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Citations for New England Biolabs :
8901 - 8950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... was subsequently introduced into the His- MBP-TEV-BARD126-777 vector by Gibson assembly in frame between the TEV site and BARD1 (37) according to manufacturer’s instructions (New England BioLabs).
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabsⓇ Inc., MA, USA) was preferred in order to select aggregation inhibitory peptide selection ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were sequenced on an Illumina NovaSeq 6000 sequencer.
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by ligation with T4DNA ligase (NEB). The 3′UTR of METTL3 was amplified using primers with Not I sites and cloned into CX plasmid as described earlier (Bukhari et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were conducted using the Q5® High-Fidelity DNA polymerase system (New England BioLabs, Ipswich, MA); 5 μL 5X Q5 Reaction Buffer,0.25 μL Q5 High Fidelity DNA Polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Microbiology 2023Quote: ... psfx94-3xFlag was constructed from psfx94 using primer pair pAH56_3xFlag.F/94sfx_3xFlag.R and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µL of phosphate saline buffer (PBS) and labeled by incubation with SNAP-cell TMR-Star (New England Biolabs) for 30 min at 37°C in the dark at a final concentration of 250 nM ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of the sRNA-enriched library was done with NEBNext Ultra II RNA kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s recommendation which included cDNA synthesis ...
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bioRxiv - Microbiology 2023Quote: ... the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) was used ...
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bioRxiv - Microbiology 2023Quote: ... NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) was used to assemble plasmids ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB beta cells (New England BioLabs) and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB beta cells (New England BioLabs) and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... To this extent the OneTaq® DNA Polymerase kit (New England BioLabs inc.) was adopted ...
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bioRxiv - Plant Biology 2023Quote: ... coli DH5α strain (New England Biolabs) and plated on LB medium containing 25 µg/ml Kanamycin ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid DNA of positive clones was prepared and checked by restriction digest using HincII for HvST1/Hvst1 and BamHI and BglII (New England Biolabs) for HvASY1 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was then amplified using nested PCR (Primers in Table S3) with High-Fidelity Phusion or Q5 DNA polymerase (New England Biolabs), and the PCR products ligated into pGEM®-T Easy vector (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... UAS expression constructs were generated by combining the linearized pGW-HA.attB backbone and each individual ZIKV fragment in Gibson assembly reactions (NEB Gibson Assembly Cloning Kit, E5510S). To generate NS4A::2K ...
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bioRxiv - Cell Biology 2023Quote: ... fragmented DNA was treated with RNAse-H (NEB) for 4-6 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 1-102-syn and S129A-syn) were created within this pcDNA vector by site directed mutagenesis using Phusion High-Fidelity DNA Polymerase (New England Biolabs)1 ...
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bioRxiv - Neuroscience 2023Quote: ... coli (NEB) and isolated using Qiagen Midi-kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Stereo-seq chip was washed with NF-H2O supplemented with 0.05 U/ul RNase inhibitor (NEB, M0314L). Six sections from the anterior half endostyle were mounted onto a single Stereo-seq chip with 3-minute incubation on a Thermocycler Adaptor at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was digested with DNase I (NEB) and cDNA was synthesized ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Microbiology 2023Quote: ... Q5 High-Fidelity 2X and OneTaqTM 2X Master Mix (New England Biolabs; Ipswich, MA, USA) were used according to the manufacturer’s instructions for PCR amplification during gene cloning and colony PCR to confirm gene cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was synthesized from 1 μg of RNA using M-MuLV Reverse Transcriptase (NEB) and random hexamer primer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested cells were rinsed with ice-cold PBS and resuspended in lysis buffer with 1% Triton X-100 and 40 U/mL murine (New England Biolabs) for 20 minutes with intermittent tapping on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by labeling with γ-P32-ATP and T4 polynucleotide kinase (Cat#: M0201L, NEB). The reactions were further processed and digested with P1 Nuclease (Cat# ...
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bioRxiv - Molecular Biology 2023Quote: ... after Micrococcal nuclease (NEB) treatment to remove RNA and DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 500Lng of RNA was digested with RNase If and RNase T2 (Cat#: M0243L, New England Biolabs/NEB and Cat# ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were further processed and digested with P1 Nuclease (Cat#: M0660S, NEB). In vitro-transcribed 4B mRNA (firefly luciferase reporter (Bukhari et al. ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA was then DNase treated (DNase I, NEB) and processed through another Trizol extraction to purify the example of enzyme ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNAs were treated with Exonuclease I (NEB, M0293L) at 37°C for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... between KpnI (NEB CAT#: R3142L) and EcoRI (NEB CAT# R3101L ...
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bioRxiv - Physiology 2023Quote: ... and EcoRI (NEB CAT# R3101L) restriction enzyme sites ...
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bioRxiv - Plant Biology 2023Quote: ... in the presence of Murine RNase inhibitor (NEB), following NEB protocol (#M0368) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.),1.25 μl Chai Green Dye 20X (Catalog No ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.4 U/μL Murine RNAse Inhibitor (Catalog No. M0314S, New England BioLabs). Reactions were incubated for 8 hours at 37°C in a thermocycler ...
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bioRxiv - Synthetic Biology 2023Quote: ... The supernatant was removed and treated with DpnI to degrade template plasmid DNA (Catalog No. R0176S, New England BioLabs Inc.). The mixture was incubated at 37°C for 15 minutes (T100 Thermal Cycler ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (500 μg) (Catalog No. T2050L, New England BioLabs) and concentration was measured using a NanoDrop ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
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bioRxiv - Physiology 2023Quote: ... Samples were DNAse treated (B0303s/ M0303s, NEB) and RNA concentrations were measured with the Nanodrop ...
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bioRxiv - Biochemistry 2023Quote: ... EcoRV digestion (NEB), and ion exchange of the excised fragment by HiTrap DEAE (147-bp ‘601’ ...
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bioRxiv - Plant Biology 2023Quote: ... and ligated using T4 ligase (NEB) with previously digested (FastDigest Eco31I ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... or H4 (New England BioLabs, Catalog # M2504S) respectively was added to the mixture at a final concentration of 0.1 mg/mL ...
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bioRxiv - Genomics 2023Quote: ... Nuclei lysis buffer consists of NST-DAPI buffer with 0.1U/μl RNase Inhibitor (NEB #M0314L)1 ...
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bioRxiv - Genomics 2023Quote: ... Sequencing library was constructed using the NEB Ultra library preparation kit (New England Biolabs, Ipswitch).
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bioRxiv - Microbiology 2023Quote: ... 5 μL 20 μM Cas9 (New England BioLabs), and 5 μL of 40 μM transcribed dgRNAs ...
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bioRxiv - Microbiology 2023Quote: ... 1 μL of proteinase K (New England Biolabs) was added ...