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Citations for New England Biolabs :
9301 - 9350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... per manufacturer ’s instructions (NEB). Library quality was assessed by Fragment Analyzer NGS (Agilent) ...
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bioRxiv - Developmental Biology 2023Quote: ... 50μM dNTPs and 0.1U of Bst3.0 (NEB) or 0.8U of MRT (ThermoScientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... then poly(A) RNA was extracted using the Magnetic mRNA Isolation Kit (NEB). Immunoprecipitation of methylated RNA (MeRIP ...
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bioRxiv - Developmental Biology 2023Quote: ... and then ligated using T4 DNA ligase (NEB, M0202S) to obtain the plasmid pAW63-Tex10 ...
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bioRxiv - Developmental Biology 2023Quote: ... and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were digested with Cac8I (R0579, New England Biolabs), which cuts the wildtype allele ...
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bioRxiv - Developmental Biology 2023Quote: ... transcribed with T7 polymerase (E2040S, New England Biolabs) and purified using mirVana miRNA isolation kit (AM1560 ...
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bioRxiv - Cell Biology 2023Quote: Libraries were prepared on an automated liquid handler (Biomek i7) using the NEBNext Ultra II DNA library preparation kit (NEB, E7645), according to the manufacturer’s instructions and without size selection ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl of 10× CutSmart buffer (NEB), 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei ...
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bioRxiv - Immunology 2023Quote: ... Digested bovine serum albumin (New England Biolabs cat # P8108S) was analysed between each sample to avoid sample carryover and to assure stability of the instrument and QCloud (Chiva et al. ...
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bioRxiv - Cell Biology 2023Quote: ... NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) was used to prepare libraries for further sequencing according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... coli (NEB). Rabbit polyclonal antibodies were raised against the purified polypeptides.
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bioRxiv - Cell Biology 2023Quote: ... 500ng of total RNA were used for RNA-Seq library preparation with the NEBNext Ultra II RNA Library Prep Illumina Kit (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center within the NYUAD Core Technology Platform) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and mutated by PCR to generate the disease variant alleles (New England Biolabs #E0554S). The utilized mutagenic primers are as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... 13.5% PEG8000(NEB) was added to the RT solution ...
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bioRxiv - Developmental Biology 2023Quote: ... after digesting the plasmid with BsmBI (NEB). The sgRNA was transcribed from the BamHI linearized pT7-gRNA vector using the MEGAshortscript T7 kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...
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bioRxiv - Immunology 2023Quote: ... total RNA was reverse transcribed into cDNA with LunaScript RT Supermix (New England Biolabs, E3010) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and second-strand synthesis performed with Klenow fragment (NEB M0212L) to enhance transcript recovery in the event of failed template switching 14 ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB 5-alpha (New England Biolabs) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: ... In vitro Xrn-1 digests were performed with 1U Xrn-1 (NEB), 10U RppH (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Gibson assembly was performed with the NEB Gibson Assembly® Master Mix (New England Biolabs) with a vector-to-insert ratio of 1:5 ...
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bioRxiv - Microbiology 2023Quote: ... or Phusion (NEB) enzymes ...
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bioRxiv - Microbiology 2023Quote: ... and ligations were performed with T4 DNA ligase (New England Biolabs). All cloning steps were performed in either E ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... DNase I (New England Biolabs, USA) was used to remove leftover DNA oligonucleotides and Agencourt® AMPure® XP magnetic beads (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthsised using using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesised using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass pool ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq library was constructed using NEBNext Ultra II RNA-library preparation kit (NEB, E7770S) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... pCW-lic was dephosphorylated with antarctic phosphatase (NEB, M0289) to prevent self-ligation ...
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bioRxiv - Immunology 2023Quote: ... 3000 ng of high molecular weight DNA was prepared by blocking available DNA ends with calf intestinal phosphatase (NEB Cat #M0525). DNA and dA-tails on all available DNA ends were cleaved by RNPs and Taq polymerase (New England Biosciences Cat #M0273 ...
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bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Neuroscience 2023Quote: ... the constructs were ligated to the pET25 vector in separate reactions using T4 DNA Ligase (New England Biolabs, catalog #M0202) overnight at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 µL of 5x Phusion HF Buffer (NEB #B0518S), and 5 µL of 2.5 mM dNTPs (Takara #4025) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.5 µL of Phusion High-fidelity DNA Polymerase (NEB #M0530), 10 µL of 5x Phusion HF Buffer (NEB #B0518S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified fragment was then subjected to EcoRI-HF (NEB #R3101S) and XbaI (NEB #R0145S ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in SOC medium (NEB #B9020) at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5 µL of 10x T4 DNA Ligase Buffer (NEB #B0202). The reaction samples were incubated at room temperature for two hours and purified by FastGene PCR/Gel Extraction Kit (Nippon Genetics #FG-91302) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.2 µL of Phusion High-Fidelity DNA Polymerase (NEB #M0530), 4 µL of Phusion HF Buffer (NEB #B0518S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.6 µL of 100% DMSO (NEB #12611P), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4 µL of Phusion HF Buffer (NEB #B0518S), 2 µL of 2 mM dNTPs (Takara #4025) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.6 µL of 2.5 mM dNTPs (NEB #N0447), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (NEB #C2527I), and the plasmids for the Blasticidin and Zeocin resistance marker-based systems were introduced to T7 Express chemically competent cells (NEB #C2566I ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.64 µL of Phusion High-Fidelity DNA Polymerase (NEB #M0530S), 6.4 µL of Phusion HF Buffer (NEB #B0518S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in 1 mL of SOC medium (NEB #B9020S) at 37°C ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Each variant was amplified with primers PK421&422 using Q5 High-Fidelity 2X Master Mix (NEB) in a 200 µl reaction followed by DpnI digestion for 2 h ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... An insert downstream of aga2 was amplified from pYD2 using primers PK463+PK464 in a 1 ml PCR reaction (Q5 High-Fidelity 2X Master Mix, NEB), DpnI digested for 2 h and SPRI purified ...
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bioRxiv - Synthetic Biology 2023Quote: ... AMX sequencing adaptors were ligated by mixing 2.5 ul of the assembly mix with 5 ul AMX and 5 ul Blunt/TA mastermix from NEB and incubated for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: The bacterial source strains used for this work (Table S3) were Listeria monocytogenes LL195 as a main infection model and Escherichia coli NEB5α (New England BioLabs) for protein purification ...