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Citations for New England Biolabs :
651 - 700 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... overhang sites were restriction enzyme digested and then assembled into Tweety-integrating zeocin marked MOP plasmids using the Gibson Assembly standard protocol (E5510, New England Biolabs). Successful plasmid construction was verified using whole plasmid sequencing with Oxford Nanopore Technologies (ONT ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’UTR fragments were then cloned into restriction sites (NheI and BamHI) downstream of Cerulean in the pFmiR-empty backbone (16) using standard ligation protocols (NEB). Plasmids were verified by Sanger sequencing (Molecular Biology Services Unit ...
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bioRxiv - Microbiology 2024Quote: ... the homology arms of the target genes were amplified from the genomic DNA (gDNA) of Ab-C98 using Taq DNA Polymerase with Standard Taq Buffer (NEB), and the apmR was amplified from the plasmid pCasAb-apr [37] ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was converted to cDNA by standard reverse transcription with M-MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA) and oligo(dT ...
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bioRxiv - Microbiology 2024Quote: ... was mixed with an 80-mer spike-in RNA oligonucleotide internal standard (DNA and RNA oligonucleotide sequences in Table S5).5 The mixture was dephosphorylated shrimp alkaline phosphatase (rSAP, NEB) in NEB T4 RNA ligase buffer at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Precipitated cDNAs were stored at -20°C before performing PCR using P5/P7 standard Illumina primers and Phusion HF master mix (NE-M0531L, NEB). Ribosomal RNA contaminants were removed from the final library using Ribocutter which utilises Cas9-guided rRNA depletion (Wilkins and Ule ...
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bioRxiv - Synthetic Biology 2024Quote: ... the appropriate linearized plasmid and purified DNA block was mixed into the NEBuilder HiFi DNA assembly master mix following standard protocol (NEB) and then transformed into dSL2 donor cells ...
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bioRxiv - Genetics 2024Quote: ... Proceeded to DNA library prep using 25ng of input and following the standard protocol for the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). During the PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... All plasmids were constructed using standard cloning methods with New England Biolabs restriction enzymes and T4 DNA ligase (New England Biolabs) or with the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs of cDNA derived from Bottlenose dolphin tissues were performed with the One Taq 2X Master Mix with Standard Buffer (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Decapping of 5 µg total RNA was performed with 200 units of yDcpS (NEB M0463S) in 10mM Bis-Tris-HCl pH 6.5 ...
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bioRxiv - Biophysics 2021Quote: ... 15 µg of the purified nicked minicircle was incubated with T4 ligase (New England Biolabs) and ligase buffer containing 25 µg mL-1 BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA), 0.2 μM of each primer and 2 μl of template DNA ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and DNase was removed using the Monarch RNA Cleanup Kit (50 µg; New England Biolabs). LunaScript RT SuperMix Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µg of pRS193 lentiviral cloning backbone plasmid was digested by EcoRI-HF (NEB #R3101S) and XbaI (NEB #R0145 ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA (2 µg) was first annealed with 200 ng random nonamer primers (NEB S1254S) by incubation at 70°C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed on 0.5 µg DNase-treated total RNA using Lunascript RT (NEB) in 20µl reactions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Plant Biology 2023Quote: ... 100 µg of dual crosslinked enriched chloroplasts were resuspended in 1X DpnII reaction buffer (NEB) and digested with 20 U DpnII at 37°C overnight with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM EDTA] plus 1 µL 20 µg/µL lambda phage DNA (NEB, cat # N3011S) and was separated on a 6% polyacrylamide / 0.5x TBE / 1.5 mm gel for 2 hr at 110 V in a Novex electrophoresis system ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µg of this plasmid along with 50 units of MfeI restriction enzyme (NEB R3589), which cuts at a single site within the PfHO gene just upstream of the homologous sequence cloned into the donor-repair pGDB plasmid ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µg of RNA was used to synthesize cDNA by oligo dT (New England Biolabs) and reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µg of total RNA was used with D(T)23VN primer (New England Biolabs), 10 mM dNTPs (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
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bioRxiv - Synthetic Biology 2024Quote: ... was coated with 25 µL/well of 2 µg/µL streptavidin (New England Biolabs; N7021S) diluted in 1x HBS and incubated overnight at 4 °C ...
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bioRxiv - Systems Biology 2024Quote: ... and VPS4B_KO cell lines.200 µg of proteins were digested with LysC (New England Biolabs) by adding 0.4 µg LysC (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of the vector backbone (pSP0769) was linearized using PmeI (NEB, Cat. No. R0560S) for 1h and gel purified.
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Biochemistry 2023Quote: ... and then the two PCR products were co-transformed into yeast along with pCB05 digested with BstXI (New England Biolabs [NEB], Catalog # R0113S) and XhoI (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... in the presence of m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB). 5 μg DENV-Luc RNA was electroporated into 2×106 Vero cells ...
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bioRxiv - Genetics 2022Quote: ... The m7G(5’)ppp(5’)G RNA Cap (New England BioLabs, catalog number S1404L) was used as Cap Analog with 4:1 of Cap Analog:GTP ratio ...
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bioRxiv - Evolutionary Biology 2020Quote: ... treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs, USA), then used 1 μL treated RNA in cDNA synthesis with SuperScript III Reverse Transciptase (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... The total RNA was then treated with RNase-free DNase I (New England BioLabs Inc.), and column purified using ISOLATE II RNA Mini Kit (Bioline ...
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bioRxiv - Genetics 2020Quote: Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase ...
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bioRxiv - Plant Biology 2021Quote: The extracted RNA was treated with RNase-free DNase (New England Biolabs, Ipswich, MA, USA). Quality control measurements were performed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Plant Biology 2021Quote: ... the extracted RNA was treated with RNase-free DNase I (New England Biolabs, https://www.neb.com) for 30 min at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... the extracted RNA was treated with RNase-free DNase I (New England Biolabs, https://www.neb.com) for 30 min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... 2.5 μL of Taq Buffer B (Mg-free; 10X) (New England Biolabs, Ipswich, MA, USA), 2.5 μL of dNTPs (2.5 mM of each base) ...
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bioRxiv - Genetics 2022Quote: ... Then the reaction was diluted to 50µL and 2µL RNase-free DNase I (NEB, M0303S) were added ...
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bioRxiv - Plant Biology 2022Quote: ... 5 pmol of label-free RNA substrates were dephosphorylated with Alkaline Phosphatase (New England Biolabs) and labeled with [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from DNA-free RNA using random nonamers (New England BioLabs, Ipswich, MA) and SuperScript III RT (Invitrogen/ThermoFisher ...