Labshake search
Citations for New England Biolabs :
8501 - 8550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... and PCR amplification with Q5® High-Fidelity 2X Master Mix (NEB, catalog no. M0492S). PCR products were confirmed by Sanger sequencing (Genewiz)
-
bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed using Q5 polymerase (NEB, M0491). The final indexed and purified DNA was spiked-in for additional sequencing of 5,000 reads per cell.
-
bioRxiv - Genomics 2023Quote: ... The reaction was diluted to 100 µL with 1X T4 PNK (polynucleotide kinase) buffer and incubated for 30 min with 1 µL T4 PNK (10 U/µL; NEB M0201) to remove 3’ phosphoryl groups ...
-
bioRxiv - Genomics 2023Quote: ... Dephosphorylation was performed by adding 10X Antarctic phosphatase buffer and 0.5 µL of Antarctic Phosphatase (NEB M0289S) prior to incubation for 2 h at 37 °C ...
-
bioRxiv - Genomics 2023Quote: ... and then prepared into a whole-genome shotgun library using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA). Instead of the standard Illumina library prep adaptor ...
-
bioRxiv - Genomics 2023Quote: ... a dual-enSERT-2.1 plasmid (1 μg) was digested overnight with PauI (NEB, R0199) at 50°C in rCutSmart Buffer ...
-
bioRxiv - Genomics 2023Quote: ... Genomic DNA was digested by using high-fidelity SbfI (New England Biolabs) and the resulting fragments were double barcoded ...
-
bioRxiv - Genomics 2023Quote: ... +28 μl M.EcoGII at 5 U/μl (NEB M0603S). The DNA was purified using Qiagen PCR columns and quantified by measuring A260 ...
-
bioRxiv - Genomics 2023Quote: ... (2) +28 μl Dam at 8 U/μl (NEB M0222L); (3 ...
-
bioRxiv - Genomics 2023Quote: ... the plasmids were digested with BamHI-HF (NEB, R3136L) and EcoRV-HF (NEB ...
-
bioRxiv - Genomics 2023Quote: ... 0.3 μg DNA was digested with 2 U DpnI (NEB R0176S) or 5 U MboI (NEB R0147S ...
-
bioRxiv - Genomics 2023Quote: Each library was converted to cDNA with Induro RT (NEB M0681S) using a 57-mer ssDNA as a primer ...
-
bioRxiv - Microbiology 2023Quote: ... and DNA assembly was achieved with the NEBuilder HiFi mix (New England Biolabs). The B ...
-
bioRxiv - Genomics 2023Quote: ... The product was purified with 1.8× Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by addition of 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
-
bioRxiv - Genomics 2023Quote: ... 0.3 μL oxidation supplement (NEB), 0.3 μL oxidation enhancer (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... 1.5 μL NEBuffer 4 (NEB), 0.75 μL T4 Phage β-glucosyltransferase (NEB M0357S) ...
-
bioRxiv - Genomics 2023Quote: ... The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB) and unmethylated lambda DNA (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... and unmethylated lambda DNA (NEB). CpG methylated pUC19 DNA and unmethylated lambda DNA were used to measure conversion rate of 5mC and unmodified C.
-
bioRxiv - Genomics 2023Quote: ... 0.16 μL Q5 (NEB M0491S), 0.12 μL 120ng/μL sonicated carrier lambda DNA ...
-
bioRxiv - Genomics 2023Quote: ... 0.75 μL T4 Phage β-glucosyltransferase (NEB M0357S), and 12.45 μL water ...
-
bioRxiv - Genomics 2023Quote: ... 0.3 μL oxidation enhancer (NEB), 0.3 μL DTT (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... BGT reaction was terminated by addition of 0.75 μL Proteinase K (NEB P8107S) and incubation at 37°C for 30 min ...
-
bioRxiv - Microbiology 2023Quote: ... and were cloned by Gibson Assembly55 using Gibson Assembly Mastermix (NEB) to produce an infectious ...
-
bioRxiv - Genomics 2023Quote: ... the oxidized DNA was purified with 1.8X Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by adding 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR was performed in a 25 μl volume with 0.08 μM primer concentration and NEBNext Q5 Hot Start HiFi PCR Master Mix (ref. M0543L, New England Biolabs). The cycling conditions were an initial denaturation of 30 s at 98°C followed by 5 cycles of 98°C for 10 s ...
