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Citations for New England Biolabs :
851 - 900 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... was used to purify synthesized gRNA after DNase I (RNase-free) treatment (New England BioLabs, catalog# M0303S). 5 μg purified LbCas12a (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted RNA was treated with 2% RNase-free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... A fraction of total RNA (~20 μg) was treated with DNase I (RNase-free; New England Biolabs) at 37°C for 15 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The total RNAs were treated with RNase-Free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) to exclude remaining genomic DNA and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Molecular Biology 2021Quote: RNA used for RT-PCR was treated with 8 U of DNase I (RNase-free, NEB, M0303) for 15 min at 37 °C in a 50 μl reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was extracted and treated with DNase (DNase I, RNase-free, New England Biolabs, Ipswich, MA) according to the TRIzol reagent user guide (Pub ...
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bioRxiv - Synthetic Biology 2023Quote: ... suspended with nuclease-free water and directly used with HiScribe T7 RNA Polymerase kit (purchased from NEB). Full-length of transcribed product was purified via TBE:Urea PAGE gel ...
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bioRxiv - Microbiology 2023Quote: ... in vitro-transcribed Tat/Rev RNA samples were treated with RNase-free DNase I (New England Biolabs) and purified using the Monarch® RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Physiology 2024Quote: ... The flow cell was washed with nuclease-free water three times and incubated with DraI (R0129, NEB) and CIAP (M0525 ...
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bioRxiv - Microbiology 2023Quote: ... pellets dried and resuspended in 100 µL RNase-free water containing 40 U murine RNase inhibitor (NEB). Following quantification by Nanodrop ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting mixtures were loaded on a 6% native polyacrylamide gel with SDS-free loading dye (NEB). The gel was made with TAE-Mg buffer (0.4 M Tris ...
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bioRxiv - Synthetic Biology 2024Quote: GFP fluorescence during cell free expression was performed using 25 µl reactions with PurExpress (New England BioLabs) in 384-well black microplates ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eluted purified PCR products (45μl in nuclease-free water) were digested with a lambda exonuclease (NEB M0262L) and accompanying reaction buffer for 20 minutes at 37°C (total reaction volume 50μl) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μg/mL nuclease-free BSA and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs)) in a 37 °C water bath ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg plasmid was digested for 4 h at 37°C with BstXI and XhoI (New England Biolabs). Products were run on a 1% agarose gel for 30 min at 120 V ...
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bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated from 1 µg total RNA using the NEB Poly(A) mRNA Magnetic Isolation Module (NEB) and prepared using the Stranded RNA-seq protocol (67) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized on 2 µg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg total RNA was subjected to poly(A) mRNA isolation according to the manufacturer’s protocols (NEB, #E7490). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of DNA was incubated 20min at 37°C with 15 units of RNAseH1 (New England Biolabs) and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs) ...
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bioRxiv - Systems Biology 2023Quote: ... with 1.5 µg undigested genomic DNA per PCR reaction using Q5 HotStart High Fidelity Polymerase (New England Biolabs). Resulting PCR product from multiple reactions per sample were pooled ...
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bioRxiv - Cancer Biology 2024Quote: ... Dot blot samples were prepared from 1 µg gDNA with or without RNaseH1 2500 U treatment (NEB, M0297) O/N 37°C.Samples were denatured in Denaturation Buffer (NaOH 400 mM ...
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bioRxiv - Immunology 2022Quote: ... The nuclease-digested RNA was then dephosphorylated by adding 10 units/µg of Quick CIP enzyme (NEB, M0525) and incubating at 37 °C for 90 min (Kellner et al. ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with MluCI (5µl Cut smart buffer, 1.5-2 µg DNA, 3 µl MluCI (New England Biolabs Inc. (NEB), and water to make up 49 µl ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized on 2 µg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates (500 µg) were subjected to precleaning using 25 µl Protein A (New England Biolabs, Cat # S1425S) and G (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... and lysates were used as gDNA templates for PCR amplification with OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (all materials from New England BioLabs, Ipswich, MA). Genotyping was performed with primers listed in Supplemental Table 6 with the following thermocycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Bioengineering 2024Quote: ... OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (New England Biolabs catalog no. M0488S; New England BioLabs, Ipswich, MA), was used in a 25 µl reaction with 2 µl template DNA for amplification according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2022Quote: ... The labeled primer was purified by G-25 microspin column (Cytvia) and Monarch DNA purification kit (NEB). The successful conjugation of three digoxigenin moieties was confirmed by the shift of the molecular weight using denaturing urea polyacrylamide gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... The 3’ ends of the VSV G and GP64 ORFs were PCR-amplified (using Taq polymerase, NEB) from DNA isolated from each fly line ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Neuroscience 2022Quote: ... or 120 μM m7G(5’)ppp(5’)G RNA Cap Structure Analog (M7; New England BioLabs #S1404S) was used ...
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bioRxiv - Synthetic Biology 2024Quote: G(5’)ppp(5’)A RNA Cap Structure Analog (New England Biolabs Japan Inc., Tokyo, Japan, #S1406)
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bioRxiv - Microbiology 2024Quote: Ubiquitination reactions containing recombinant GST-PKN1 were immunoprecipitated using protein G-conjugated magnetic beads (New England Biolabs). Beads were prepared by washing three times with IP wash buffer (PBS + 0.1% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... Enzymatics) and end repaired Silane purified cDNA eluted in in 32 μl of RNase free H2O (1x NEB buffer 2 ...
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bioRxiv - Developmental Biology 2020Quote: ... the total RNA samples were treated with RNase-free deoxyribonuclease I (DNase I) (New England BioLabs, Beverly, MA) to remove residual DNA ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Systems Biology 2020Quote: Single hematopoietic stem and progenitor cells were index-sorted into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Up to 1 μg of DNA-free RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368) and anchored oligo dT primers according to the product instructions.
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bioRxiv - Immunology 2021Quote: ... 3.6 μL of exonuclease reaction solution (2.52 μL sterile nuclease-free water, 0.36 μL Exo reaction buffer, and 0.72 μL Exo-I, New England BioLabs) was then added to remove unincorporated primers (37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Five-hundred ng RNA in 9μl nuclease-free water was mixed with 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Immunology 2021Quote: ... into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted RNA was resuspended in 20 µL nuclease-free water and treated with DNase I (New England BioLabs) for 10 minutes at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega) ...
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bioRxiv - Biochemistry 2023Quote: Removal of N-linked oligosaccharides in mini-procollagens was performed with PNGase F (New England BioLabs, glycerol-free). Ten micrograms of mini-procollagens were first denatured at 100 °C for 10 min in Glycoprotein Denaturing Buffer provided with the enzyme ...