Labshake search
Citations for New England Biolabs :
9001 - 9050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... coli (NEB Cat#: C3040H) were transformed and plated on LB Agar (Fisher BioReagents Cat# ...
-
bioRxiv - Microbiology 2023Quote: ... coli MG1655 and DH10β (NEB) strains and all molecular biology was performed as previously described38 ...
-
bioRxiv - Microbiology 2023Quote: ... USA] or the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
-
bioRxiv - Microbiology 2023Quote: ... TTP and IFN-λ1 were cloned using primers found in supplementary table 1 and inserted between pCW57-MCS1-2A-MCS2 EcoRI (NEB # R3101S) and BamHI (NEB # R3136S ...
-
bioRxiv - Microbiology 2023Quote: ... the allelic lacI-containing shuttle vector pMQ123 was first digested with BamHI (New England BioLabs) and the targeted small regulatory RNAs were amplified using Phusion polymerase (New England BioLabs ...
-
bioRxiv - Microbiology 2023Quote: ... plus 30 units of RNase H (NEB) or 15 µL nuclease-free water at 37°C for 3 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... rTg4510 mouse brain sections were incubated in the absence (control) or presence (treated) of 10,000 U/mL λ phosphatase (New England Biolabs, cat. no. P0753) for 24 hours at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... and in vitro transcribed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... The LT3-GFP-T7-PGK-Neomycin vector was digested with XhoI and EcoRI-HF (New England Biolabs). An shRNA targeting the Renilla luciferase (Ren713 ...
-
bioRxiv - Microbiology 2023Quote: ... 0.375 uL BbsI (NEB), 0.125 uL T4 Ligase (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... The nuclei were digested with the first enzyme (CviQI (NEB, R0639L) or NlaIII (NEB ...
-
bioRxiv - Genomics 2023Quote: ... we performed PCR with Q5 Hot Start High-Fidelity (NEB M0494S), column purification with QIAquick PCR Purification Kit (Qiagen 28104) ...
-
bioRxiv - Genomics 2023Quote: First stand cDNA synthesis and cDNA amplification were performed with a NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs, MA, USA) and Iso-Seq Express Oligo Kit (Pacific Biosciences (PacBio) ...
-
bioRxiv - Genetics 2023Quote: ... coli (NEB) to yield a worm pellet of ∼2 mL ...
-
bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from individual embryos by heat shock denaturation in 50 mM NaOH (95°C for 20 minutes) and used for PCR by using Q5 High-Fidelity Taq Polymerase (New England Biolabs). The presence of specific amplicons was tested by running the PCR product on 1% agarose gel ...
-
bioRxiv - Genetics 2023Quote: ... seven independent reactions were set up where the EcoRI (NEB), PsiI (NEB ...
-
bioRxiv - Genetics 2023Quote: ... Plasmid assembly was conducted using NEBuilder HiFi DNA Assembly cloning kit (NEB, cat # E5520S) and transformed to DH5α-competent cells ...
-
bioRxiv - Genetics 2023Quote: ... Library preparation was performed using the NEBNext Ultra II FS kit (NEB, E7805L) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2023Quote: ... The remaining beads were incubated with 1 ul RNasin (Fisher) and 0.5 µl of 20 mg/ml Proteinase K (NEB) at 42°C and 1200 rpm for 15 minutes in a thermomixer ...
-
bioRxiv - Immunology 2023Quote: ... followed by ligation (T4 DNA ligase NEB M0202L) at 16℃overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Approximately 500Lng of RNA was digested with RNase If and RNase T2 (Cat#: M0243L, New England Biolabs/NEB and Cat# ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Rec J Exonuclease (NEB), and cDNA was generated with EpiScript RT enzyme (Lucigen ...
-
bioRxiv - Cancer Biology 2023Quote: ... The linearized vector and the sgRNAs heteroduplexes were ligated overnight at 16°C using T4 DNA ligase (NEB), and the ligation product was used to transform One Shot™ Stbl3™ cells (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
-
bioRxiv - Biophysics 2023Quote: ... was supplied in a final concentration of 2.04ng/μL (for each rung) and the 100-1000bp ladder (100bp DNA Ladder, New England BioLabs) in a final concentration of 71.4ng/μL (for all rungs ...
