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Citations for New England Biolabs :
9201 - 9250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Plasmid construction was performed by: PCR amplification of the fragment to insert in the plasmid with Q5® High-Fidelity DNA Polymerase (New England Biolabs), digestion with the appropriate FastDigest restriction enzymes (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Substrates were transcribed in vitro from purified PCR products using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... After cleaning the plasmid digestion using the Monarch DNA Cleanup Kit (NEB, #T1030), the DNA concentration of the linearized plasmid was measured to determine the number of DNA molecules in the stock solution ...
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bioRxiv - Microbiology 2023Quote: ... Pooled genomic DNA samples were used to sequence libraries constructed using the NEBNext Ultra II Library Prep Kit (NEB, Ipswich, USA). Paired-end metagenomics sequencing was performed on an Illumina NextSeq 550 sequencer using NextSeq High Output Kit v2 sequencing reagent kit ...
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bioRxiv - Microbiology 2023Quote: ... and NheI-HF (NEB, Cat# R3131M) using In-Fusion Snap Assembly Master Mix (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180, NEB), and Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning was performed by assembling the purified PCR fragments into the specified pET derivative expression vector using the commercially available NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Antarctic phosphatase (New England Biolabs) and polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2023Quote: ... followed by mRNA purification using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S). RNA-seq library was constructed using NEBNext Ultra II RNA-library preparation kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using Monarch® Total RNA Miniprep Kits (New England Biolabs) as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then loaded onto an 8% polyacrylamide gel alongside a 50 bp ladder (New England Biolabs) and resolved at 100 V for 45 minutes in TBE buffer ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Thermostable RNase H (New England Biolabs, USA) was added ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® with the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) were used to generate DNA libraries for sequencing (New England Biolabs, USA) as per the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... dephosphorylated with antarctic phosphatase (NEB; 37 °C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... gene marker cassette by overnight ligation at 4 °C with T4 DNA Ligase (NEB). Overnight ligations were transformed into chemically competent DH5ɑ E ...
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bioRxiv - Microbiology 2023Quote: ... 300 bp-insert RNA-seq Illumina libraries were constructed using ∼1.0 μg of enriched mRNA that was fragmented then used for synthesis of strand-specific cDNA using the NEBnext Ultra Directional RNA Library Prep Kit (NEB-E7420L). The cDNA was purified between enzymatic reactions and the size selection of the library performed with AMPure SpriSelect Beads (Beckman Coulter Genomics) ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High Fidelity polymerase with HF buffer and 3% DMSO (NEB) under the following cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenic PCR constructs were generated by amplifying the upstream and downstream DNA fragments flanking the gene(s) of interest followed by fusion of these fragments with the Janus Cassette using a HiFi assembly master mix (NEB). Transformation of TIGR4 with the mutagenic construct (100ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was amplified in triplicate 25 μl reactions consisting of primers at final concentrations of 0.2 μM and 1X PCR reagent (Q5® Hot Start High-Fidelity 2X Master Mix, New England BioLabs, UK). Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881 ...
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bioRxiv - Microbiology 2023Quote: ... The Q5 Site-Directed Mutagenesis Kit protocol (New England Biolabs) was used for site-directed mutagenesis on previously generated vectors ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (NEB™) was added to a final concentration of 1X per reaction and incubated at 37°C for an additional 15 min ...
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bioRxiv - Microbiology 2023Quote: ... AvcI was then purified using Monarch® RNA Cleanup Kit (NEB™). Purity of the product was evaluated using a 1.0% TBE agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... The AvcI DNA template for in vitro transcription was PCR amplified from pAvcI using Q5 High-Fidelity DNA Polymerase (NEB™). To incorporate the T7 promoter into the final AvcI DNA template ...
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bioRxiv - Microbiology 2023Quote: ... or Q5 polymerase (New England BioLabs).
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bioRxiv - Microbiology 2023Quote: ... or Q5 polymerase (New England BioLabs). Each fragment consisted of an antibiotic resistance cassette (kanamycin or gentamicin resistance genes encoded by aph and aacC1 ...
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bioRxiv - Microbiology 2023Quote: ... the pABA plasmid (from E. coli INV110) was methylated with Dam methylase (New England BioLabs), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The two flanking regions of the chosen genomic exchange region were amplified and joined with PCR fragment carrying antibiotic resistance cassette by overlapping PCR using Q5 polymerase (New England BioLabs). Next ...
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bioRxiv - Microbiology 2023Quote: ... was performed with enzymes and buffers from New England Biolabs® (NEB, Ipswich, MA, USA) according to respective protocols ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of sample was incubated with 1.25 μL of proteinase K (NEB P8107S) for 1 hour at 55°C then 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Microbiology 2023Quote: ... the Phusion DNA polymerase (New England Biolabs) was used ...
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bioRxiv - Microbiology 2023Quote: ... and a 1848 bp fragment (containing the 1131 bp Pfcytb open reading frame) was amplified with primers (SI Appendix, Fig. S2A,B) and Phusion DNA polymerase (NEB). PCR product was verified by gel electrophoresis as single band of predicted size ...
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bioRxiv - Microbiology 2023Quote: ... we used the NEBNext Ultra II End Repair/dATailing module (E7546S, NEB, USA) to prepare 1000 ng DNA from each sample ...
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bioRxiv - Microbiology 2023Quote: HTS was performed on the three RNA extracts after a step of rRNA depletion with the rRNA depletion kit (NEB, Evry, France), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... 1x UDG buffer (NEB), and 1.75 U RNase A at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of RNase inhibitor and 3 μl of T4 RNA ligase 2 (truncated) (New England Biolabs) were added and mix well by pipetting ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μl of T4 RNA ligase (New England Biolabs) and mixed ...
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bioRxiv - Microbiology 2023Quote: ... and polynucleotide kinase (PNK) (New England Biolabs) were sequentially applied on the samples ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using Monarch® Total RNA Miniprep Kits (New England Biolabs) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 μl of Phusion high-fidelity DNA polymerase (New England Biolabs), then reached to final reaction volume of 50 μl by addition of 26.5 μl of Nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared from an equal amount of RNA by reverse transcription using NEB mMuLV reverse transcriptase (New England Biolabs, MA, USA). The list of primers used for amplification is shown in supplementary table 2 (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (NEB; 18 U), and lysozyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... upon restriction with HindIII and XbaI (NEB). The resulting construct was called pybtP:GFP28 ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Microbiology 2023Quote: Mutagenesis vectors were constructed by Gibson Assembly (New England Biolabs) on the pSEVA612S backbone and were maintained in E ...
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bioRxiv - Microbiology 2023Quote: Protein expression vector pMAL-c5Xa (NEB) was used as the backbone for constructing the mCherry reporter ...
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bioRxiv - Microbiology 2023Quote: ... coli cells using the manufacturer’s protocol (NEB, C3019). The cells were plated on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... predigested with NotI-HF (NEB, Cat# R3189L) and BamHI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression of NTCP, inserts encoding NTCP cDNA were cloned into a pCAGGS vector (Niwa et al., 1991) predigested with EcoRI-HF (NEB, Cat# R3101M) and NheI-HF (NEB ...