Labshake search
Citations for New England Biolabs :
8751 - 8800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 25 µL 2x NEBNext Master Mix (M0541, NEB)) ...
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bioRxiv - Cell Biology 2023Quote: ... we first cut pHAGE-CMV-EGFP-W1 with NotI (NEB # R0189S), followed by the treatment with DNA Polymerase I ...
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bioRxiv - Cell Biology 2023Quote: ... Then EGFP fragment was released by cutting with BamHI (NEB # R0136S), followed by separation and purification of vector fragment for ligation ...
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bioRxiv - Cell Biology 2023Quote: ... P7-EGFP-Tnrc6a was cut with AgelI (NEB # R0552S), followed by the treatment with DNA Polymerase I ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow Fragment (NEB # M0210S) to blunt the end ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed with Q5 polymerase (NEB) unless specified otherwise ...
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bioRxiv - Molecular Biology 2023Quote: Escherichia coli DH5-α (New England Biolabs) was used as the cloning and testing strain in this work ...
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bioRxiv - Plant Biology 2023Quote: ... 34uL of the modified dpGreenBarT and unmodified pGII0125 were digested with 1ul each FspI and AclI in CutSmart buffer (NEB) for 1hr at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids for this work were constructed using the Gibson-Assembly technique (NEBuilder protocol, New England Biolabs, Germany). Plasmid constructions were first established in E ...
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bioRxiv - Microbiology 2023Quote: ... GPRC5A-FLAG and GPRC5A expressing plasmids were generated by PCR of the entire coding sequence of GPRC5A from TREx BCBL1-Rta cDNA and cloning using NEBuilder HIFI DNA assembly kit (NEB) into pLENTI-CMV-GFP-PURO ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was generated using ProtoScript first strand cDNA synthesis kit (NEB, E6300S) with oligo-dT primers ...
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bioRxiv - Systems Biology 2023Quote: ... and M-MuLV Reverse Transcriptase (NEB M0253). qPCR was performed in biological and technical triplicate using an Applied Biosystems QuantStudio 3 Real-Time PCR System with Fast SYBR Green Master Mix (Thermo Fisher 4385617) ...
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bioRxiv - Synthetic Biology 2023Quote: ... were combined by Gibson Assembly (NEB E2621S) to form pBBR1_DVK_AE ...
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bioRxiv - Synthetic Biology 2023Quote: All cloning was performed in NEB 5-alpha Competent Escherichia coli (New England BioLabs C2987U). The Pseudomonas putida KT2440 strain was purchased from ATCC (#47054) ...
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bioRxiv - Synthetic Biology 2023Quote: ... An appropriate volume of reaction mastermix was prepared with the following composition: 20% v/v BsaI (New England BioLabs R3733S) or BbsI (Thermo Scientific ER1012) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains NEB Turbo and NEB Express (New England BioLabs) were used for cloning ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR product was incubated with DpnI (NEB, USA) in 1 hour at 37°C to digest the template plasmid.
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bioRxiv - Synthetic Biology 2023Quote: ... The amplified fragments were cloned into linearized pDest_pK7WG2_pRPS5A_CTP OleGFP digested by AatⅡ and PacI (New England BioLabs).
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bioRxiv - Synthetic Biology 2023Quote: Primers (Table S1) were ordered from Integrated DNA Technologies (IDT) and constructs were PCR-amplified with Q5 High Fidelity polymerase (NEB). For all vectors ...
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bioRxiv - Synthetic Biology 2023Quote: ... we performed the ligation of fragments from module plasmids in the presence of BsaI-HF (New England BioLabs, Ipswich, MA, USA) to generate array plasmids ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli Turbo (NEB) cells were used for plasmid construction ...
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bioRxiv - Synthetic Biology 2023Quote: ... These fragments were cloned into linearized p1HD digested by PvuII-HF (NEB) using an In-Fusion Cloning system ...
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bioRxiv - Synthetic Biology 2023Quote: ... The folA variable region was amplified by PCR (PhusionTM, ThermoFisher cat #F530 or Q5®, NEB cat #M0492) with 500 nM primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Q5 2x master mix (NEB) and 20 µl of sorted cell material input suspended in diluted PBS (VWR) ...
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bioRxiv - Synthetic Biology 2023Quote: ... we used the Gibson Assembly® master mix (NEB) with PCR products amplified from pErv3 and custom gene fragments (IDT gBlocks™ ...
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bioRxiv - Synthetic Biology 2023Quote: ... ER and AD variants were obtained with PCR-amplified gene fragments (IDT gBlocks™) using restrictionbased cloning with enzymes purchased from NEB. Information on tested plasmids ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain was purchased from Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 40 µl 10X phi29 DNA Polymerase Reaction Buffer (NEB) and 40 µl ddH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... HDR enhancing factors and MS2 tag were cloned between Esp3I sites by Golden Gate Assembly (New England Biolabs). All the primers are listed in Supplementary Table 2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and nickase (D10A) Cas9 mutations as well as PRDM9 catalytic mutation (G172A) were introduced by site directed mutagenesis (New England Biolabs). The collection of HDR enhancing factors fused to MS2 protein were constructed based on the pcDNA™3.1 vector backbone (Thermo Fisher) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 20 µl phi29 DNA Polymerase (10 U/µl, NEB) and incubated for 12 h at 30 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... with standard restriction enzymes (New England BioLabs; HF enzymes were used when possible) and ligation with T4 DNA ligase (New England BioLabs) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 5 µl T4 DNA ligase (both NEB) and 5 µl FastDigest Eco31I (BsaI isoschizomer from ThermoFisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and quick change PCR reactions were performed using Vent DNA polymerase (purchased from New England Biolabs, NEB) and oligonucleotide primers purchased from Eurofins Genomics ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 5 µl of the plasmid DNA served as template for a 50 µl PCR (Q5 High-Fidelity 2X Master Mix, NEB) with primers PK412+PK421 (input library ...
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bioRxiv - Synthetic Biology 2023Quote: ... and quick change PCR reactions were performed using Vent DNA polymerase (purchased from New England Biolabs, NEB) and oligonucleotide primers purchased from Eurofins Genomics ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... A repair reaction was run using the PreCR kit (NEB) as described by the manufacturer followed by SPRI bead purification and elution in 51 µl ddH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... and XhoI enzymes (NEB). Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ligation with T4 DNA ligase (New England BioLabs). All PCRs were performed with Q5 High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCRs were performed with Q5 High-Fidelity DNA Polymerase (New England BioLabs) or Phusion High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... or Phusion High-Fidelity DNA Polymerase (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Each pooled library was PCR amplified using Phusion polymerase (F-530) (New England Biolabs, M0530S). Quality of the libraries was assessed with a Bioanalyzer using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25-34 nt fragments isolated from the gel were dephosphorylated using T4 Polynucleotide kinase (New England Biolabs, M0201L). For each library sample ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... 80 U MuLV reverse transcriptase (New England Biolabs, USA) and 0.75 U poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.75 U poly(A) polymerase (New England Biolabs, USA), was subjected to 42 °C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM dNTPs (New England Biolabs, USA), 1 µM RT-primer (GGTCCAGTTTT TTTTTTTTTTTVN [V is A ...