Labshake search
Citations for New England Biolabs :
1101 - 1150 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... mRNA was isolated from purified 1 µg total RNA using oligo-dT beads (New England Biolabs, Ipswich, MA, US) and fragmented in reverse transcription buffer by incubating at 85 °C for 7 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was then end-repaired and poly(A) tailed on-bead by treatment first with T4 PNK (NEB) and then treatment with yeast poly(A ...
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bioRxiv - Genetics 2023Quote: ... The remaining beads were incubated with 1 ul RNasin (Fisher) and 0.5 µl of 20 mg/ml Proteinase K (NEB) at 42°C and 1200 rpm for 15 minutes in a thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were transferred to a tube and incubated with 10 μM SNAP-Cell TMR-Star dye (New England Biolabs) for 1 hour at 4°C prior to the TEV buffer washing step ...
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bioRxiv - Molecular Biology 2023Quote: ... the product was purified using magnetic SPRI beads and amplified using 0.5 µM i5/i7 Illumina indexed primers in Q5 High-Fidelity Master Mix (NEB).
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bioRxiv - Cell Biology 2023Quote: ... We prepared libraries using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (New England BioLabs). We indexed samples using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3 & Index Primers Set 4) ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed with Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB E7765) and sequenced on sequencing machine ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were eluted by mixing the beads with 21µl RNase H Elution Buffer and 4 µl RNase H (New England Biolabs) and incubated at 37 °C for 30 min while shaking ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was then extracted from washed beads using Trizol LS and treated with T4 Polynucleotide Kinase (New England Biolabs) to obtain 5’ phosphate ends for subsequent ligations and passed through NucAway columns (Ambion ...
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bioRxiv - Cell Biology 2024Quote: ... and TE buffer, DNA was reverse crosslinked by incubating beads in Elution buffer (1% SDS, 0.1M NaHCO3 and Proteinase K (NEB)) ...
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bioRxiv - Molecular Biology 2024Quote: ... a master mix containing hydrogel beads carrying DNA stub and ligation mixture was distributed in the same 4 x 96-well plates such that each well would contain 12.5 μl of close-packed hydrogel beads,1.25 µl of 10× ligation buffer (NEB, B0202S), 0.3 μl of T4 DNA ligase I (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... to 3 μM concentration in a total volume of 50 μl and mixed with 20 μl amylose beads (New England BioLabs). After mixing the proteins and the beads ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated and bead-purified in preparation for A-tailing using a custom NEB Paired-End Sample Prep Premix Kit (New England Biolabs). Illumina sequencing adapters were ligated for 15 minutes at 20°C and adapter-ligated products were then bead-purified and enriched with 10 cycles of PCR using primers containing a hexamer index that enables library pooling.
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bioRxiv - Cell Biology 2020Quote: ... Beads were resuspended in H2O used for library preparation with the NEBNext Ultra II Library Prep kit for Illumina (NEB). Beads were then washed 4 times using Tween wash buffer (5 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and end-repair processing were performed with the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs).
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bioRxiv - Developmental Biology 2020Quote: 500 ng of DNA attached to beads were end-repaired by incubating in 100 μl end-repair mix (1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were subjected to glass bead lysis in the presence of 1x phosphatase buffer mix (1x PMP buffer (New England Biolabs), 1x MnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were resuspended in Elution Buffer (Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs)) and the bound Enp1-TAP containing complexes were eluted for 1.5 – 2 hours ...
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bioRxiv - Genomics 2019Quote: ... 3’ P ends of RNA were dephosphorylated by resuspending bead-nuclei in 190 µL dephosphorylation solution (1× PNK buffer (NEB), 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was purified with AmpureXP beads and library was prepared with NEBNext Ultra II DNA Library Prep Kit Illumina (NEB) according to the protocol and sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries were generated using NEBNext Ultra II RNA Library Prep with Sample Purification Beads for Illumina (New England Biolabs) in accordance with the manufacturer’s recommendations and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... followed by purification with Ampure beads and mRNA synthesis with the HiScribe™ T7 ARCA mRNA Kit (Cat. E2065S, NEB). Then the in vitro transcribed mRNAs were treated with DNase I (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... and the soluble fraction of the lysate was passed through a chitin bead column (New England Biolabs, Ipswich, MA, USA), allowing the CusB fusion to bind to the resin via its chitin-binding domain ...
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bioRxiv - Plant Biology 2019Quote: ... Following purification with AMPure XP beads, the DNAs were ligated to the Barcode (EXP-PBC001, Nanopore) using LongAmp Taq (M0287S, NEB). The barcoded DNA library was then purified with AMPure XP beads ...
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bioRxiv - Biophysics 2019Quote: ... Both the 2 kb spacer and 1.5 kb biotin handle DNA were ligated to 5’-CGGT 1 µm polystyrene oligo beads overnight at 16 °C using T4 DNA ligase (NEB). The ligated beads were first washed with TE + 0.5 M KCl + 20 µg/mL β-casein ...
