Labshake search
Citations for New England Biolabs :
8701 - 8750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 0.2 µL of Phusion High-Fidelity DNA Polymerase (NEB #M0530), 4.5 µL of Phusion HF Buffer (NEB #B0518S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4.5 µL of 5x Phusion HF Buffer (NEB #B0518), and 1.6 µL of 2.5 mM dNTPs (NEB #N0447 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4 µL of 5x Phusion HF Buffer (NEB #B0518S), and 0.4 µL of 10 mM dNTPs ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.25 µL of 10x T4 DNA Ligation Reaction Buffer (NEB #B0202S), and 0.62 µL of 2 mg/mL BSA (NEB #B9001S ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.2 µL of Phusion DNA Polymerase (NEB #M0530S), 4 µL of 5x Phusion HF Buffer (NEB #B0518) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCRs were performed with Q5® High-Fidelity 2X Master Mix (New England Biolabs) with the designed synthetic primers (SigmaAldrich) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.4 µL of Phusion High-Fidelity DNA Polymerase (NEB #M0530S), 8 µL of 5x Phusion HF Buffer (NEB #B0518S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.62 µL of 2 mg/mL BSA (NEB #B9001S) with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2.5 µL of 10x T4 DNA Ligation Reaction Buffer (NEB #B0202S), and 0.125 µL of 60 mg/mL BSA (NEB #B9001S ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.6 µL of 2.5 mM dNTPs (NEB #N0447), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of Phusion HF Buffer (NEB #B0518S), and 0.8 µL of 10 mM dNTPs (Takara #4030) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The barcode plasmid libraries were used to transform T7 Express chemically competent cells (NEB #C2566I) to establish barcoded E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 5x Phusion HF Buffer (NEB #B0518S), and 0.8 µL of 10 mM dNTPs (Takara #4030) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and other molecular biology reagents were purchased from New England Biolabs (NEB). Plasmid extraction mini kit and Gel/PCR mini kit were purchased from Favogen Biotech Corporations.
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bioRxiv - Plant Biology 2023Quote: ... Luna® Universal qPCR Master Mix (NEB) was used for qPCR on an ABI StepOne Plus real-time system (Life Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... Restriction digest and T4 DNA Ligase (all NEB enzymes) were used to first clone the 3’-region of hmx1 downstream of a CFP and Gm resistance cassette ...
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bioRxiv - Synthetic Biology 2023Quote: ... the DNA fragments were PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs Inc) following manufacturer instructions and the PCR reactions were purified using PureLink™ PCR Purification Kit (Invitrogen).
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bioRxiv - Synthetic Biology 2023Quote: ... with DNA fragments obtained by PCR with the Q5 polymerase (NEB) [21] ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli NEB 5-a (New England Biolabs) was used for cloning ...
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bioRxiv - Synthetic Biology 2023Quote: ... Golden Gate assembly was performed using New England Biosciences (NEB) Golden Gate Assembly Kit (BsaI-HFv2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Restriction enzymes were purchased from NEB. DNA purification was performed using EZNA Cycle Pure Kits (Omega Bio-tek D6492-01 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gibson assembly was performed according to the Gibson Assembly Protocol (NEB E5510) but Gibson Assembly Master Mix was made in lab (final concentration 100 mM Tris-HCL pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... In the same tube added a 1x cut smart buffer (NEB), DNA (100 ng) ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S) was used for assigning indices to all samples prior to pooling ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μL of Round1 barcode mix (1x T4 DNA ligase buffer, 16 M/μL T4 DNA ligase (M0202L, NEB), 0.25 M/μL RNase Inhibitor ...
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bioRxiv - Biophysics 2023Quote: ... the use of a Type IIs restriction enzyme (BsaI-HF-v2, New England Biolabs) that produces unique 4 bp sticky ends ensures that the three fragments are oriented in a unique way.
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bioRxiv - Biophysics 2023Quote: ... 500 units of T4 ligase (New England Biolabs) / μg DNA / 300 μl volume were used ...
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bioRxiv - Biophysics 2023Quote: ... to incorporate a recognition site for BsaI-HF-v2 (New England Biolabs) in fragments 1 ...
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bioRxiv - Biophysics 2023Quote: ... Digestion reaction: The enzyme BsaI-HF-v2 (New England Biolabs) was used to digest the fragments at a concentration of 1.5 to 3 units / μg of DNA / 20 μl reaction volume ...
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bioRxiv - Genetics 2023Quote: ... Amplicons were PAGE purified and digested with NheI and KpnI (NEB). Library inserts were cloned into pmrPTRE-floxed-AAV with T4 ligase (Enzymatics ...
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bioRxiv - Bioengineering 2023Quote: ... the upstream and downstream digested dsDNA were ligated to each other by T4 DNA ligase (New England Biolabs). To unbind the ligase from DNA ...
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bioRxiv - Bioengineering 2023Quote: ... The upstream and downstream PCR products were digested by BssSI restriction enzyme (New England Biolabs, Ipswich, MA, USA). After another round of agarose electrophoresis and gel purification ...
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bioRxiv - Molecular Biology 2023Quote: ... and 10 µL Quick T4 DNA Ligase (NEB, M2200L). The reaction mixture was incubated for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from 10 ng of RNA using the NEBNext Ultra kit (New England BioLabs, Ipswich, MA, USA) and sequenced on an Illumina HiSeq 4000 with a 75 bp paired-end read ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested bovine serum albumin (NEB, P8108S) was analysed between each sample to avoid sample carry-over and to assure stability of the instrument and QCloud (45 ...
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bioRxiv - Molecular Biology 2023Quote: ... 7 µL Ultra II End-prep reaction buffer (NEB, E7647A), 3 µL Ultra II End-prep enzyme mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µL Ultra II End-prep enzyme mix (NEB, E76468). The mixture was incubated at 20 °C for 5 minutes and 65 °C for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
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bioRxiv - Cancer Biology 2023Quote: ... annealed into double stranded sgRNA oligos and cloned into LentiCRISPRv2 plasmid using one step BsmBI (NEB #R0580) digestion and T4 ligase (NEB #M0202S ...
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bioRxiv - Cancer Biology 2023Quote: ... digestion and T4 ligase (NEB #M0202S) reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... using Phusion High-Fidelity DNA Polymerase (NEB) and primers containing the appropriate mutations (Supplemental Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (NEB).
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bioRxiv - Microbiology 2023Quote: ... The flanking regions were amplified by Phusion High-Fidelity DNA polymerase (New England BioLabs) with the primers listed in Table S2.
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bioRxiv - Microbiology 2023Quote: ... A region containing the putative promoter region of the fhuD1/spm operon was amplified by Phusion High-Fidelity DNA polymerase (New England BioLabs) with the primers listed in Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Colony PCR reactions were performed with Taq polymerase (NEB). QiaQuick PCR purification kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... The T7 promoter and mtdTomato CDS were amplified from pmrPTRE-AAV using PTRE_floxed_F/R and Phusion High-Fidelity PCR Master Mix (NEB). NotI and SalI sites added by the primers were used to subclone this amplicon into pRM1506_TMM432 ...
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bioRxiv - Genetics 2023Quote: ... Library inserts were cloned into pmrPTRE-floxed-AAV with T4 ligase (Enzymatics) and transformed into chemically competent DH5α (NEB). Outgrowths were plated on LB agar plates with 100 µg/mL carbenicillin ...
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bioRxiv - Genetics 2023Quote: ... using 1x Quickload Taq Mastermix (NEB) with the following cycling conditions ...