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Citations for New England Biolabs :
9251 - 9300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The plasmid was first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High-Fidelity DNA Polymerase (New England Biolabs), a forward primer binding to the spliced leader sequence and a reverse binding to the VSG 3’untranslated region ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis with ProtoScript II First Strand cDNA Synthesis (New England Biolabs). The sequences were then amplified ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High-Fidelity DNA Polymerase (New England Biolabs), a forward primer binding to the spliced leader sequence and a reverse binding to the VSG 3’ untranslated region ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of sample was incubated with 1.25 μL of proteinase K (NEB P8107S) for 1 hr at 55°C then 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... The eluted gp120 coree protein was deglycosylated overnight in a 37 °C water bath with Endoglycosidase Hf (New England Biolabs). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... the lentiCRISPRv2 vector was digested with BsmBI (NEB), gel purified using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 0.02% RNase free bovine serum albumin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Immunology 2023Quote: ... and KpnI (NEB, R3142S), using the In-Fusion kit (Takara ...
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bioRxiv - Immunology 2023Quote: Genomic DNA corresponding to selected and diversity control samples were PCR amplified with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) as described in20 ...
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bioRxiv - Immunology 2023Quote: ... Successful ligation was confirmed by Sanger sequencing of DH5a (NEB) colonies transformed with the ligation reaction ...
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bioRxiv - Immunology 2023Quote: ... using Gibson assembly (New England Biolabs). After verification of the correct sequence ...
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bioRxiv - Immunology 2023Quote: ... The multiplex products were used as templates to the second PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs Inc., M0530L) that barcodes each well/perturbation separately ...
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bioRxiv - Immunology 2023Quote: Phage DNA was subjected to two rounds of PCR using Q5 High-Fidelity 2X Mastermix (NEB), as previously described (Garrett et al. ...
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bioRxiv - Microbiology 2023Quote: ... Proteinase K (NEB) and incubated at 56 degrees C overnight ...
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bioRxiv - Immunology 2023Quote: ... Library preparation was done using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: ... 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs, Ipswich, MA, USA) according to the recommendations of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... 1μg of NotI-linearized plasmid was subjected to in vitro transcription using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, Ipswich, MA, USA). For use in ELISpot ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Semi-quantitative PCR was performed using Taq DNA polymerase with standard Taq buffer (New England BioLabs Cat # M0273S). PCR products were analyzed on 2% Agarose gels with 0.5 ng/L Ethidium bromide using a 1kb Plus DNA Ladder (New England BioLabs Cat # N3200S ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The washed pellet was resuspended in TM2 buffer with 1 mM CaCl2 and 12000 U of MNase (New England Biolabs), incubated at 23°C for 15 minutes and the reactions stopped by addition of 0.5 mM EGTA ...
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bioRxiv - Genomics 2023Quote: ... run and qPCR with NEBNext Library Quant kit (New England Biolabs) respectively ...
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bioRxiv - Genomics 2023Quote: ... and 1x CutSmart buffer (NEB) at 37 °C for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... 3 µL 10x NEBuffer 4 (NEB), 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP ...
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bioRxiv - Genomics 2023Quote: ... nick ligation was then performed in a 30 µL reaction with 3 µL of 10x rCutSmart Buffer (NEB), 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+ ...
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bioRxiv - Genomics 2023Quote: ... 9.6 U Bst 2.0 (NEB), 9.6 U Bst 3.0 (NEB) ...
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bioRxiv - Genomics 2023Quote: ... in a 70 µl reaction with 1x CutSmart buffer (NEB) for 20 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 1 mM ATP (NEB), 4 mM DTT (Promega) ...
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bioRxiv - Genomics 2023Quote: ... 9.6 U Bst 3.0 (NEB), or 9 U Isopol SD+ (ArcticZymes) ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Genomics 2023Quote: ... and 7.5 U of Klenow fragment 3’→5’ exo-(NEB), except for input DNA that is degraded (e.g. ...
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bioRxiv - Genomics 2023Quote: Input DNA was fragmented with 0.14 U/µL of Hpy166II restriction enzyme (NEB) in a 70 µl reaction with 1x CutSmart buffer (NEB ...
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bioRxiv - Genomics 2023Quote: ... 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+) (NEB), and 15U of E ...
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bioRxiv - Genomics 2023Quote: ... 170-200 ng of library was indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The manufacturer’s protocol was followed with the following deviations ...
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bioRxiv - Genomics 2023Quote: ... pOPO374 was linearized with primer introduced ends through PCR by Q5 polymerase (NEB). The resulting PCR product was then circularized by treatment with PNK (NEB ...
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bioRxiv - Genomics 2023Quote: ... The resulting PCR product was then circularized by treatment with PNK (NEB) and T4 ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... and T4 ligase (NEB) in an overnight reaction at 16C.
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bioRxiv - Genomics 2023Quote: ... DNA was digested in situ with MboI (NEB) and Csp6I (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA from single adult male flies was extracted using using the Monarch Genomic DNA Purification Kit (New England Biolabs), using a protocol we developed (dx.doi.org/10.17504/protocols.io.bp2l694qklqe/ v1 ...
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bioRxiv - Genetics 2023Quote: ... 2 μL of genomic DNA prep was fragmented by restriction digest in 20 μL reactions with EcoRV-HF and HinDIII-HF (New England Biolabs) for 2h ...
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bioRxiv - Genetics 2023Quote: ... 1 ng of oligoarray library DNA was PCR amplified for 12 cycles in 40 μL reactions using Q5 High-Fidelity DNA Polymerase (NEB) and primers specific to the right or left end library ...
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bioRxiv - Genetics 2023Quote: ... 5 U StuI (NEB), CutSmart buffer (NEB) ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Genetics 2023Quote: ... After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646, NEB, MA, USA), 1nL mixture was micro-injected into single-cell embryos ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Genetics 2023Quote: ... and ligated into pWS1728 (BsrGI and AflII digested) using high concentration T4 DNA ligase (NEB M0202T). Ligations were transformed into DH5⍺ E ...
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bioRxiv - Genetics 2023Quote: The yeast plasmids recovered after thermoselection were subject to a shortened PCR with 15 cycles with oligonucleotide pairs oWS1408 (5°-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAGCATATAATCCCTGCTTTA-3°) and oWS1409 (5°-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCCAGGGGTGGTGCAAACTATG-3°) using a high-fidelity Q5 polymerase (New England Biolabs, Ipswich, MA) to attach overhang sequences ...