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Citations for New England Biolabs :
8551 - 8600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 10 μL of 5x HF Buffer (NEB, cat. no: M0530L), 1 μL of 10 mM dNTPs (Promega ...
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bioRxiv - Immunology 2023Quote: ... and 0.5 μL of Phusion Polymerase (NEB, cat. no:M0530L). The m-alpha-RC1 and m-beta-RC1 primers hybridize to the constant region of the α-chain and β – chain genes in the mouse respectively ...
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bioRxiv - Immunology 2023Quote: ... and subsequently modified by the addition of a 7-methylguanosine cap with the Vaccinia Capping System (New England Biolabs [NEB]) using the NEB Capping protocol (NEB ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of T4 RNA ligase buffer (NEB, cat. no: M0204S), 1 μL of 10 mM ATP (NEB ...
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bioRxiv - Immunology 2023Quote: ... 1 μL of 10 mM ATP (NEB, cat. no: M0204S), 1 μL of 10 μM M13 Ligation primer ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of T4 RNA Ligase (10 000 U/mL, NEB, M0204S), and 10 μL of PEG 8000 50% (NEB ...
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bioRxiv - Immunology 2023Quote: ... 10 μL of 5x HF Buffer (NEB, cat. no: M0530L), 5 μL of SYBR Green (1:50) ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... and then ligated by T4 DNA ligase (NEB). After reversing cross-links ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Genetics 2023Quote: ... 5 units of Antarctic Phosphatase (NEB) and 10 mU/µL of phosphodiesterase I (PDEI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mutated enhancer sequence was synthesized as a gBlocks Gene Fragment (IDT, Coralville IA) and inserted into pattBnucGFPh using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Beverley, MA).
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bioRxiv - Evolutionary Biology 2023Quote: ... Each of the synthesized DNA fragments was amplified by PCR and inserted at the 5’ of the GFP gene in the pPha-NR vector (NovoPro Bioscience) using NEBuilder HiFi DNA Assembly (New England BioLabs). Five μg of the pPha-NR constructs were introduced into P ...
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bioRxiv - Genetics 2023Quote: ... Nicks in the DNA were repaired with PreCR Repair Mix (New England Biolabs). Samples were barcoded with the NEBNext Ultra II DNA Library Prep Kit ...
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bioRxiv - Physiology 2023Quote: ... coli (C2987H, NEB) by heat shock at exactly 42°C for exactly 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... coli (High Efficiency) (New England Biolabs C2984H) and purified using the NucleoBond Xtra Maxi EF kit (Machery Nagel 740424).
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bioRxiv - Molecular Biology 2023Quote: ... NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer’s instructions for end repair ...
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bioRxiv - Molecular Biology 2023Quote: ... The deaminated ΦX174 DNA was purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 ng of deaminated DNAs were digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich, MA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 - 400 ng of PCR fragments were used as template DNA to synthesize analytic amounts of DNA deaminases using PURExpress In Vitro Protein Synthesis kit (NEB, Ipswich, MA) following manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5µM of NEBNext Unique Dual Index Primers and 25 µL NEBNext Q5U Master Mix (New England Biolabs, Ipswich, MA, USA) were added to the DNA and PCR amplified ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The custom made Pyrollo-dC adaptor (NEB Organic Synthesis Division, Ipswich MA), where all dCs are replaced with Pyrollo-dC ...
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bioRxiv - Molecular Biology 2023Quote: ... digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich MA) and the reaction products were quantified with LC-MS/MS.
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bioRxiv - Molecular Biology 2023Quote: ... deaminase treated RNA was purified with NEBNext sample purification beads (E7104S, NEB, Ipswich, MA) using 1.2x beads to sample volume ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) was added and incubated additional 30 min at 37°C followed by 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μL of PURExpress sample was mixed with 300 ng of ΦX174 Virion DNA (ssDNA substrate, NEB Ipswich MA) or ΦX174 RF I DNA (dsDNA substrate ...
