Labshake search
Citations for New England Biolabs :
751 - 800 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: The phenol chloroform extracted RNA from previous step weas further purified to select for polyadenylated RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and reverse transcribed using SuperScript III reverse transcriptase (Invitrogen ...
-
bioRxiv - Plant Biology 2023Quote: ... Total RNA of 500 ng was used to enrich the mRNA using NEBNext Poly (A) mRNA magnetic isolation module (Catalog: E7490, New England Biolabs) by following the manufacturers’ protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Library preparation and selection of poly-adenylated transcripts were done using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT ...
-
bioRxiv - Genomics 2023Quote: Directional poly(A)+ RNA-Seq libraries were prepared using 300 ng of DNase-treated RNA using the Poly (A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA libraries were generated using 1µg RNA with the magnetic mRNA isolation module and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). DNA library was amplified by PCR and quality was analyzed using the fragment analyzer (Advanced Analytical) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... RNA library preparation was performed on total or polysomal RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions with 1 μg of RNA as a starting point ...
-
bioRxiv - Cancer Biology 2023Quote: RNA-Seq libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
-
bioRxiv - Cell Biology 2023Quote: ... Unbound material was removed by washing the samples 5x for 5min with head over head rotation at 4°C in wash buffer plus detergent (1x buffer XT, 1% digitonin, 2 mM PMSF and 10% glycerol) using a magnetic separator (New England Biolabs). Bound material was finally eluted with Biotin elution buffer (1x buffer Biotin XT elution ...
-
bioRxiv - Cell Biology 2023Quote: Poly(A)+ RNA was isolated from nuclei and whole cells (see C2C12 cell fractionation above) using the Magnetic mRNA Isolation Kit (New England Biolabs). Two rounds of binding ...
-
bioRxiv - Genomics 2023Quote: ... 100 ng of total RNA was used as input for mRNA selection and conversion to cDNA using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Preparation Kit for Illumina (New England BioLabs) ...
-
bioRxiv - Physiology 2023Quote: ... Libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
-
bioRxiv - Plant Biology 2023Quote: ... We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T) 25 magnetic isolation module (New England Biolabs), followed by first strand cDNA synthesis using NEBNext Ultra II RNA Library Prep Kit for Illumina according to the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... Then enrichment of Poly(A) mRNA was performed with the ‘NEBNext Poly(A) mRNA Magnetic Isolation Module’ (New England Biolabs). RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 100 ng of total intact RNA was used with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490S), and the enriched mRNA was prepped for sequencing with the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB ...
-
bioRxiv - Genomics 2023Quote: ... Library preparation has been done with 250ng of input RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
-
bioRxiv - Genomics 2023Quote: ... and the resulting libraries prepared for Illumina sequencing using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
-
Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... containing mRNA was isolated from the RNA extracted from the flow sorted samples using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... On-column DNase I digestion was performed followed by mRNA isolation with the NEBNext Poly (A) mRNA Magnetic Isolation Kit (NEB). Library preparation was done with the Ultra II Directional RNA Library Prep for Illumina following the manufacturer’s manual ...
-
bioRxiv - Developmental Biology 2024Quote: ... mRNA Magnetic Isolation Module (E7490L) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Immunology 2023Quote: ... PolyA mRNA was isolated using the NEBNext® Poly(A) mRNA magnetic isolation module (E7490, New England Biolabs, Ipswich, MA). RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S ...
-
bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Cell Biology 2024Quote: ... Libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
-
bioRxiv - Systems Biology 2024Quote: Approximately 100 ng of each RNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was used for standard library preparation using NEBNext PolyA mRNA magnetic isolation module (NEB, E7490). In brief ...
-
bioRxiv - Cancer Biology 2021Quote: Polyadenylated mRNA was isolated from 1 μg of total RNA from cultured tumor cells or excised tumor tissue using NEBNext® Poly(A) mRNA Magnetic Isolation Module (cat. #E7490, New England Biolabs) and the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Libraries for RNAseq were prepared by the Wellcome Trust Clinical Research Facility at Western General Hospital (Edinburgh, UK) using poly(A) mRNA magnetic isolation (NEB #E7490) and the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) ...
