Labshake search
Citations for New England Biolabs :
9051 - 9100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µL LongAmp Taq DNA Polymerase (NEB, # M0323L), 0.5 µM of each primer (First PCR F ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µL LongAmp Taq DNA Polymerase (NEB, # M0323L), 0.5 µM of each primer (Second PCR F UMI ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1µL of T4 ligase (NEB), 2.5µL 10mM ATP (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5’-hydroxyl (5’HO-RNA30-FAM-3’) or 5’-Gppp (5’Gppp-RNA30-FAM-3’) in 1x NEBuffer 3 (NEB; B7003), in 20% denaturing polyacrylamide gels ...
-
bioRxiv - Molecular Biology 2023Quote: ... RppH (NEB) treatment was performed with a starting amount of 500ng.
-
bioRxiv - Molecular Biology 2023Quote: ... Utilizing the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... digoxigenin-11-ddUTP was added to the 3’ end of the digested fragments using terminal transferase enzyme (New England Biolabs, Ipswich, MA, USA).
-
bioRxiv - Molecular Biology 2023Quote: ... truncated KQ (New England Biolabs), rRNA-depleted by a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat ...
-
bioRxiv - Molecular Biology 2023Quote: ... and amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs). DNA libraries were sequenced on HiSeq 4000 (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNAs were cleaved using RNAse H (New England Biolabs, Ipswich, MA, USA) with LA121 as a specific primer for HIS3-tCYC1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the second linker SI183 5’-/Phos/NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG/ddC/-3’ was preadenylated by Mth RNA Ligase (New England Biolabs) as described previously8 ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed with the Phusion High-Fidelity DNA polymerase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... prepared using the HiScribe T7 ARCA mRNA Kit (NEB, E2060). The translation reactions were incubated at 30°C for 40 mins ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli Poly(A) polymerase (NEB, M0276L) in the presence of 1 mM ATP and 1 unit/uL RNasin® (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... the NEBNext second strand synthesis kit (NEB, E6111) was used according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... mRNA was then extracted using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S) and RNA-seq libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... The NEBNext Ultra II End Repair/dA-tailing Module (NEB, E7546S) was used before ligating Nanopore native barcodes (EXP-NBD104&114) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and RNA-seq libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, E7420L). RNA-seq libraries were sequenced on an Illumina NextSeq500 Instrument with at least 10 million reads per library ...
-
bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR reagents (NEB, E3005) and the primers listed in Table S3.
-
bioRxiv - Molecular Biology 2023Quote: ... Murine (New England Biolabs [NEB]), using the Multi-beads Shocker at 3000 rpm for 30 sec for 2 cycles (Yasui Kikai) ...
-
bioRxiv - Molecular Biology 2023Quote: ... MYH7 fragment containing mutation was amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) and 500 nM forward and reverse primers (Table 3) ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated using the Monarch Total RNA Miniprep Kit (New England BioLabs, cat #T2010) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and BlpI (New England Biolabs) restriction sites (Integrated DNA Technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and sgRNA fragments were isolated by SbfI (New England Biolabs) restriction digestion and Ampure bead size selection then amplified by PCR for deep sequencing as described in 53 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 ul of micrococcal nuclease (Biolabs M02475, 2×106 U/ml) was added to 200 μl buffer containing 1 mM CaCl2 and incubated for 10 min 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... and reverse transcribed by ProtoScript II Reverse Transcriptase (New England Biolabs). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to linkers by T4 RNA Ligase 2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by phenol-chloroform extraction and processed according to the NEB Ultra II Directional RNA Sample Prep Kit (New England Biolabs, #E7760S).
-
bioRxiv - Molecular Biology 2023Quote: Individual sgRNA expression vectors were cloned by first annealing complementary synthetic oligonucleotide sequences containing each sgRNA protospacer (Table S3) flanked by BstXI (New England Biolabs) and BlpI (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... an ‘A’ base was added to the 3’ end of the blunt-end phosphorylated DNA fragments using the polymerase activity of Klenow (Exo-Minus) polymerase (NEB); Illumina genomic adapters were ligated to the A-tailed DNA fragments ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting PCR product was integrated in the pMV261 (81) plasmid digested with AatII and EcoRI using the Gibson Assembly strategy according to the manufacturer protocol (NEB).
-
bioRxiv - Microbiology 2023Quote: ... FloA-SNAP was labelled with 0.2 μM SNAP-Cell® TMR-Star (New England Biolabs, #S9105S) during the last 20 min of main culture incubation ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2.5µg of WTFL and NLucFL or 1.5µg of SGR purified genomic DNA was linearized with 1.5µL of SbfI-HF® (New England Biolabs #R3642L) in 1 × CutSmart buffer at 37ºC for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). Illumina MiSeq sequencing was carried out on the IlluminaHiseq2500 platform (Illumina Inc. ...
-
bioRxiv - Molecular Biology 2023Quote: ... 55µL rATP (NEB), 110µL nuclease-free water ...
-
bioRxiv - Molecular Biology 2023Quote: ... 110µL of resuspended nuclei was mixed with 55µL 10x T4PNK Buffer (NEB), 55µL rATP (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μl 10 mM MnCl2 buffer and 0 or 2 μl lambda phosphatase (NEB #P0753S, USA) in 38 μl supernatant ...
-
bioRxiv - Microbiology 2023Quote: The N-glycans of ACE2 were removed by PNGase F glycosidase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
-
bioRxiv - Microbiology 2023Quote: ... coli strain NEB5alpha (#C2987H, New England Biolabs) cultured overnight aerobically at 37°C in LB broth or solid LB agar ...
-
bioRxiv - Microbiology 2023Quote: ... assembled them using a NEBridge Golden Gate Assembly Kit (BsmBI-v2) (#E1602S, New England Biolabs), and then electroporated 1 μL of the reaction into electrocompetent NEB5alpha ...
-
bioRxiv - Microbiology 2023Quote: ... The upstream and downstream PCR products were designed to have an overlapping region of sequence to promote 3-part Gibson assembly (NEB Gibson assembly kit) and primers 5b and 4 had extensions to anneal to vector pMP62 ...
-
bioRxiv - Microbiology 2023Quote: ... The cloning was done using the NEBuilder HiFi DNA assembly cloning kit (NEB). Plasmids were transformed into E ...
-
bioRxiv - Microbiology 2023Quote: ... performed to exchange the previous gRNA sequence was achieved using a Q5 mutagenesis kit (New England Biolabs, Ipswich, Massachusetts, USA). gRNA sequences were inserted into pTREX-n-Cas9 using primers specific to the gene of interest in Supplementary Table 4 ...
-
bioRxiv - Microbiology 2023Quote: ... was used to amplify a 440 bp fragment of the coronavirus RDRP gene using Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs cat no: M0493S). PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel ...
-
bioRxiv - Microbiology 2023Quote: ... the isolated glycoproteins are treated by α-2,3/6/8 neuraminidase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 20 U murine RNase inhibitor (New England Biolabs) were added to each reaction ...
-
bioRxiv - Microbiology 2023Quote: ... PYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-PYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
-
bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 10 min and 2 μL of 20 mg/ml proteinase K (New England Biolabs, Cat. #P8107S) were added ...