Labshake search
Citations for New England Biolabs :
8951 - 9000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... Oligos included 5’ overhangs complementary to the sticky ends generated by BsaI-HF (NEB, R3733S) restriction enzyme ...
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bioRxiv - Microbiology 2023Quote: ... and treated with Exonuclease I (NEB). The abundances of reference never-edited ...
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bioRxiv - Microbiology 2023Quote: ... template DNA was digested with 25 units of EcoRI (New England Biolabs, Ipswich, MA). After droplet generation ...
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bioRxiv - Microbiology 2023Quote: ... PCR product was assembled with EcoRI-linearized pBAD L24 using NEBuilder HiFi DNA Assemly Master Mix (NEB). Strep-Tag II and His-tag C-terminally modified SAMase coding plasmids were constructed in a similar manner ...
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bioRxiv - Microbiology 2023Quote: ... II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C ...
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bioRxiv - Microbiology 2023Quote: ... All mutations in pBAD T3 SAMase were introduced using Q5 Site-Directed Mutagenesis Kit or KLD Master Mix (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Q5 High-Fidelity 2X Master Mix (New England Biolabs) was used for all PCR reactions.
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bioRxiv - Microbiology 2023Quote: Routine PCR was performed using Q5 High-fidelity Mastermix (NEB M0492L). PCR purification and gel extraction was performed using Macherey-Nagel Kit cat ...
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bioRxiv - Microbiology 2023Quote: ... Q5 High Fidelity DNA Polymerase (New England Biolabs, United States) was used for end point PCR to determine specificity of the PaeRPB2f/r primers (Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA was extracted using the Total RNA isolation kit (#T2010, NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and a 1:1 mix of oligodT and random hexamer primers to obtain cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μg tissue for each sample using the Total RNA isolation kit (#T2010, NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and random hexamer primers to obtain 50ng/μl cDNAs.
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were performed with Q5 DNA polymerase (NEB, MA, USA) and assembly was performed using the HiFi DNA assembly master mix (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and assembly was performed using the HiFi DNA assembly master mix (NEB). The digested assembly reaction was amplified by rolling circle amplification using the Repli-g mini kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Infectious RNA was generated by in vitro transcription using the ARCA 2X T7 master mix (NEB) with subsequent transfection in 293T cells (ATCC® CRL-3216™ ...
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bioRxiv - Microbiology 2023Quote: ... Verified p2NIL/ΔsecA2 plasmids were digested with PacI (NEB; 37 °C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: Verified amplicons were combined with the pMV306H integrative vector backbone at a ratio of 1:1 v/v and allowed to ligate overnight at 4°C with T4 DNA ligase (NEB). Ligation products were subsequently transformed into chemically competent DH5ɑ E.coli ...
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bioRxiv - Microbiology 2023Quote: Total RNA from cell pellets was extracted using a Monarch Total RNA Miniprep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was extracted from cell pellets using Monarch Genomic DNA Purification Kits (New England Biolabs) and viral episome copies quantified by qPCR of the viral gene ORF57 as described in the qRT-PCR method.
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed on 500 ng of total RNA using a LunaScript™ RT SuperMix Kit (New England Biolabs) as according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and then were incubated with linearized pGL3-promoter vector and the Gibson Assembly Kit reagents (NEB). Reactions were then transformed into competent Top10 E ...
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bioRxiv - Microbiology 2023Quote: ... and BamHI-HF (NEB, Cat# R3136L) using In-Fusion Snap Assembly Master Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 100 ng RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (#57760S and #57765S, New England Biolabs, Frankfurt am Main, Germany) with the NEBNext® Poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs) and SPRIselect beads (#B23319 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were linearized with the AscI enzyme (New England Biolabs, #R0558S) for SINV and NotI enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... coli cells (New England Biolabs, #C2987H) were transformed with the eluted plasmid DNA according to the manufacturer’s instructions and grown on LB broth selection plates ...
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bioRxiv - Microbiology 2023Quote: ... for SINV and NotI enzyme (New England Biolabs, Cat. #R0189S) for ONNV in 0.05-ml reactions ...
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bioRxiv - Microbiology 2023Quote: ... These two linker sequences were connected to both termini of replicon DNA by ligation using 1,000 units of T4 DNA ligase [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... using NEBuilder HiFi DNA assembly master mix (NEB). Plasmid sequence was confirmed via Sanger sequencing (Molecular Biology Shared Resources ...
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bioRxiv - Microbiology 2023Quote: ... pACYC184 was linearized by BamHI and HindIII digestion (New England Biolabs, Ipswitch, MA). The locus for arnD ...
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bioRxiv - Microbiology 2023Quote: ... coli TOP10 (New England Biolabs), and constructs were confirmed using full length plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: ... The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs). The Gibson or ligated product was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... Gibson assembly was performed using the generated PCR products and linearized pACYC184 according to the manufacturer’s protocol with HiFi DNA Assembly Master Mix (New England Biolabs). The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Microbiology 2023Quote: ... 200 μM each dNTP (NEB), 1 U Phusion DNA polymerase (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...
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bioRxiv - Microbiology 2023Quote: ... 1 U Phusion DNA polymerase (NEB), and 10 ng template DNA ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR for SARS-CoV-2 from cell lysates was performed using the TaqMan assay for the CDC N1 gene primers and probes from Integrated DNA Technologies (catalog no. 10006600; Integrated DNA Technologies) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) as previously described (6) ...
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bioRxiv - Immunology 2023Quote: ... digested with BamHI (NEB, R3136S) and KpnI (NEB ...
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bioRxiv - Immunology 2023Quote: ... or Gibson Assembly Master Mix (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Klenow fragment (NEB) and adaptor ligated with annealed adaptors (Nimblegen Systems) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM vanadyl ribonucleoside complex (NEB, S1402S). The cells were then washed 4 times in wash buffer containing 25% formamide (Roche ...
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bioRxiv - Microbiology 2023Quote: ... containing 1x NEBuffer 2 (NEB, B7002S), 1 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... Double-stranded DNA was synthesized with DNA Polymerase I (New England BioLabs, M0209S) and subsequently fragmented by dsDNA Fragmentase (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of RNase Inhibitor (New England BioLabs, M0314), and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs ...