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Citations for New England Biolabs :
1151 - 1200 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... RAD fragments were enriched with a streptavidin bead pull-down and prepared as a sequencing library using a NEBNext Ultra DNA Library Prep Kit (New England Biolabs). Final libraries were sequenced using paired-end 150 bp sequencing on an Illumina HiSeq 4000 at Duke University's Center for Genomic sand Computational Biology sequencing facility.
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bioRxiv - Molecular Biology 2021Quote: ... beads were resuspended in GRB buffer and incubated with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070 ...
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bioRxiv - Molecular Biology 2022Quote: ... on-bead decapping and phosphorylation were performed in a 30 μl reaction with 5 units T4 PNK 3’ phosphatase minus (NEB), 2.25 μg GST-Dcp1-Edc1-Dcp2 ...
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bioRxiv - Cell Biology 2022Quote: ... the beads were resuspended in TEV buffer (final volume 5 mL) with 100 μL TEV protease (New England BioLabs, #P8112S) and incubated at 4°C on a roller overnight ...
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bioRxiv - Genomics 2022Quote: ... The DNA was then purified with AMPure XP beads (Beckmann Coulter) and ligated with sequencing adapters (ONT) using NEBNext Quick T4 DNA Ligase (NEB). After purification with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Beads were then dried before adding 50 µL DNaseI mix (3 U of DNaseI in 1X DNAse buffer (NEB, #M0303L) and incubated at 37 °C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... added to 200 μl of 2.5% (v/v) of MagStrep “type3” XT beads (IBA Lifescience) that has been blocked overnight with 1 mg/ml BSA (NEB) and 0.5 mg/ml tRNA (SigmaAlrich ...
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bioRxiv - Molecular Biology 2023Quote: ... increasing volumes of SPRI beads were mixed with 1 μl of a 4-fold dilution of 100 bp DNA ladder (New England Biolabs) and diluted to a final volume of 100 μl with 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... On-bead RNase H treatment control was performed for each reaction: washed beads were incubated with 300 µL 1X RNase H Buffer (NEB) plus 30 units of RNase H (NEB ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl reactions were incubated with 100 μl Ni-NTA beads equilibrated in 800 μl Ni-NTA buffer A with 10 mM imidazole and 40 U murine RNase inhibitor (NEB) at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and library prep performed on the beads using the NEBNext Ultra II DNA library prep kit for Illumina (NEB, E7645L), according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and prey (60 pmol for Retriever or 500 pmol for DENND10) were mixed with 20 µL of Amylose beads (New England Biolabs) in 1 mL of binding buffer [10 mM HEPES pH 7 ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was enriched from crude cell lysate in two rounds using biotinylated oligo-dT (IDT Europe) and streptavidin beads (New England Biolabs). RNA isolations were performed in batches in a randomized order ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent steps of RNA-seq were outsourced to Azenta who generated sequencing libraries from polyA-enriched RNA (captured with oligo-dT beads) using the NEBNext Ultra II RNA library prep kit for Illumina (NEB), multiplexed them ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide bound beads were washed with 100 µL 1x lambda phosphatase buffer prior to incubating with lambda protein phosphatase (P0753; NEB).
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bioRxiv - Immunology 2023Quote: ... was biotinylated with sulfosuccinimidyl-6-[biotinamido]-6-hexanamido hexanoate (sulfo-NHS-LC-LC biotin; ThermoScientific) and coupled to streptavidin beads (New England Biolabs). Patient samples were incubated with RBD-coupled beads and excess sera washed off with PBS (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... A-tailing and Illumina adaptors ligation were performed on the beads using NEBNext library preparation end-repair and A-tailing modules (NEB). Libraries were amplified and sequenced in biological replicates ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S). RNA-seq libraries for each sample type were prepared ...
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bioRxiv - Plant Biology 2023Quote: ... A color- balanced pair of two 3’-CLIP-RNA adapters was ligated on-bead to the RNAs in each sample using T4 RNA ligase 1 (New England Biolabs), with the ligation reaction incubated for 75 minutes at 21°C ...
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bioRxiv - Molecular Biology 2023Quote: ... nascent RNA was isolated with streptavidin beads and barcoded adapters ligated at 3’ ends of the nascent RNA (T4 RNA ligase 1 enzyme, M0204L; NEB) overnight at 25° C ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified DNA was then size enriched using 0.9X volume SPRI beads and subject to another round of PCR using 10x cycles with NEBNext Ultra II (NEB M0544S) using primers pairs cTF383+cTF399 for the promoter and oSA021+oSA023 for the gene body to add Illumina Read1 and Read2 overhangs ...
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bioRxiv - Neuroscience 2023Quote: RNA was released from the beads through protein K lysis (Monarch® Total RNA Miniprep Kit; New England Biolabs, #T2010S). After lysis ...
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bioRxiv - Molecular Biology 2023Quote: The miRCat-33 3’ linker was ligated to the 3’ end of the RNAs on the Ni-NTA beads with 800 units of T4 RNA ligase 2 truncated K227Q (New England Biolabs) in 1 x PNK buffer / 16.67% PEG 8000 in the presence of 80 units RNasin in a total volume of 80 µl ...
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bioRxiv - Microbiology 2023Quote: ... Double stranded on-bead DNA was digested with a mix of 3 blunt cutting enzymes (SspI-HF, StuI, and HincII) (NEB) to generate blunt-end DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... IP samples underwent on-bead ligation of barcoded RNA adapters (/5phos/rArGrArUrCrGrGrArArGrArGrCrGrUrCrGrUrG/3SpC3/) to the 3’ end using T4 RNA ligase (New England Biolabs). Following elution ...
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bioRxiv - Genomics 2024Quote: ... All subsequent steps were performed on that bead-bound DNA fraction including DNA ends repair with repair mix (10Х ligase NEB buffer ...
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bioRxiv - Biochemistry 2023Quote: ... custom-made rabbit polyclonal anti-AcK142 or anti-AcK226 (Ez Biolabs) Abs were incubated overnight at 4 °C with the lysate from mock vs ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Genomics 2020Quote: ... the beads were resuspended in 100uL of the following: 88 μL 1X NEB T4 DNA ligase buffer (New England Biolabs, B0202), 2 μL of 25mM dNTP mix ...
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bioRxiv - Genomics 2022Quote: ... The DNA was eluted from AMPure XP beads with 80 μL elution buffer and digested with the restriction enzymes 2 μL MluCI (NEB, R0538L) and 2 μL NlaIII (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... were prepared using the NEBNext® Ultra II FS DNA Library Prep Kit for Illumina with NEBNext Sample Purification Beads (New England BioLabs). This protocol includes a short PCR amplification (5-7 cycles ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were washed three times with IP buffer and resuspended in 90 ul of IP buffer with proteinase K (NEB, P8107S) for 30 mins at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We prepared libraries using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (New England BioLabs, Ipswich, Massachusetts). Final fragment sizes and concentrations were confirmed using a TapeStation 2200 system (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: For the oligo-dT affinity chromatography 0.5 ml of C1 or P2 fractions were incubated with an equal volume of a 50% slurry of oligo(dT) beads (NEB #S1408S) in Phy buffer ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The mRNA was then isolated through two rounds of poly-A selection using the oligo-d(T)25 beads (NEB #S1419S). The RNA was digested with nuclease P1 (Wako USA #145-08221 ...
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bioRxiv - Genetics 2020Quote: ... We then eluted the DNA from the beads using 10 μl of water and added to it 25 μl NEBNext HiFi 2x PCR MasterMix (NEB M0541), with 2.5 uL of each of the dual-indexed Illumina Nextera primers (25 μM) ...
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bioRxiv - Cell Biology 2021Quote: ... The 5’ RNA linker (5’-blocked and containing a 10 nt UMI (/5AmMC6/GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNNNN3’) was ligated to the RNA fragments on bead using T4 RNA ligase (NEB M0437) for 2 h at 24°C with shaking ...
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bioRxiv - Genomics 2022Quote: ... blunt DNA fragments on the beads were adenine-tailed by adding 7μl of Klenow 3’→5’ exo-polymerase 5U/μl (New England Biolabs cat. #M0212L), 2.3μl of dATP 10mM and 5 μl NEB2 of 10x NEBuffer 2 and incubating the mixture 30 minutes at 37ºC and a further 10 minutes at 65ºC to inactivate the enzyme.
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bioRxiv - Genetics 2022Quote: ... and DNA library preparation was performed using NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads Kit (NEB, #E6177). Whole-genome sequencing was performed using an Illumina NextSeq instrument ...
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bioRxiv - Genomics 2022Quote: ... The RNA was removed from beads by Trizol and then followed with the 3’ adapter ligation using T4 RNA Ligase 1 (NEB M0204L). After this ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries for next-generation sequencing were prepared using the NEBNext Ultra(tm) II Directional RNA Library Prep kit with Sample Purification Beads (NEB, E7765S). Libraries were indexed using NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 2 (E7335S and E7500S) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were next resuspended in 1X Thermo Pol Buffer and treated with 2 µL RNA 5’ Pyrophosphohydrolase (New England Biolabs M0356) at 37°C for 1 h to promote decapping of 5’ RNA ends ...
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bioRxiv - Genomics 2023Quote: ... Hi-C library was purified by adding 80 μl Ampure beads and amplified in two 100 μl pre-amplification reactions (50 μl 2x NEBNEXT Ultra II Q5 Master Mix (NEB, M0544L), 5 μl 10 μM Nextera-P5-pre-Primer ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (E7775S) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs E7600S). Library quality was assessed with the Agilent High Sensitivity DNA Kit (Agilent 5067-4626 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA) and the concentration determined by Bradford assay ...
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bioRxiv - Immunology 2023Quote: ... libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (New England Biolabs) according to the provided instructions ...
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bioRxiv - Genetics 2023Quote: ... Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775). Singular specific primers for the libraries sequencing on Singular which had the S1/S2 handles instead of Illumina’s p7/p5 handles.
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bioRxiv - Biochemistry 2023Quote: ... The DNA bound to the beads was made ss by λ exonuclease digestion of the strand with 5’ phosphate (5 U/mg DNA, NEB). The reaction was carried out at 370 C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (E7765; New England Biolabs, Ipswitch, MA). cDNA libraries were then indexed using the NEBNext Dual Index Kit (E7600S ...