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Citations for New England Biolabs :
701 - 750 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... polymerase along with template was used for riboprobe synthesis and RNase free DNase (NEB M0303S) was used for template digestion ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was treated with RNase-free DNase I (New England Biolabs, Ipswich, MA, USA) to remove any genomic DNA contamination ...
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bioRxiv - Genetics 2024Quote: ... Contaminating genomic DNA was removed using an RNase-free DNase I (NEB, Ipswich, MA, USA) treatment and further controlled by RT-PCR using an intron-containing CcSOD gene.
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bioRxiv - Synthetic Biology 2021Quote: ... The second plasmid is edited from the above mentioned pUC19-PCN library by inserting a single competitor via standard Site-Directed Mutagenesis(NEB, E0554). Two plasmids were co-transformed into GL002 strain that has dFnCas12a and TetR integrated in its genome with Mix & Go! E.coli Transformation Kit(T3001) ...
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bioRxiv - Synthetic Biology 2021Quote: ... NEB 10x Standard Taq Reaction buffer (100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl2, pH 8.3) (New England Biolabs, Ipswich, MA), 2× PCR reaction mix (2× Standard Taq Reaction buffer ...
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bioRxiv - Genetics 2021Quote: ... Plasmid was isolated from the remainder of the original 5-mL culture of the R599A culture using standard methods and digested with PvuI-HF (New England Biolabs #R3151) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Genomics 2020Quote: ... The resulting fragment is then ligated into a digested pCDNA3-YFP Basic plasmid that had been cut with Kpn1 and Xho1 using a standard T4 DNA Ligase protocol (NEB #M0202). Once the insert ...
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bioRxiv - Cell Biology 2021Quote: The recovery probe was prepared as follows: A standard 100 µl PCR reaction was prepared containing 10 ng of bacteriophage λ DNA (NEB), 5 units of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse (ccagtgagcttcccgttca) primers with Q5 High fidelity DNA polymerase according to the standard protocol described by the manufacturer (New England BioLabs®). The PCR products obtained after 35 cycles were separated through a 1% agarose gel ...
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bioRxiv - Biochemistry 2021Quote: ... Quantification of libraries prior to sequencing used qPCR with primers specific to the Illumina P5 and P7 adaptor sequences and standards from the NEBNext Library Quant Kit (NEB, E7630S). Sequencing of prepared libraries was performed using an Illumina MiniSeq with the 75-cycle high-output kit ...
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bioRxiv - Microbiology 2021Quote: All cDNA was made by following the “First Strand cDNA Synthesis” standard protocol provided by New England Biolabs with their AMV Reverse Transcriptase (New England Biolabs, M0277L). Random hexamers (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... MmnI in this paper) as candidate genes by searching against a gold-standard dataset in the Restriction Enzyme Database (NEB REBASE) (21) ...
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bioRxiv - Developmental Biology 2021Quote: ... The template for probe synthesis was generated through two rounds of standard PCR method using OneTaq® 2x Master Mix (New England Biolabs, NEB): the first PCRs from cDNA used the gene-specific primers including the T7 linker sequence ...
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bioRxiv - Microbiology 2021Quote: ... Resolved strains (GFP-negative and kanamycin-sensitive) were tested for the desired genotype by colony PCR (OneTaq® 2X Master Mix with Standard Buffer, New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... and BGH reverse (5’-TAG AAG GCA CAG TCG AGG -3’) primers from the pcDNA3.1+/-C-(K)-D vector using standard methods (NEB 2x Q5) which include 5-10 ng DNA per reaction ...
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bioRxiv - Synthetic Biology 2021Quote: ... Standard PCR-based amplification to add overlap-adaptors and Gibson-style assembly methods (HiFi Assembly Kit, New England Biolabs, Ipswich, Massachusetts) were used to assemble the final vectors ...
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bioRxiv - Synthetic Biology 2021Quote: minD,minE and FtsA genes were amplified by standard polymerase chain reaction (PCR) amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, USA) as previously reported24,30 ...
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bioRxiv - Microbiology 2023Quote: ... Resolved strains (GFP-negative and kanamycin-sensitive) were tested for the desired genotype by colony PCR (OneTaq® 2X Master Mix with Standard Buffer, New England Biolabs). All the plasmids are listed in Table 2.
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µL of the supernatant was used as a template for the PCR reaction using OneTaq® 2X Master Mix with Standard Buffer (New England Biolabs) primers 4925 and 4926.