-
bioRxiv - Biophysics 2023Quote: ... Purified SNAP-CP was incubated with 5x excess of SNAP-surface-549 dye or SNAP-surface-649 dye (New England Biolabs, USA) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: RNA-Seq Libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB). The RNA-Seq libraries were quantified by Qubit and qPCR according to the Illumina Sequencing Library qPCR Quantification Guide and the quality of the libraries was evaluated on Agilent Bioanalyzer 2100 using the Agilent DNA-1000 chip ...
-
bioRxiv - Developmental Biology 2023Quote: ... -phospho-histone H3 (1:300, New England BioLabs), -c-Fos (1:1000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... building parallel total-RNA and poly(A)+ Illumina strand-specific libraries using the Ultra II RNA-seq library kit (NEB #E7765) (see below) ...
-
bioRxiv - Cell Biology 2023Quote: ... and 25 μU ml-1 apyrase (NEB)] ...
-
bioRxiv - Neuroscience 2023Quote: ... The full-length PCR product was purified using Monarch Gel Extraction Kit (NEB #T1020), according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... in 1x Cas9 Nuclease Reaction Buffer (NEB #B0386A) (total volume 3 µl ...
-
bioRxiv - Neuroscience 2023Quote: ... The PCR reaction was performed using Phusion High-Fidelity DNA Polymerase (NEB #M0530) with 10 µM of each primer (synthesized by IDT ...
-
bioRxiv - Plant Biology 2023Quote: ... coli competent cells (NEB) by electroporation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Q5 DNA polymerase (NEB, USA) was used for DNA amplification containing an 80 bp overlapped segment with the adjacent fragments ...
-
bioRxiv - Plant Biology 2023Quote: ... mRNA Magnetic Isolation Module and used to construct RNA-seq libraries with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s protocol with slight modifications ...
-
bioRxiv - Plant Biology 2023Quote: ... The MBP-OsTLP protein or the MBP control protein was bound to amylose resin (New England Biolabs, Beverley, MA, USA), and then the LssaCA-His protein was added to the beads ...
-
bioRxiv - Molecular Biology 2023Quote: ... and HiFi Assembly (New England Biolabs, Frankfurt am Main, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated at 42°C overnight with the addition of 3 μl of β-agarase (NEB). The DNA mix was then gently poured into combing reservoirs containing 1.2 ml MES and the genomic DNA was combed onto salinized coverslips (Genomic Vision ...
-
bioRxiv - Molecular Biology 2023Quote: ... Site-directed mutagenesis of DmelOrco was done by PCR with the Q5® site directed mutagenesis kit (NEB) using primers optimized with the NEBase Changer online tool and following the supplier’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: Libraries were prepared for high-throughput sequencing according to manufacturer’s recommendations using the Ultra II DNA Library Prep kit (NEB). Samples were multiplexed and paired-end sequencing was performed using either a MiniSeq or NovaSeq 6000 (Illumina).
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were circularized with T4 kinase (NEB, M0201L) and T4 ligase (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was mixed with 5 U of T4 PNK (NEB), DNA splint (0.4 pmol ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 U/μL RNase Inhibitor (NEB) in reaction buffer (50 mM Tris-HCl pH7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The reverse-transcription (RT) reactions were performed with 2X LunaScript® RT SuperMix Kit (NEB) with 5 μL of RNA input in 10 μL total volume ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA cleavage products were purified using Monarch RNA Cleanup kit (NEB) and used for a one-pot reaction of end-prep and splint annealing ...
-
bioRxiv - Systems Biology 2023Quote: ... 25 μL of the NEBNext Q5 HotStart HiFi PCR Master Mix (NEB, M0543S), and nuclease-free water to a final volume of 50 μL ...
-
bioRxiv - Plant Biology 2023Quote: ... The mRNA was enriched with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, #E7490). The library was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...