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Citations for New England Biolabs :
8801 - 8850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... These amplicons were inserted into pMQ30 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into miniTn7/pMQ56 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... Library preparation was performed using the Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... The coding sequence of the libraries was amplified using the Q5 Hot Start High-Fidelity DNA Polymerase (NEB) and the PCR amplicons were separated from primer dimers on a 0.5x TAE 1 % agarose gel ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) (New England Biolabs) culture in animal-free TB media ...
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bioRxiv - Bioengineering 2023Quote: ... Restriction enzymes and T4 DNA ligase were obtained from NEB and Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA transformed into competent cells (NEB, C2987H).
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bioRxiv - Cell Biology 2023Quote: ... The resulted PCR product co-transformed with pEGFP-C1-2µ-URA3 cut with BamHI (NEB, R3136) into yeast strain BY4743 (EUROSCARF Y20000 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained product was then cut with NheI (NEB, R3131) and EcoRI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The IMS-BirA construct and PCR products from ER-and PO-BirA products were cut with HindIII (NEB, R3104) and BamHI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... following manufacturer’s instructions and transformed into competent cells (NEB, C2987H).
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bioRxiv - Cell Biology 2023Quote: ... and BamHI (NEB, R3136) and cloned into pcDNA™5/FRT/TO (TermoFischer ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... ligated with the cut backbone and transformed into competent cells (NEB, C2987H). EGFP-YgiM(TA ...
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bioRxiv - Cell Biology 2023Quote: ... and EcoRI(NEB, R3101). The large DNA fragment was gel purified (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... digested with BbsI-HF enzyme (NEB, # R3539S). DNA sequence ...
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bioRxiv - Cell Biology 2023Quote: ... 40 U/µL MNase (NEB, M0247S), 0.2 U/μL EcoRI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 uL of 2xphusion PCR mastermix (Phusion High-Fidelity PCR Master Mix with HF Buffer – 100 rxns, NEB, cat #M0531S), 0.625uL of 100uM F primer ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were prepared with an NEBnext DNA Library Prep kit (NEB, E7370L). Adaptor-ligated DNA was size-selected and purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... cpTK-Cluc (New England Biolabs, Ipswich, MA, USA), pBluescript (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 µg/µl BSA (NEB, B9000 S). Immunoprecipitations were performed at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... followed by in vitro transcription with the T7 High Yield RNA Synthesis kit (NEB). Newly synthesized sgRNAs were purified using the MEGAclear kit (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: ... dephosphorylated by quick calf intestinal phosphatase (NEB Inc.) and heat-inactivated ...
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bioRxiv - Genetics 2023Quote: ... were ligated overnight at 20°C with a mix of six 5’ phosphorylated telorette oligonucleotides (0.1 µM each) and 2,000 units of T4 ligase (NEB Inc.) in a 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... NCPF 8977 (aggregating) and NCPF 13029 (weakly-aggregating) using Monarch RNA extraction kit (NEB cat no. T2010) as per manufacturer’s specification ...
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bioRxiv - Microbiology 2023Quote: ... the vector miniTn7/pMQ56 was first digested with SmaI (New England BioLabs) and the fleQ gene and its native promoter were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then subjected to DNase treatment to remove any residual genomic DNA using Monarch RNA purification kit (NEB cat no. 2040S).
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bioRxiv - Microbiology 2023Quote: ... the allelic exchange vector pMQ30 was first digested with SmaI (New England BioLabs) and ∼1.5kb flanking regions of the targeted gene were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... and ProtoScript II first strand cDNA synthesis kit (New England BioLabs) using Oligo-p(dT)15 or d(T)23 VN primers ...
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bioRxiv - Microbiology 2023Quote: ... and BamHI (NEB # R3136S) restriction sites.
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1% NP-40 buffer as above and 20 µg of lysate or 50 µL of supernatants were subjected to Endo H (P0702S NEB) or PNGase F (P0709S NEB ...
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bioRxiv - Microbiology 2023Quote: ... or PNGase F (P0709S NEB) digestion following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... the XhoI site in the original construct was replaced with an MluI site using Q5 site directed mutagenesis (New England Biolabs) using primers ...
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bioRxiv - Microbiology 2023Quote: ... A set of Gibson Assembly (New England Biolabs, Inc.) primer pairs (RicT-F2 and RicT-R2 ...
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bioRxiv - Microbiology 2023Quote: ... and cloned into pTM1 (a pDONR/Zeo based plasmid) downstream of the ACT1 promoter using Gibson cloning kit (NEB), and then introduced into pDG33 using Gateway cloning (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Libraries for RNAseq were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). Individual libraries were uniquely barcoded with NEBNext Multiplex Oligos for Illumina sequencing platform (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and sequenced on the Illumina NovaSeq 6000 Sequencer (2×150 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using Monarch PCR/DNA Cleanup Kit (New England BioLabs). ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... or the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs, Ipswich, MA) (corpuscles samples) ...
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bioRxiv - Genetics 2023Quote: PCR was performed using OneTaq polymerase (NEB #M0480). Reactions consisted of 1X OneTaq Standard Reaction Buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (E7600, New England BioLabs, Ipswich, MA), home-made SPRI beads (Rohland and Reich 2012 ...
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bioRxiv - Cell Biology 2023Quote: ... using Gibson Cloning kit (New England Biolabs). The protocol is available here dx.doi.org/10.17504/protocols.io.j8nlkkzo6l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... for GST fusion proteins or amylose resin (New England Biolabs #E8021S) for MBP fusion proteins for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The mixtures were diluted into 400 µl of Binding Buffer and then incubated with the appropriate resin (amylose for MBP, New England Biolabs # E8021S ...
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bioRxiv - Cell Biology 2023Quote: ... All sequences were inserted into L4440 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into DH5a bacteria ...
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bioRxiv - Microbiology 2023Quote: ... Amplification of DNA was done using Q5 High-Fidelity DNA polymerase (NEB) and clones obtained were confirmed by sequence analysis.
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bioRxiv - Microbiology 2023Quote: ... Each fragment was amplified by PCR using the Q5® High-Fidelity DNA Polymerase (NEB, Cat #: M4091L) following the manufacturers protocols ...
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bioRxiv - Microbiology 2023Quote: Reporter fusions of sRNA targets were in-vitro translated in the absence and presence of sRNA using the PURExpressⓇ In Vitro Protein Synthesis kit (NEB). Four picomoles of in-vitro transcribed 5’UTR reporters (including the RBS/sRNA interaction site and the first 10 codons of flgM ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were dephosphorylated with Antarctic Phosphatase (NEB), 5’ end-labeled (γ32P ...