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bioRxiv - Genomics 2021Quote: ... end repair was carried out with a mixture of bead-immobilized T4 DNA polymerase and T4 polynucleotide kinase (both kind gifts of Dr. M. Xu, New England Biolabs) in a 20 µl volume of End Repair buffer ...
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with SPRI beads (0.9X) and quantified on a bioanalyzer and with NEB Illumina quant kit (NEB#E7630). HiC libraries were sequenced on a NextSeq500 either 150bp or 75bp paired-end reads.
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bioRxiv - Biochemistry 2020Quote: ... These beads were then resuspended in equal amounts of PBS+ DNaseI digestion buffer with 3 ul (6U) of DNAseI (M0303S, NEB) and incubated at 37°C for 40min - 1h and separated by centrifuging at 2000 rpm for 2 min ...
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bioRxiv - Microbiology 2021Quote: Protein bound glutathione Sepharose beads were resuspended in 1x NEBuffer™ for Protein Kinases supplemented with 1mM ATPγS and 10 units of CK2 (NEB) and incubated at 30°C for 45 minutes ...
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bioRxiv - Genomics 2022Quote: ... Then we performed the bead-on T7 transcription reaction by HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040S). The amplified RNAs were processed to the PCR-cDNA sequencing kit (nanopore) ...
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bioRxiv - Biophysics 2022Quote: ... GephE and its dimer-deficient mutant (GephEmm) were initially subjected to affinity chromatography on chitin agarose beads (New England Biolabs), followed by ion exchange chromatography and subsequent size exclusion chromatography.
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bioRxiv - Neuroscience 2020Quote: ... Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB), or with 100 microliters of lambda phosphatase assay buffer alone ...
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bioRxiv - Molecular Biology 2020Quote: ... the ligated DNA was subjected to a Fill-in reaction by resuspending the beads in 40 μL of 1X phi29 buffer (NEB) containing 0.2 mg/mL BSA (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was subjected to λ exonuclease digestion by incubating the beads with 50 μL of λ exonuclease reaction buffer (NEB) containing 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... using 10 μl of the bead suspension in a 50 μl reaction with NEBNext Ultra II Q5 2x master mix (NEB) and 0.5 μM each Solexa 1GA/1GB primers (Solexa 1GA ...
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bioRxiv - Microbiology 2020Quote: ... A-tails were added to the 3′ ends of the DNA by incubating the beads with Klenow fragment (exo-) (NEB) in a 100 μL reaction ...
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bioRxiv - Microbiology 2022Quote: ... Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB) and sequenced using Illumina Miseq v3 (150 cycles) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we added 150 – 1000 ng of DNA to 1 μl of prepared MBD2-Fc/protein A beads (New England Biolabs), then washed and eluted the DNA as described by Chiou and Bergey (2018) ...
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bioRxiv - Systems Biology 2019Quote: Bead purified DNA-RNA hybrid on beads were ligated at the anticodon nick by a combination of SplintR and T4 DNA ligases (NEB) to minimize ligation efficiency bias ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was submitted to mRNA Isolation and RNA fragmentation with NEBNext Oligo d(T)25 beads (NEW ENGLAND BioLabs) and cDNA synthesis ...
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bioRxiv - Cancer Biology 2020Quote: ... eluted in 8 μl and in vitro transcribed (with the beads) at 37 °C overnight for linear amplification using the T7 High Yield RNA polymerase IVT kit (NEB). Following IVT ...
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bioRxiv - Genomics 2020Quote: ... Double-stranded cDNAs were washed with XP Beads then were handled with NEBNext Ultra II End-prep enzyme mix (New England Biolabs). After an XP Bead-washing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sheared fragments were evenly distributed between 100 bp and 1500 bp and fragments of ~600 bp were selected using Agencourt AMPure XP Beads (New England Biolabs) following a protocol of double-size selection ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765S). Sequencing was carried out at the Max Planck Genome Centre (Cologne ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
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bioRxiv - Microbiology 2019Quote: ... meliloti 2011 strains containing the plasmid pRK-MS2-rnTrpL was performed using MS2-MBP fusion protein bound to amylose beads (New England Biolabs) as described by Smirnov et al ...
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bioRxiv - Biochemistry 2019Quote: ... 10µM of MBP-tagged Pat1 construct and 10µM Lsm1-7 were mixed and incubated at room temperature for 15 minutes before being added to pre-equilibrated amylose beads (New England Biolabs) for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... NEBNext adaptors with hairpin loop structure were ligated and library fragments were purified with SPRIselect sample purification beads (NEB, USA). After ensuring the quality of libraries on the Agilent Bioanalyzer 2100 system ...