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bioRxiv - Molecular Biology 2023Quote: ... or ΦX174 RF I DNA (dsDNA substrate, NEB, Ipswich MA) into buffer containing 50 mM Bis-Tris pH 6.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Template DNA for in vitro protein synthesis was generated with Phusion® Hot Start Flex DNA Polymerase (NEB. Ipswich MA) using gBlocks as template and flanking primers (Supplementary Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting Fluc mRNA was purified twice by Monarch RNA cleanup kit (T2040S, NEB, Ipwsich, MA) to ensure that unincorporated nucleotides were completely removed ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified and filtrated RNA was then digested to single nucleoside by Nucleoside Digestion Mix (M0649S, NEB, Ipswich, MA). LC-MS/MS analysis was performed by injecting digested stable isotope labeled RNAs on an Agilent 1290 Infinity II UHPLC equipped with a G7117A diode array detector and a 6495C triple quadrupole mass detector operating in the positive electrospray ionization mode (+ESI) ...
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bioRxiv - Plant Biology 2023Quote: ... Chemical fragmentation of ligated RNA to ≤200nt was performed using the Magnesium RNA fragmentation kit (New England Biolabs, cat#E6150S). 2ul RNA Fragmentation Buffer was added and samples were incubated at 94°C for 5 minutes followed by a transfer to ice and the addition of 2μl of RNA Stop solution ...
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bioRxiv - Plant Biology 2023Quote: ... and 2μl random primer mix (New England Biolabs, cat#S1330S) were mixed and incubated for 10 minutes at 23°C then put on ice for 1 minute ...
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bioRxiv - Plant Biology 2023Quote: ... 1X T4 RNA ligase 1 buffer (New England Biolabs, cat#B0216L), 25% PEG8000 with 1μl (40U ...
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bioRxiv - Plant Biology 2023Quote: ... Second-strand synthesis was performed using 5U Klenow fragment (New England Biolabs, cat#M0210S) with 100uM dNTPs and 1μM of iPARE adapter primer (5’-NNNNTCTAGAATGCATGGGCCCTCCAAG-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were carried out using the Q5 DNA polymerase (NEB) on Arabidopsis cDNA libraries or genomic DNA ...
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bioRxiv - Plant Biology 2023Quote: ... and 1μl T4 RNA ligase 1 (New England Biolabs, cat#M0204S) in a reaction volume of 100μl ...
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bioRxiv - Cell Biology 2023Quote: ... and dNTPs (NEB N0447L) to create complementary DNA (cDNA) ...
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bioRxiv - Synthetic Biology 2023Quote: ... then RNA was purified using a Monarch® RNA Cleanup Kit (NEB T2030). For in vivo samples ...
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bioRxiv - Synthetic Biology 2023Quote: ... then directly treated with DNAse I-XT (NEB M0570) at 37°C for 30 minutes to remove DNA template ...
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bioRxiv - Biochemistry 2023Quote: ... The sgRNA cassettes were then ligated in place of the filler using T4 DNA ligase (NEB).
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bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina Standard library construction (New England Biolabs) and sequencing (Illumina NovaSeq ...
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bioRxiv - Biochemistry 2023Quote: ... via Gibson Assembly Master Mix (NEB, Cat # E2611S) where both dSaCas9 and its targeting-gRNA (targeting Chr 9 ...
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bioRxiv - Biochemistry 2023Quote: ... via T4 DNA ligase (NEB, Cat # M0202S). After generation of this AID-C12*-nCas9-BE4max construct ...
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bioRxiv - Bioengineering 2023Quote: ... Frozen cell pellets were resuspended in 4 mL of IMAC buffer (NEB, Ipswich, MA, USA) on ice and dispersed using an ultrasonic disruptor (Sonics ...
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bioRxiv - Plant Biology 2023Quote: ... PolyA mRNA was enriched using the protocol of NEBNext ® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs #7490 S, USA). RNA sequencing libraries were then prepared using Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit (Yeasen ...