Directional RNA library prep kit for Illumina (#E7760 and/or #E7770 with #E7765 ...
-
bioRxiv - Systems Biology 2022Quote: Library preparation was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: RNA libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Poly(A)+ RNA transcript was isolated from 1 ug purified total RNA (RNA integrity number >9.0) with NEBNext poly(A) mRNA magnetic isolation module (New England Biolabs #E7490). RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S ...
-
bioRxiv - Biochemistry 2022Quote: ... PolyA-selected libraries were made using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Zoology 2022Quote: ... where 250 nanograms of total RNA per tissue was used with the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Physiology 2022Quote: ... RNA-seq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit coupled with Poly(A) mRNA Magnetic Isolation Module and NEBNext multiplex oligos for Illumina (New England Biolabs). Deep sequencing was performed on the NextSeq 500 sequencing system (Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Plant Biology 2023Quote: ... 500 ng of total RNA was used for mRNA isolation using NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and the purified mRNA was used to construct libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...