-
bioRxiv - Microbiology 2023Quote: ... followed by purification using the Monarch Gel Extraction Kit (New England Biolabs, catalog no. T1020L).
-
bioRxiv - Microbiology 2023Quote: RNA extracts from WWI samples were reverse transcribed to synthesize cDNA using the LunaScript SuperMix RT kit (New England Biolabs, E3010L); 10 µL of each RNA extract was used in a total reaction volume of 20 µL ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was diluted 1:10 and 3 µl of diluted cDNA was used as input for qPCR using Luna by NEB master mix for a 20 µL total reaction ...
-
bioRxiv - Genomics 2023Quote: ... except that the In-Fusion HD reaction was replaced by Gibson assembly® (New England BioLabs, #E2611S). Briefly ...
-
bioRxiv - Genomics 2023Quote: ... Total (“steady-state”) RNA was converted to sequencing libraries using NEB polyA capture kit (product no) followed by NEB Ultra directional II RNA-seq library kits ...
-
bioRxiv - Genomics 2023Quote: ... followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 (NEB# E6440) ...
-
bioRxiv - Genomics 2023Quote: ... Libraries were prepared from purified transposed DNA using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) and Illumina indexing primers ...
-
bioRxiv - Genomics 2023Quote: ... Resulting sequences were cloned upstream of the core promoter (using KpnI restriction site) via Gibson assembly® (New England BioLabs, #E2611S). Each construct was verified using sanger sequencing and luciferase assays were performed as previously described1 ...
-
bioRxiv - Genomics 2023Quote: ... with the ligation of 3′-small RNA Tru-Seq adapter using the truncated T4 RNA Ligase 2 (NEB). 45-150 nt capped small RNAs were recovered on 15% Urea-TBE gel (Novex ...
-
bioRxiv - Genomics 2023Quote: ... Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), custom Nextera PCR primers as previously described (29) ...
-
bioRxiv - Genomics 2023Quote: ... The number of library amplification cycles was determined by qPCR analysis using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) on LightCycler® 96 System (Roche) ...
-
bioRxiv - Genomics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 (NEB# E6440). The generated DNA libraries were purified using Agencourt® AMPure® XP (Beckman Coulter) ...
-
bioRxiv - Genomics 2023Quote: ... Library preparation has been done with 250ng of input RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
-
bioRxiv - Genomics 2023Quote: ... PCR amplification was performed for 7-11 cycles (depending on input DNA concentration) using NEBNext Mulitplex Oligos (New England Biolabs). Indexed sample concentration was quantified using the KAPA Library Quantification Complete Kit (Universal)(Roche) ...
-
bioRxiv - Genomics 2023Quote: ... NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) was used to prepare indexed sequencing libraries following the manufacturer supplied protocol ...
-
Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... and Monarch® RNA purification kit (NEB) was used to purify the final mRNA according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2023Quote: ... ligated with the pLV backbone using T4 DNA ligase and the NEB Stable Competent Escherichia coli host cells (#C3040H, New England Biolabs, MA, USA) were transformed ...
-
bioRxiv - Genetics 2023Quote: ... The library preparation was done using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced on the NextSeq500 instrument (Illumina) ...
-
bioRxiv - Immunology 2023Quote: ... The library was diluted to 50 ng/uL in water and electroporated to competent (NEB DH5a cells) cells with an efficiency of ≥109 cfu/ug ...
-
bioRxiv - Cancer Biology 2023Quote: ... An RNA-seq library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... 0.5 μL Cas12a (100 μM) (NEB) and 0.2 μl of 10X NEB 2.1 buffer were mixed gently in a PCR tube and incubated at 25°C for 15 minutes in a Thermal Cycler (Bio-Rad T100 ...
-
bioRxiv - Genetics 2023Quote: ... double-stranded Lambda DNA probe (New England Biolabs, N3011S). Numerical values were assigned to the agarose-gel readings by measuring selected individuals using the Qubit 2.0 Fluorometer.
-
bioRxiv - Genetics 2023Quote: ... cDNA was then used as a template for qPCR using the Luna Universal Probe Master Mix (New England Biolabs, Ipswich, Massachusetts) with the Universal Probe Library #21 probe (Roche ...
-
bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs]) per well using a FACSAriaIII instrument (BD) ...
-
bioRxiv - Immunology 2023Quote: ... and 0.5 μL of Phusion Polymerase (NEB, cat. no: M0530L). PCR-2 was run using the following conditions ...