-
bioRxiv - Genetics 2021Quote: ... fragmented and primed the mRNA with NEB Next Poly (A) mRNA Magnetic Isolation Module (NEB #E7490, New England Biolabs, Ipswich, MA). The produced cDNA was quantitated with PicoGreen on a Perkin-Elmer Victor X3 fluorescence reader and kept at −80°C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We isolated poly-adenylated RNA from 1 ug of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and constructed sequencing libraries with the NEBNext Ultra II RNA Library Prep kit (NEB #E7770 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We purified mRNA from each total RNA sample using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and prepared sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep kit for Illumina with NEBNext® Poly(A) mRNA Magnetic Isolation Module (both New England BioLabs). Samples were adjusted to 400 ng total RNA and spiked with diluted 1/100 ERCC Spike-In Mix (4456740 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Poly-adenylated RNA was enriched from 1ug of RNA for each sample with the NEBNext PolyA mRNA Magnetic Isolation Module (NEB, E7490), incubated at 94°C for 15 min and double-strand cDNA was synthesized using SuperScript III reverse transcriptase (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 µg of total RNA per sample was enriched for poly-A using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB ...
-
bioRxiv - Genomics 2021Quote: ... 1μg of total RNA was used to enrich mRNA using NEB Magnetic mRNA isolation kit according to the manufacturer’s instructions (NEB, MA, USA). cDNA was synthesized and ligated to sequence adapters ...
-
bioRxiv - Neuroscience 2021Quote: ... were added to RNA samples (input RNA was 100ng per sample) prior to processing sample with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB # E7770 ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were prepared using with 1 μg of total RNA and mRNA isolation step was done by using NEBNext poly(A) mRNA Magnetic Isolation kit (New England Biolabs, E7490S). The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
-
bioRxiv - Plant Biology 2021Quote: ... and Poly(A) RNA purification was attempted using the NEBNext® Poly(A) magnetic isolation module (catalog number E7490, New England Biolabs). For sequencing of RNase R-treated samples ...
-
bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA libraries were made using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina using the established protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB#E7490). We used Rapid Run OBCG single read 50 bp on the Illumina Hi-Seq 2500 system to sequence these libraries with 2 technical replicates for each time-point and genotype ...
-
bioRxiv - Genomics 2020Quote: ... and the resulting libraries prepared for Illumina sequencing using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs: E7490) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
-
bioRxiv - Immunology 2021Quote: ... Total RNA (RIN>9.5) was used for mRNA isolation using the NEBNext Poly(A) mRNA Magnetic isolation module (NEB, Ipswich, MA). Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
-
bioRxiv - Neuroscience 2022Quote: ... were added to 100ng of RNA per sample prior to processing with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB # E7770 ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were constructed using the Swift Rapid Library Prep Kit (Swift Bio #R2096) with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: Messenger RNA was purified from total cytoplasmic and nuclear RNA samples using NEBNext Poly(A) nRNA Magnetic Isolation module (NEB; E7490S) with manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... Messenger RNA was enriched and separated from rRNA using the NEBNext®Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA library was prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2019Quote: ... 75-bp stranded and paired-end poly-(A) RNA-seq libraries were prepared with NEBNext poly(A) mRNA magnetic isolation module (E7490L, New England BioLabs (NEB), Ipswich ...
-
bioRxiv - Developmental Biology 2020Quote: ... Ribosomal RNA was removed and mRNA isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, E7490). During library preparation ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total RNA (1 μg) from each sample was used for library preparation with NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
-
bioRxiv - Microbiology 2021Quote: ... Polyadenylated mRNA was purified from 450 ng of total RNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490). Sequencing libraries were prepared with random primers using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Genetics 2020Quote: ... Poly-A RNA selection was done using 550 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490L), processing all samples in one batch ...