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Cell Biology 2023Quote: ... New plasmids used in this study were generated using standard restriction-ligation or by using NEBuilder HiFi DNA Assembly (New England Biolabs E552OS). HsPIP5K1A ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was then used for PCR with the OneTaq 2x Master mix with Standard Buffer (New England Biolabs, Ipswich, Massachusetts). Targets for differential editing of the mutant in p150 and p110 were ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was carried out with a standard oligo-dT primer protocol using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560). RNA concentrations were normalized between samples prior to reverse transcription ...
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bioRxiv - Genetics 2022Quote: ... along with 6uL of EZ-Run Pre-Stained Rec Protein Ladder (Fisher BP3603-500) and 3uL of Blue Prestained Protein Standard (NEB P7718S). Gels were electrophoresed in XT Tricine Running Buffer (Bio-Rad 1610790 ...
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bioRxiv - Microbiology 2023Quote: ... using a minimum of 20 bp overlapping regions between DNA fragments with custom made kit Plasmid selection and verification after the transformation were checked using the OneTaq® 2X Master Mix with Standard Buffer (NEB). Plasmids were purified using the Qiagen plasmid purification kit according to manufacturer’s protocol.
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bioRxiv - Genetics 2024Quote: Total RNA from mouse glomeruli was sequenced on a HiSeq 4000 following standard workflow using NEBNext Ultra II RNA Library Preparation kit (New Englab Biolabs, MA)(Illumina ...
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bioRxiv - Immunology 2024Quote: ... the synthesis of biotin labeled probes was conducted by PCR in a 50 µl reaction volume with one Taq® standard reaction buffer (Biolabs), 0.2 mM dNTPs where dCTP ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by genotyping PCR and gel electrophoresis using the OneTaq® 2X Master Mix with Standard Buffer (NEB, Catalog #M0482S) following manufacturer’s instructions for reaction set-up and the oligonucleotides and thermocycler conditions described in Supplemental File 3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Vector backbone was amplified to introduce BsmbI sites using primers (AACGTCTCatggtttagtgaaccgtcagatcgaattc, AACGTCTCagctctgcttatatagacctcccacc) and Phusion polymerase in GC buffer using standard manufactures protocol (NEB, M0530). The purified amplicon was ligated to annealed oligonucleotides at a 1:7 ratio using T4 ligase and transformed into DH5α chemically competent E ...
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bioRxiv - Genetics 2021Quote: ... 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L) and NlaIII (NEB #R0125L ...
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bioRxiv - Genomics 2021Quote: ... Total RNA from A549 cells (3 µg in 30 µl) was treated with RppH (NEB M0356) at 30 °C for 1 hr and purified by spin column ...
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bioRxiv - Developmental Biology 2021Quote: ... 6-8 µg of Cas9-mSA plasmid was linearized by Not1-HF restriction enzyme (NEB #R3189S). The 8.8kb linearized fragment was cut out from a 1.5% agarose gel and DNA was extracted using QIAquick Gel Extraction Kit (Qiagen #28115) ...
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bioRxiv - Biochemistry 2021Quote: ... RCA products were debranched using 10 units/ µg DNA of T7 Endonuclease I (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Desalted peptides were dissolved at a concentration of 3 µg/µL in 1x CutSmart buffer (NEB, 50 mM potassium acetate ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Microbiology 2021Quote: ... For reverse transcription (RT) 1 µg RNA was digested with 2 U of DNase I (NEB). After heat inactivation of the DNase at 70 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... Then CaCl2 was added to 2mM and 1 µL (about 1 µg) of Factor Xa (NEB) was added per 50 µg of protein and the samples were left at RT for 20-24h ...
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bioRxiv - Developmental Biology 2022Quote: ... Ribosome footprints were generated by incubating the lysate with 3 U/µg of micrococcal nuclease (NEB) for 40 min at 25° C ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 16 µg genomic DNA was digested with HindIII restriction endonuclease (NEB Biolabs, UAS). Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 16 µg genomic DNA was digested with HindIII restriction endonuclease (NEB Biolabs, UAS). Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µg of the PCR product was subjected to Fragmentase® treatment (New England Biolab, NEB) until a smear of fragments was detected around 400-500pb by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 µg of the Cas9-treated vDNA was digested with either HindIII-HF or XhoI (NEB) for 3 h before being resolved on a large 0.6% agarose gel alongside an untreated control ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Biochemistry 2023Quote: The PCR product was purified and 1 µg was phosphorylated using 10 units T4 PNK (NEB) in 20 µl of T4 ligase buffer (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Genomics 2023Quote: 1-2 µg of extracted total RNA and polysomal RNA was treated with DNaseI (NEB, M0303) in a 100 µL reaction at 37 °C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI ...