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Citations for New England Biolabs :
951 - 1000 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: 300 intestinal stem and Paneth cells were sorted into 2 μl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB). RNA was reverse transcribed (Invitrogen ...
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bioRxiv - Genomics 2021Quote: The genomic DNA library cloned into the pCpG-free-Sasaki-SS-v2 vector was methylated using bacterial CpG methylase M.SssI (NEB). The reaction was performed for 4 h at 37 C as per manufacturer’s recommendation with the reaction volumes scaled for 62.5 ug of plasmid DNA per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... with a single-cysteine at position 336 was created from the dimeric cysteine-free MutS variant (24,30,31) using the NEBuilder® HiFi Assembly Cloning Kit (NEB) (see Supplementary Data for details ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated by precipitation in isopropanol-ethanol 75% and resuspended in 10 μL nuclease free water (NEB, B1500) before quantification by BioDrop μLITE (Biodrop) ...
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bioRxiv - Microbiology 2022Quote: ... ShdA III and ShdA IV homologues were synthesised in vitro using the PURExpress cell-free transcription/translation kit (NEB), from PCR products ...
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bioRxiv - Biochemistry 2022Quote: ... by gradient salt dialysis in the presence of 0.2 mg/ml nuclease-free bovine serum albumin (BSA, New England Biolabs). Quality of reconstitution was assessed by MNase and chromatosome stop assays (Figure 2-figure supplement 1D&E).
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA for WGS was prepared by PCR-free library preparation according to the NEBNext DNA Ultra II kit (NEB). Sequencing was performed by Illumina NextSeq 2000 P3 150PE.
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bioRxiv - Microbiology 2024Quote: ... pellets dried and resuspended in 100 µl RNase-free water containing 40 U murine RNase inhibitor (New England Biolabs). Following quantification by Nanodrop ...
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bioRxiv - Microbiology 2024Quote: The extracted RNAs from the BCoV-13 were treated with DNase I (RNase-free) (New England BioLabs; Lot. # 10213692) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA was resuspended in an appropriate volume of RNase-free water supplemented with RNA inhibitors (New England Biolabs). RNA samples were stored at -80°C until further use ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were incubated at 37°C for 3 hours then digested with 64 units of RNase Free DNaseI (NEB) at 37°C for 15 minutes ...
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bioRxiv - Systems Biology 2024Quote: ... complete Protease Inhibitor EDTA-Free) with 5 minutes nutation and then resuspended in tagmentation buffer (1x rCutSmart Buffer NEB #B6004S ...
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bioRxiv - Biochemistry 2024Quote: ... the free DNA fragments were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) (New England Biolabs, #T1030S) and quantified ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg of total RNA using ProtoScriptR II Reverse Transcriptase (New England BioLabs, MA, USA) in 20 µl reaction with oligo (dT ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was isolated with Proteinase K lysis buffer and 15 µg of DNA were digested overnight with 40 units of MspI (New England Biolabs). Digested DNA was separated on a 0.8% agarose gel ...
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bioRxiv - Biochemistry 2020Quote: ... The region harboring the randomized hexamer and flanking constant tags was amplified from 1 µg ssDNA for 5 cycles using the Q5 Hot Start High-Fidelity PCR Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Genomics 2021Quote: The RNA-seq libraries were prepared as follows: the rRNA was depleted from 0.5 µg of A549 total RNA using the NEBNext rRNA Depletion Kit (NEB E6310). The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760) ...
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bioRxiv - Genomics 2021Quote: ... approximately 2.5 µg of high molecular weight DNA was repaired using the NEBNext FFPE Repair Mix (NEB, Ipswich, Ma, USA) for 15 min at 20°C before purification with Ampure XP beads ...
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bioRxiv - Cancer Biology 2020Quote: ... We performed eight 100 µl PCR reactions per sample (4 µg DNA per reaction) using Q5 High-Fidelity 2x Master Mix (New England Biolabs)28 to maximize library sequencing quality ...
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bioRxiv - Microbiology 2020Quote: ... 6 µg of DNA was used for MmeI digestion in 200 µL (6 µg gDNA, 6 µL MmeI (2000 U/mL, NEB), 0.5 µL 32 mM S-adenosylmethionine ...
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bioRxiv - Molecular Biology 2022Quote: ... 2019 [36] with the modification that 3 µg of gDNA per technical replicate were digested using Nucleoside Digestion Mix (NEB M0649S ...
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bioRxiv - Microbiology 2021Quote: ... We followed the input DNA mass recommendation of 1.0 µg gDNA and performed DNA repair (NEBNext FFPE DNA Repair Mix, NEB M6630) to maximize read length ...
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bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... to a final concentration of 0.1µg/ml was incubated with 3 µg purified STAT3 proteins as well as 5 µl ATP (New England Biolabs; N0440S) for 30 minutes at 30°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Transformations for integration onto p1 were performed as described previously:15 2–4 µg of plasmid DNA with ScaI restriction sites adjacent to integration flanks was cut with ScaI-HF (NEB) and transformed into yeast harboring the wt p1 and p2 plasmids ...
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bioRxiv - Plant Biology 2020Quote: ... and an aliquot containing 10 µg protein was treated with lambda protein phosphatase reaction mix following the instructions of the manufacturer (New England Biolabs) for 1 h at 30°C.
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bioRxiv - Genomics 2021Quote: ... The FPR cassette comprising NatMX-mCherry-PPT1/SUT129 promoter-YFP was excised from the backbone using AscI restriction enzyme (1 U per µg plasmid, NEB). Fragment I was the bidirectional GCG1/SUT098 or ORC2/SUT014 promoter sequences amplified by Phusion U polymerase from BY4741 genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... beads were resuspended in GRB buffer and incubated with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070 ...
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bioRxiv - Genomics 2021Quote: ... 1 µg total RNA was subjected to cDNA synthesis applying the ProtoScript® First Strand cDNA Synthesis Kit (NEB, E6300S) with the included Oligo d(T)23 VN primer according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with MluCI (5µl Cut smart buffer, 1.5-2 µg DNA, 3 µl MluCI (New England Biolabs Inc. (NEB), and water to make up 49 µl ...
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bioRxiv - Systems Biology 2024Quote: ... The samples then underwent two separate digestion reactions (with up to 4 µg of genomic DNA) using NlaIII and MseI enzymes (NEB) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... with 2 µg of the freshly synthetized SgRNA1 obtained with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). DNA repair templates containing the fenhexamid resistance cassette surrounded by 60 bp of the target gene for HR were obtained by PCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... DIG- and FITC-labeled antisense RNA probes were generated from 1 µg of linearized plasmid template using T7 or Sp6 RNA polymerases (NEB) and DIG-11-UTP (Roche) ...
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bioRxiv - Genomics 2024Quote: Each of the fragmented protein factor-enriched chromatin sample was mixed with 50 µg/ml of BSA (cat# B9000S, NEB) to prevent chromatin aggregation ...
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1% NP-40 buffer as above and 20 µg of lysate or 50 µL of supernatants were subjected to Endo H (P0702S NEB) or PNGase F (P0709S NEB ...
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bioRxiv - Plant Biology 2023Quote: ... And 1 µg of the total RNA was reverse transcribed into cDNA using Luna script RT Supermix (New England Biolabs) as per the manufacture’s protocol ...
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bioRxiv - Pathology 2023Quote: ... cDNA library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs). cDNA libraries were sequenced by NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Capped RNA was synthesized from the pCS2+hSpCas9 vector using an AmpliCap SP6 High Yield Message Maker Kit (Cell Script) and then purified with a Monarch RNA Cleanup Kit (50 µg) (New England BioLabs).
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bioRxiv - Biochemistry 2023Quote: ... Reactions with UDP-MurNAc-80mer RNA contained 2 µg RNA and products were purified using the Monarch RNA Cleanup Kit (NEB).
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CutSmart buffer (50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 µg/ml BSA or recombinant albumin; NEB), 0.5 mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Cancer Biology 2023Quote: Assessment of the integration status of the HPV-positive HNSCC cell lines was characterized for each cell line and digested with 7 µg of total genomic DNA at 37°C overnight (20 hours) with either EcoRV (New England BioLabs) or Bam HI ...
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bioRxiv - Pathology 2024Quote: ... cDNA library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs). cDNA libraries were sequenced by NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription was performed with 1 µg of RNA using one-step reaction using LunaScript RT SuperMix Kit (NEB, #E3010) as indicated by the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... a NASBA enzyme mix was prepared by combining the following components (listed at final concentration): 0.1 µg/µL BSA (NEB #B9000S), 5 U/µL T7 RNAP (NEB #M0460T) ...
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bioRxiv - Cancer Biology 2024Quote: ... perform 25-30 separate 100 µL reactions with 6-8 µg genomic DNA in each reaction using Q5 High-Fidelity DNA Polymerase (New England Biolabs) for around 18-20 cycles and then combine the resulting amplicons ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µg of total RNA was circularised in a reaction containing 6 U T4 RNA Ligase 1 (New England Biolabs), 50 µM ATP ...