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Citations for New England Biolabs :
801 - 850 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... after which 2 µL of Recombinant PNGaseF (Glycerol-free) (New England Biolabs, Ipswich, MA; catalog # P0709L) was added to each sample ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was washed with 80% ethanol and resuspended in 12 μl nuclease free water (NEB). 1 μl of the prepared cDNA library was subjected to Bioanalyzer analysis.
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bioRxiv - Bioengineering 2024Quote: ... The genes were diluted to 125 pM in 30 µL PURExpress cell-free expression mix (NEB) containing 0.06 % pluronic acid (F-127 ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA was treated with RNase-free DNase I (New England Biolabs [NEB], Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA was treated with RNase-free DNase I (New England Biolabs [NEB], Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µg RNA was used for each reverse transcriptase reaction using M-MuLV Reverse Transcriptase (NEB) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... 10 µg of total RNAs were subjected to RNA purification using DNase I (New England Biolabs Inc.) and the quality was checked on 2% agarose gel and verified using the Agilent Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... Fragmentation of 1 µg mRNA aliquots for 5 min at 94°C in 1x Fragmentation Buffer (NEB) was optimal to obtain a pool of 100-200 nt fragments ...
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bioRxiv - Genomics 2021Quote: The GCG1/SUT098 FPR plasmid was digested with AvrII and BglII (1 U per µg plasmid, NEB) restriction enzymes to excise the NatMX6 cassette ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Total RNA (1 µg) was reverse transcribed using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S) with primer UP0118 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 µg of purified recombinant WRN protein was treated with Lambda Protein Phosphatase (LPP, New England Biolabs) in 1X PMP buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each pFRT-TO-CLIP-UPF1 mutant vector was digested with 1 µL SpeI (NEB) and 1 µL HindIII-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of DNA was mixed with 7 µl NEBNext Ultra II end prep reaction buffer (NEB) and 3 µl Ultra II end prep enzyme mix (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... RNA (1 µg per sample) was transcribed to cDNA by the Protoscript®II reverse transcriptase (NEB). clpP ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... cDNA were synthesised from 1 µg RNA by reverse transcription using LunaScript RT SuperMix Kit (NEB; E3010). qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 µg of total RNA using the LunaScript RT SuperMix kit (NEB, UK) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... then combined and treated with 1% SDS and 20 µg of proteinase K (New England Biolabs, #P8107S). The reaction was incubated for 1 h at 55°C ...
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bioRxiv - Neuroscience 2024Quote: ... tube 2: 20 µL VLP suspension + 6 µL water + 1 µL Proteinase K (200 µg/mL, NEB), and tube 3 ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of RNAs were polyadenylated by incubating with E-coli poly(A) Polymerase (PAP) (NEB M0276) at 37°C for 15 min and purified with AMPure RNA Clean XP beads ...
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bioRxiv - Microbiology 2024Quote: ... A polyC tail was added to 1 µg of end repaired DNA using Terminal Deoxynucleotidyl Transferase (NEB) with a mixture of 95% dCTP and 5% ddCTP as a substrate ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA sample (with RIN ≥ 7) was enriched for mRNA (New England Biolabs, #E7490) for each condition ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNAs were synthesized from 2 µg total RNA with Superscript II reverse transcriptase (New England Biolabs). RT-qPCR was performed in a StepOnePlus™ Real-Time PCR System (Applied BioSystems ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 40 µg of pSC5 was digested with 18 µl of SacI-HF (New England Biolabs, R3156) at 37 °C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... Purified genomic DNA (1.2 - 1.5 µg) was digested with 10 units of DpnI (New England Biolabs (NEB) R0176L ...
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bioRxiv - Genomics 2024Quote: ... Purified genomic DNA (1.2 - 1.5 µg) was digested with 10 units of DpnI (New England Biolabs (NEB) R0176L ...
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 10 µg was digested using 3 µl of EcoRI-HF (New England Biolabs, catalog number: R3101) at 37 °C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The crude circRNA product was on a silica based-column (Monarch RNA cleanup kit, 50 µg, NEB) followed by RP-HPLC ...
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bioRxiv - Systems Biology 2023Quote: ... Purified DNA fragments were assembled by One Step Cloning Kit (Vazyme Co., Ltd. Cat. C113) or Golden gate assembly54(NEB, Cat. M0202 and R3733). Sequence contents of genetic parts are collected in Supplementary Table 11.
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bioRxiv - Microbiology 2022Quote: ... 2 mM 3’-O-Me-m 7G(5’)ppp(5’)G cap structure analog (NEB S1411S), 0.5 mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Preclearing step: ∼ 50 μL magnetic beads (Protein A or G Magnetic Beads; #S1425S or #S1430S NEB) were added to the sample and incubation was carried out for 1 h on a rocking platform at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... we digested a lentiviral plasmid that contains mCherry in the location where the G viral entry protein is cloned (4016_V5LP_pHrU3_ForInd-Extgag_mcherry) with XbaI and MluI-HF (NEB, Cat ...
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bioRxiv - Cell Biology 2024Quote: ... with a combination of neuraminidase and O-Glycosidase (NA and O-G, New England Biolabs, P0733), with Peptide-N-glycosidase F (PNGase F ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sheared extracts were adapter-ligated and enriched using the standard NEBNext or NEBNext Ultra protocol (catalog #E6040 and #E7370) and NEBNext multiplex Illumina primers (catalog #E7335) (NEB, Ipswich, MA, USA) in ½-size recommended reaction volumes for end-preparation ...
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bioRxiv - Genetics 2020Quote: ... 17-25 nt small RNA fractions were isolated by denaturating PAGE and subjected to standard small RNA library preparation using the NEB Next small RNA sequencing Kit (NEB, Frankfurt a.M., Germany). The procedure includes 3′-OH and 5′-monophosphate specific ligation steps and we tried to lower 3′-2′-O-me biases by 18 hours 3′-ligation at 16°C ...
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bioRxiv - Cell Biology 2022Quote: ... and protein samples and molecular weight markers (PageRuler Plus Pre-stained Protein Ladder, ThermoFisher Scientific 26619, or Colour pre-stained protein standards NEB P7712 or P7719) were separated using 8% ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colony PCRs for crRNA integrations into pCRISPR-Cas3 were performed using OneTaq® 2X Master Mix with Standard Buffer Enhancer (New England BioLabs Inc., U.S.A).
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bioRxiv - Cell Biology 2023Quote: ... 10 % SDS-PAGE gel was prepared and 50 μg of protein lysate was loaded along with the protein standard ladder (Cat.No.: P7719S, New England Biolabs, Ipswich, Massachusetts, United States), and electrophoresis was performed for 1.5 hr in ice-cold 1x Laemmli electrophoresis running buffer ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum in placenta samples was evaluated using One Taq® Quick-Load® 2X Master Mix with Standard Buffer (NEB, Cat No. M0486L) and the following thermocycler program ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The total RNAs were treated with RNase-Free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) to exclude remaining genomic DNA and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted RNA was treated with 2% RNase-free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation.
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bioRxiv - Genetics 2020Quote: ... Sense oligos diluted to 10 μM in nuclease-free water were phosphorylated with T4 PNK (NEB #M0201) supplemented with ATP ...
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bioRxiv - Genetics 2020Quote: ... and the residual DNA was removed with RNase-free DNase I (New England BioLabs, Ipswich, MA, USA) for 30 min at 37 °C ...